Immune cell profiling of the cerebrospinal fluid enables the characterization of the brain metastasis microenvironment

Brain metastases are the most common tumor of the brain with a dismal prognosis. A fraction of patients with brain metastasis benefit from treatment with immune checkpoint inhibitors (ICI) and the degree and phenotype of the immune cell infiltration has been used to predict response to ICI. However,...

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Detalhes bibliográficos
Autores: Rubio-Perez, Carlota|||0000-0002-1921-168X, Planas-Rigol, Ester|||0000-0001-5948-2241, Trincado Alonso, Juan Luis, 1987-|||0000-0001-5363-3774, Bonfill-Teixidor, Ester|||0000-0002-9260-448X, Arias, Alexandra, Marchese, Domenica|||0000-0002-3036-4580, Moutinho, Catia|||0000-0003-3885-0847, Serna, Garazi|||0000-0003-2666-0982, Pedrosa, Leire|||0000-0002-1852-4975, Iurlaro, Raffaella|||0000-0003-1019-6679, Martinez-Ricarte, Fran|||0000-0003-4567-0828, Escudero, Laura|||0000-0001-7385-673X, Cordero Asanza, Esteban|||0000-0003-0464-3014, Cicuendez, Marta|||0000-0002-2603-9072, Ruiz, Sara, Parra, Genís, Nuciforo, Paolo|||0000-0003-1380-0990, Gonzalez, Josep, Pineda, Estela|||0000-0003-2128-747X, Sahuquillo Barris, Juan|||0000-0003-0713-5875, Tabernero, Josep|||0000-0002-2495-8139, Heyn, Holger|||0000-0002-3276-1889, Seoane Suárez, Joan|||0000-0002-6541-5974
Tipo de documento: artigo
Data de publicação:2021
País:España
Recursos:Universitat Autònoma de Barcelona
Repositório:Dipòsit Digital de Documents de la UAB
Idioma:inglês
OAI Identifier:oai:ddd.uab.cat:255343
Acesso em linha:https://ddd.uab.cat/record/255343
https://dx.doi.org/urn:doi:10.1038/s41467-021-21789-x
Access Level:Acceso aberto
Palavra-chave:Tumour immunology
Cancer microenvironment
Descrição
Resumo:Brain metastases are the most common tumor of the brain with a dismal prognosis. A fraction of patients with brain metastasis benefit from treatment with immune checkpoint inhibitors (ICI) and the degree and phenotype of the immune cell infiltration has been used to predict response to ICI. However, the anatomical location of brain lesions limits access to tumor material to characterize the immune phenotype. Here, we characterize immune cells present in brain lesions and matched cerebrospinal fluid (CSF) using single-cell RNA sequencing combined with T cell receptor genotyping. Tumor immune infiltration and specifically CD8 + T cell infiltration can be discerned through the analysis of the CSF. Consistently, identical T cell receptor clonotypes are detected in brain lesions and CSF, confirming cell exchange between these compartments. The analysis of immune cells of the CSF can provide a non-invasive alternative to predict the response to ICI, as well as identify the T cell receptor clonotypes present in brain metastasis. The use of CSF for diagnosis of metastatic brain tumors could be of clinical and patient benefit. Here the authors undertake a single-cell RNA analysis of CSF and brain to determine whether the phenotype in the CSF is reflective of the phenotype in the tumor

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