Bile acid-binding capacity of peptide extracts obtained from chicken blood hydrolysates using HPLC

Bile acids are involved in the modulation of various metabolic processes facilitating the biliary excretion of endogenous and exogenous cholesterol. The objective of this study was to determine the glycocholic acid binding capacity (BC) of chicken blood hydrolysates using an optimized RP-HPLC method...

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Detalles Bibliográficos
Autores: Carrera-Alvarado, Gisela, Toldrá Vilardell, Fidel, Mora, Leticia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/287081
Acceso en línea:http://hdl.handle.net/10261/287081
https://api.elsevier.com/content/abstract/scopus_id/85145257821
Access Level:acceso abierto
Palabra clave:Anticholesterolemic activity
Bile acid binding capacity
Chicken blood hydrolysates
Hypocholesterolemic peptides
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spelling Bile acid-binding capacity of peptide extracts obtained from chicken blood hydrolysates using HPLCCarrera-Alvarado, GiselaToldrá Vilardell, FidelMora, LeticiaAnticholesterolemic activityBile acid binding capacityChicken blood hydrolysatesHypocholesterolemic peptidesBile acids are involved in the modulation of various metabolic processes facilitating the biliary excretion of endogenous and exogenous cholesterol. The objective of this study was to determine the glycocholic acid binding capacity (BC) of chicken blood hydrolysates using an optimized RP-HPLC methodology. Samples were hydrolysed using a combination of five different enzymes. Alcalase and Protamex hydrolysates presented the highest BC, with mean values of 20.09% and 20.61%, respectively. Subsequently, both hydrolysates were ultrafiltered to obtain fractions >10 kDa, between 10 and 3 kDa, and <3 kDa, and the highest BC values were obtained for peptide fractions >10 kDa. Finally, the protein fragments (MW > 10 kDa) potentially responsible for BC were identified by LC-MS/MS. The results confirmed the relation of BC with the molecular weight of the peptides generated, suggesting that certain protein fragments generated from chicken blood could contribute to a positive impact on health by interfering with cholesterol metabolism.Grant PID2020-119684RB-I00 funded by MCIN/AEI/10.13039/501100011033 is acknowledged. Grant GRISOLIAP/2020/021 de la Consellería de Innovació, Universitats, Ciència i Societat Digital de la Generalitat Valenciana (GCA) is also acknowledged. The proteomic analysis was performed in the proteomics facility of SCSIE University of Valencia belonging to Proteored.Peer reviewedElsevierMinisterio de Ciencia e Innovación (España)Generalitat Valenciana0000-0003-1571-5781Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/287081https://api.elsevier.com/content/abstract/scopus_id/85145257821reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-119684RB-I00LWTCarrera-Alvarado, G.; Toldrá, F.; Mora, L.; Data of manuscript Bile acid-binding capacity of peptide extracts obtained from chicken blood hydrolysates using HPLC; DIGITAL.CSIC;http://hdl.handle.net/10261/371902https://doi.org/10.1016/j.lwt.2022.114381Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2870812026-05-22T06:33:51Z
dc.title.none.fl_str_mv Bile acid-binding capacity of peptide extracts obtained from chicken blood hydrolysates using HPLC
title Bile acid-binding capacity of peptide extracts obtained from chicken blood hydrolysates using HPLC
spellingShingle Bile acid-binding capacity of peptide extracts obtained from chicken blood hydrolysates using HPLC
Carrera-Alvarado, Gisela
Anticholesterolemic activity
Bile acid binding capacity
Chicken blood hydrolysates
Hypocholesterolemic peptides
title_short Bile acid-binding capacity of peptide extracts obtained from chicken blood hydrolysates using HPLC
title_full Bile acid-binding capacity of peptide extracts obtained from chicken blood hydrolysates using HPLC
title_fullStr Bile acid-binding capacity of peptide extracts obtained from chicken blood hydrolysates using HPLC
title_full_unstemmed Bile acid-binding capacity of peptide extracts obtained from chicken blood hydrolysates using HPLC
title_sort Bile acid-binding capacity of peptide extracts obtained from chicken blood hydrolysates using HPLC
dc.creator.none.fl_str_mv Carrera-Alvarado, Gisela
Toldrá Vilardell, Fidel
Mora, Leticia
author Carrera-Alvarado, Gisela
author_facet Carrera-Alvarado, Gisela
Toldrá Vilardell, Fidel
Mora, Leticia
author_role author
author2 Toldrá Vilardell, Fidel
Mora, Leticia
author2_role author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Generalitat Valenciana
0000-0003-1571-5781
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Anticholesterolemic activity
Bile acid binding capacity
Chicken blood hydrolysates
Hypocholesterolemic peptides
topic Anticholesterolemic activity
Bile acid binding capacity
Chicken blood hydrolysates
Hypocholesterolemic peptides
description Bile acids are involved in the modulation of various metabolic processes facilitating the biliary excretion of endogenous and exogenous cholesterol. The objective of this study was to determine the glycocholic acid binding capacity (BC) of chicken blood hydrolysates using an optimized RP-HPLC methodology. Samples were hydrolysed using a combination of five different enzymes. Alcalase and Protamex hydrolysates presented the highest BC, with mean values of 20.09% and 20.61%, respectively. Subsequently, both hydrolysates were ultrafiltered to obtain fractions >10 kDa, between 10 and 3 kDa, and <3 kDa, and the highest BC values were obtained for peptide fractions >10 kDa. Finally, the protein fragments (MW > 10 kDa) potentially responsible for BC were identified by LC-MS/MS. The results confirmed the relation of BC with the molecular weight of the peptides generated, suggesting that certain protein fragments generated from chicken blood could contribute to a positive impact on health by interfering with cholesterol metabolism.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/287081
https://api.elsevier.com/content/abstract/scopus_id/85145257821
url http://hdl.handle.net/10261/287081
https://api.elsevier.com/content/abstract/scopus_id/85145257821
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-119684RB-I00
LWT
Carrera-Alvarado, G.; Toldrá, F.; Mora, L.; Data of manuscript Bile acid-binding capacity of peptide extracts obtained from chicken blood hydrolysates using HPLC; DIGITAL.CSIC;http://hdl.handle.net/10261/371902
https://doi.org/10.1016/j.lwt.2022.114381
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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