Traction forces at the cytokinetic ring regulate cell division and polyploidy in the migrating zebrafish epicardium

Epithelial repair and regeneration are driven by collective cell migration and division. Both cellular functions involve tightly controlled mechanical events, but how physical forces regulate cell division in migrating epithelia is largely unknown. Here we show that cells dividing in the migrating z...

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Autores: Uroz, Marina, Garcia Puig, Anna, Elosegui Artola, Alberto, Abenza, Juan F., Marín Llauradó, Ariadna, Pujals Riatós, Silvia, Conte, Vito, Albertazzi, Lorenzo, Roca-Cusachs Soulere, Pere, Raya Chamorro, Ángel, Trepat Guixer, Xavier
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2019
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/139578
Acceso en línea:https://hdl.handle.net/2445/139578
Access Level:acceso abierto
Palabra clave:Divisió cel·lular
Càncer
Cell division
Cancer
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spelling Traction forces at the cytokinetic ring regulate cell division and polyploidy in the migrating zebrafish epicardiumUroz, MarinaGarcia Puig, AnnaElosegui Artola, AlbertoAbenza, Juan F.Marín Llauradó, AriadnaPujals Riatós, SilviaConte, VitoAlbertazzi, LorenzoRoca-Cusachs Soulere, PereRaya Chamorro, ÁngelTrepat Guixer, XavierDivisió cel·lularCàncerCell divisionCancerEpithelial repair and regeneration are driven by collective cell migration and division. Both cellular functions involve tightly controlled mechanical events, but how physical forces regulate cell division in migrating epithelia is largely unknown. Here we show that cells dividing in the migrating zebrafish epicardium exert large cell–extracellular matrix (ECM) forces during cytokinesis. These forces point towards the division axis and are exerted through focal adhesions that connect the cytokinetic ring to the underlying ECM. When subjected to high loading rates, these cytokinetic focal adhesions prevent closure of the contractile ring, leading to multi-nucleation through cytokinetic failure. By combining a clutch model with experiments on substrates of different rigidity, ECM composition and ligand density, we show that failed cytokinesis is triggered by adhesion reinforcement downstream of increased myosin density. The mechanical interaction between the cytokinetic ring and the ECM thus provides a mechanism for the regulation of cell division and polyploidy that may have implications in regeneration and cancer.Springer Nature2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/139578Articles publicats en revistes (Biomedicina)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: https://doi.org/10.1038/s41563-019-0381-9Nature Materials, 2019, vol. 18https://doi.org/10.1038/s41563-019-0381-9info:eu-repo/grantAgreement/EC/FP7/616480info:eu-repo/grantAgreement/EC/H2020/731957cc by-nc-nd (c) uroz et al., 2019http://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1395782026-05-27T06:46:51Z
dc.title.none.fl_str_mv Traction forces at the cytokinetic ring regulate cell division and polyploidy in the migrating zebrafish epicardium
title Traction forces at the cytokinetic ring regulate cell division and polyploidy in the migrating zebrafish epicardium
spellingShingle Traction forces at the cytokinetic ring regulate cell division and polyploidy in the migrating zebrafish epicardium
Uroz, Marina
Divisió cel·lular
Càncer
Cell division
Cancer
title_short Traction forces at the cytokinetic ring regulate cell division and polyploidy in the migrating zebrafish epicardium
title_full Traction forces at the cytokinetic ring regulate cell division and polyploidy in the migrating zebrafish epicardium
title_fullStr Traction forces at the cytokinetic ring regulate cell division and polyploidy in the migrating zebrafish epicardium
title_full_unstemmed Traction forces at the cytokinetic ring regulate cell division and polyploidy in the migrating zebrafish epicardium
title_sort Traction forces at the cytokinetic ring regulate cell division and polyploidy in the migrating zebrafish epicardium
dc.creator.none.fl_str_mv Uroz, Marina
Garcia Puig, Anna
Elosegui Artola, Alberto
Abenza, Juan F.
Marín Llauradó, Ariadna
Pujals Riatós, Silvia
Conte, Vito
Albertazzi, Lorenzo
Roca-Cusachs Soulere, Pere
Raya Chamorro, Ángel
Trepat Guixer, Xavier
author Uroz, Marina
author_facet Uroz, Marina
Garcia Puig, Anna
Elosegui Artola, Alberto
Abenza, Juan F.
Marín Llauradó, Ariadna
Pujals Riatós, Silvia
Conte, Vito
Albertazzi, Lorenzo
Roca-Cusachs Soulere, Pere
Raya Chamorro, Ángel
Trepat Guixer, Xavier
author_role author
author2 Garcia Puig, Anna
Elosegui Artola, Alberto
Abenza, Juan F.
Marín Llauradó, Ariadna
Pujals Riatós, Silvia
Conte, Vito
Albertazzi, Lorenzo
Roca-Cusachs Soulere, Pere
Raya Chamorro, Ángel
Trepat Guixer, Xavier
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Divisió cel·lular
Càncer
Cell division
Cancer
topic Divisió cel·lular
Càncer
Cell division
Cancer
description Epithelial repair and regeneration are driven by collective cell migration and division. Both cellular functions involve tightly controlled mechanical events, but how physical forces regulate cell division in migrating epithelia is largely unknown. Here we show that cells dividing in the migrating zebrafish epicardium exert large cell–extracellular matrix (ECM) forces during cytokinesis. These forces point towards the division axis and are exerted through focal adhesions that connect the cytokinetic ring to the underlying ECM. When subjected to high loading rates, these cytokinetic focal adhesions prevent closure of the contractile ring, leading to multi-nucleation through cytokinetic failure. By combining a clutch model with experiments on substrates of different rigidity, ECM composition and ligand density, we show that failed cytokinesis is triggered by adhesion reinforcement downstream of increased myosin density. The mechanical interaction between the cytokinetic ring and the ECM thus provides a mechanism for the regulation of cell division and polyploidy that may have implications in regeneration and cancer.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/139578
url https://hdl.handle.net/2445/139578
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1038/s41563-019-0381-9
Nature Materials, 2019, vol. 18
https://doi.org/10.1038/s41563-019-0381-9
info:eu-repo/grantAgreement/EC/FP7/616480
info:eu-repo/grantAgreement/EC/H2020/731957
dc.rights.none.fl_str_mv cc by-nc-nd (c) uroz et al., 2019
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by-nc-nd (c) uroz et al., 2019
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv Articles publicats en revistes (Biomedicina)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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