Ratiometric Nanothermometer Based on a Radical Excimer for In Vivo Sensing

Ratiometric fluorescent nanothermometers with near-infrared emission play an important role in in vivo sensing since they can be used as intracellular thermal sensing probes with high spatial resolution and high sensitivity, to investigate cellular functions of interest in diagnosis and therapy, whe...

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Autores: Blasi, Davide, Gonzalez Pato, Nerea , Rodríguez Rodríguez, Xavier, Díez Zabala, Íñigo, Srinivasan, Sumithra Y., Camarero, Núria, Esquivias, Oriol, Roldán, Mònica, Guasch, Judit, Laromaine, Anna, Gorostiza, Pau, Veciana, Jaume, Ratera, Immaculada
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/334518
Acceso en línea:http://hdl.handle.net/10261/334518
https://api.elsevier.com/content/abstract/scopus_id/85152784757
Access Level:acceso abierto
Palabra clave:Caenorhabditis elegans
Excimer emission
In vivo sensing
Luminescence
organic radical nanoparticles
Ratiometric nanothermometers
Trityl radicals
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dc.title.none.fl_str_mv Ratiometric Nanothermometer Based on a Radical Excimer for In Vivo Sensing
title Ratiometric Nanothermometer Based on a Radical Excimer for In Vivo Sensing
spellingShingle Ratiometric Nanothermometer Based on a Radical Excimer for In Vivo Sensing
Blasi, Davide
Caenorhabditis elegans
Excimer emission
In vivo sensing
Luminescence
organic radical nanoparticles
Ratiometric nanothermometers
Trityl radicals
title_short Ratiometric Nanothermometer Based on a Radical Excimer for In Vivo Sensing
title_full Ratiometric Nanothermometer Based on a Radical Excimer for In Vivo Sensing
title_fullStr Ratiometric Nanothermometer Based on a Radical Excimer for In Vivo Sensing
title_full_unstemmed Ratiometric Nanothermometer Based on a Radical Excimer for In Vivo Sensing
title_sort Ratiometric Nanothermometer Based on a Radical Excimer for In Vivo Sensing
dc.creator.none.fl_str_mv Blasi, Davide
Gonzalez Pato, Nerea 
Rodríguez Rodríguez, Xavier
Díez Zabala, Íñigo
Srinivasan, Sumithra Y.
Camarero, Núria
Esquivias, Oriol
Roldán, Mònica
Guasch, Judit
Laromaine, Anna
Gorostiza, Pau
Veciana, Jaume
Ratera, Immaculada
author Blasi, Davide
author_facet Blasi, Davide
Gonzalez Pato, Nerea 
Rodríguez Rodríguez, Xavier
Díez Zabala, Íñigo
Srinivasan, Sumithra Y.
Camarero, Núria
Esquivias, Oriol
Roldán, Mònica
Guasch, Judit
Laromaine, Anna
Gorostiza, Pau
Veciana, Jaume
Ratera, Immaculada
author_role author
author2 Gonzalez Pato, Nerea 
Rodríguez Rodríguez, Xavier
Díez Zabala, Íñigo
Srinivasan, Sumithra Y.
Camarero, Núria
Esquivias, Oriol
Roldán, Mònica
Guasch, Judit
Laromaine, Anna
Gorostiza, Pau
Veciana, Jaume
Ratera, Immaculada
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Generalitat de Catalunya
Centro de Investigación Biomédica en Red Bioingeniería, Biomateriales y Nanomedicina (España)
European Commission
Fundación Lucha contra la Ceguera
Max Planck Society
Agencia Estatal de Investigación (España)
Blasi, Davide [0000-0002-6887-3364]
Srinivasan, Sumithra Y. [0000-0002-0473-9801]
Camarero, Núria [0000-0003-3045-5180]
Esquivias, Oriol [0000-0001-6814-2724]
Guasch, Judith [0000-0002-3571-4711]
Laromaine, Anna [0000-0002-4764-0780]
Gorostiza, Pau [0000-0002-7268-5577]
Veciana, Jaume [0000-0003-1023-9923]
Ratera, Immaculada [0000-0002-1464-9789]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Caenorhabditis elegans
Excimer emission
In vivo sensing
Luminescence
organic radical nanoparticles
Ratiometric nanothermometers
Trityl radicals
topic Caenorhabditis elegans
Excimer emission
In vivo sensing
Luminescence
organic radical nanoparticles
Ratiometric nanothermometers
Trityl radicals
description Ratiometric fluorescent nanothermometers with near-infrared emission play an important role in in vivo sensing since they can be used as intracellular thermal sensing probes with high spatial resolution and high sensitivity, to investigate cellular functions of interest in diagnosis and therapy, where current approaches are not effective. Herein, the temperature-dependent fluorescence of organic nanoparticles is designed, synthesized, and studied based on the dual emission, generated by monomer and excimer species, of the tris(2,4,6-trichlorophenyl)methyl radical (TTM) doping organic nanoparticles (TTMd-ONPs), made of optically neutral tris(2,4,6-trichlorophenyl)methane (TTM-αH), acting as a matrix. The excimer emission intensity of TTMd-ONPs decreases with increasing temperatures whereas the monomer emission is almost independent and can be used as an internal reference. TTMd-ONPs show a great temperature sensitivity (3.4% K-1 at 328 K) and a wide temperature response at ambient conditions with excellent reversibility and high colloidal stability. In addition, TTMd-ONPs are not cytotoxic and their ratiometric outputs are unaffected by changes in the environment. Individual TTMd-ONPs are able to sense temperature changes at the nano-microscale. In vivo thermometry experiments in Caenorhabditis elegans (C. elegans) worms show that TTMd-ONPs can locally monitor internal body temperature changes with spatio-temporal resolution and high sensitivity, offering multiple applications in the biological nanothermometry field.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
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dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/334518
https://api.elsevier.com/content/abstract/scopus_id/85152784757
url http://hdl.handle.net/10261/334518
https://api.elsevier.com/content/abstract/scopus_id/85152784757
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Small (Weinheim an der Bergstrasse, Germany)
http://doi.org/10.1002/smll.202207806
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dc.publisher.none.fl_str_mv Wiley-VCH
publisher.none.fl_str_mv Wiley-VCH
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
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spelling Ratiometric Nanothermometer Based on a Radical Excimer for In Vivo SensingBlasi, DavideGonzalez Pato, Nerea Rodríguez Rodríguez, XavierDíez Zabala, ÍñigoSrinivasan, Sumithra Y.Camarero, NúriaEsquivias, OriolRoldán, MònicaGuasch, JuditLaromaine, AnnaGorostiza, PauVeciana, JaumeRatera, ImmaculadaCaenorhabditis elegansExcimer emissionIn vivo sensingLuminescenceorganic radical nanoparticlesRatiometric nanothermometersTrityl radicalsRatiometric fluorescent nanothermometers with near-infrared emission play an important role in in vivo sensing since they can be used as intracellular thermal sensing probes with high spatial resolution and high sensitivity, to investigate cellular functions of interest in diagnosis and therapy, where current approaches are not effective. Herein, the temperature-dependent fluorescence of organic nanoparticles is designed, synthesized, and studied based on the dual emission, generated by monomer and excimer species, of the tris(2,4,6-trichlorophenyl)methyl radical (TTM) doping organic nanoparticles (TTMd-ONPs), made of optically neutral tris(2,4,6-trichlorophenyl)methane (TTM-αH), acting as a matrix. The excimer emission intensity of TTMd-ONPs decreases with increasing temperatures whereas the monomer emission is almost independent and can be used as an internal reference. TTMd-ONPs show a great temperature sensitivity (3.4% K-1 at 328 K) and a wide temperature response at ambient conditions with excellent reversibility and high colloidal stability. In addition, TTMd-ONPs are not cytotoxic and their ratiometric outputs are unaffected by changes in the environment. Individual TTMd-ONPs are able to sense temperature changes at the nano-microscale. In vivo thermometry experiments in Caenorhabditis elegans (C. elegans) worms show that TTMd-ONPs can locally monitor internal body temperature changes with spatio-temporal resolution and high sensitivity, offering multiple applications in the biological nanothermometry field.D.B. and N.G.-P. contributed equally to this work. The authors are grateful for the financial support received from the Spanish Government (PID2020-115296RA-I00, PID2019-105622RB-I00, PDI2021-122645OB-I00, RTI2018-096273-B-I00 and PID2019-111493RB-I00) funded by MICIN and the “Ramón y Cajal” program (RYC-2017-22614), the Generalitat de Catalunya (SGR-918, 2017SGR765, SGR Cat 2021-00438, 2017-SGR-1442, and 2021-SGR-1410, 2021-SGR-00446), and the Networking Research Center on Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN). DB gratefully acknoledges the REFIN (Return for Future Innovation) action for funding, an ininitiative co - funded by European Union through the POR Puglia 2014 - 2020 (ID grant: 2455F798). This research was also supported by the European Union's Horizon 2020 research and innovation program H2020-MSCA-COFUND-2016 (DOC-FAM, grant agreement Nr. 754397), Human Brain Project WaveScaES (SGA2-785907 and SGA3-945539), DEEPER (ICT36-2020-101016787), and the Marie Skłodowka-Curie grant agreement Nr. 101007804 (Micro4Nano). Support from Fundaluce and CaixaHealth (ID 100010434) are also acknowledged. The work was supported as well by the Max Planck Society through the Max Planck Partner Group “Dynamic Biomimetics for Cancer Immunotherapy” in collaboration with the Max Planck for Medical Research (Heidelberg, Germany). ICMAB-CSIC acknowledges support from the Severo Ochoa Programme for Centres of Excellence in R&D (FUNFUTURE, CEX2019-000917-S). IBEC acknowledges support from the CERCA programme of AGAUR/Generalitat de Catalunya and the Severo Ochoa Programme. This work had been developed inside the “Biochemistry, Molecular Biology and Biomedicine” and “Materials Science” Ph.D. programs of UAB. Nerea Gonzalez-Pato acknowledges the financial support from the FPU fellowship (FPU17/02551) from the Spanish Ministry.With funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000917-S).Peer reviewedWiley-VCHMinisterio de Ciencia, Innovación y Universidades (España)Generalitat de CatalunyaCentro de Investigación Biomédica en Red Bioingeniería, Biomateriales y Nanomedicina (España)European CommissionFundación Lucha contra la CegueraMax Planck SocietyAgencia Estatal de Investigación (España)Blasi, Davide [0000-0002-6887-3364]Srinivasan, Sumithra Y. [0000-0002-0473-9801]Camarero, Núria [0000-0003-3045-5180]Esquivias, Oriol [0000-0001-6814-2724]Guasch, Judith [0000-0002-3571-4711]Laromaine, Anna [0000-0002-4764-0780]Gorostiza, Pau [0000-0002-7268-5577]Veciana, Jaume [0000-0003-1023-9923]Ratera, Immaculada [0000-0002-1464-9789]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/334518https://api.elsevier.com/content/abstract/scopus_id/85152784757reponame:DIGITAL.CSIC. 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