The role of the LysR-type transcription factor PacR in regulating nitrogen metabolism in Anabaena sp. PCC7120

Chemicals and CAS Registry Numbers carbon 7440-44-0 chlorophyll 1406-65-1, 15611-43-5 nitrogen 7727-37-9 Ammonium Compounds Bacterial Proteins Carbon Chlorophyll Nitrates Nitrogen Transcription Factors

Detalles Bibliográficos
Autores: Werner, Elisa, Huokko, Tuomas, Santana-Sánchez, Anita, Picossi, Silvia, Nikkanen, Lauri, Herrero, Antonia, Allahverdiyeva, Yagut
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/393393
Acceso en línea:http://hdl.handle.net/10261/393393
https://api.elsevier.com/content/abstract/scopus_id/105004578494
Access Level:acceso abierto
Palabra clave:http://metadata.un.org/sdg/6
Ensure availability and sustainable management of water and sanitation for all
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spelling The role of the LysR-type transcription factor PacR in regulating nitrogen metabolism in Anabaena sp. PCC7120Werner, ElisaHuokko, TuomasSantana-Sánchez, AnitaPicossi, SilviaNikkanen, LauriHerrero, AntoniaAllahverdiyeva, Yaguthttp://metadata.un.org/sdg/6Ensure availability and sustainable management of water and sanitation for allChemicals and CAS Registry Numbers carbon 7440-44-0 chlorophyll 1406-65-1, 15611-43-5 nitrogen 7727-37-9 Ammonium Compounds Bacterial Proteins Carbon Chlorophyll Nitrates Nitrogen Transcription FactorsIn the filamentous cyanobacterium Anabaena sp. PCC 7120, heterocyst formation is triggered by changes in the C/N-ratio and relies on transcriptional reprogramming. The transcription factor PacR is considered a global regulator of carbon assimilation under photoautotrophic conditions, influencing the carbon concentrating mechanism and photosynthesis. It plays a role in balancing reducing power generation while protecting the photosynthetic apparatus from oxidative damage. However, PacR also binds to promoters of genes associated with heterocyst formation, although the underlying mechanisms remain unclear. To explore this, we studied the response of a PacR-deletion mutant to a nitrogen source shift from ammonium to nitrate. The absence of PacR led to heterocyst formation in nitrate-containing media, as well as reduced growth and chlorophyll content. We observed impaired nitrate uptake and disrupted ammonium assimilation via the GS/GOGAT-cycle. This phenotype may stem from PacR-mediated regulation of key genes of nitrogen and carbon metabolism as well as photosynthesis. An impact on photosynthesis is also apparent in the mutant, including a slight decrease in the size of the photo-reducible Fed-pool, suggesting that a shortage of reducing equivalents may contribute to nitrogen metabolism impairment.We thank Tor Laurén at Åbo Akademi University for elemental analysis, and Anni Nieminen, John Finell and Toveann Ahlnäs from the Institute for Molecular Medicine Finland (FIMM) for providing metabolic measurements. We thank Janne Isojärvi and Bradley Koch for their assistance with analyzing and graphing RNA sequencing dataThis work was supported by the NovoNordisk Foundation (PhotoCat, project # NNF20OC0064371 to YA), by the Jane and Aatos Erkko Foundation (PhotoFactory project, to YA). EW and TH were partially supported by the University of Turku Graduate School (UTUGS) and by the Turku Collegium for Science, Medicine and Technology, respectively.Peer reviewedWiley-LissÅbo Akademi UniversityFinnish Institute for Molecular MedicineNovo Nordisk FoundationJane and Aatos Erkko FoundationUniversity of TurkuTurku Collegium for Science, Medicine and TechnologyWerner, Elisa [0009-0002-1674-0161]Huokko, Tuomas [0000-0003-4409-3651]Santana-Sánchez, Anita [0000-0002-1556-0321]Picossi, Silvia [0000-0003-1175-6228]Nikkanen, Lauri [0000-0002-7192-9322]Herrero, Antonia [0000-0003-1071-6590]Allahverdiyeva, Yagut [0000-0002-9262-1757]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionaplication/pdfhttp://hdl.handle.net/10261/393393https://api.elsevier.com/content/abstract/scopus_id/105004578494reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1111/ppl.70248Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3933932026-05-22T06:33:51Z
dc.title.none.fl_str_mv The role of the LysR-type transcription factor PacR in regulating nitrogen metabolism in Anabaena sp. PCC7120
title The role of the LysR-type transcription factor PacR in regulating nitrogen metabolism in Anabaena sp. PCC7120
spellingShingle The role of the LysR-type transcription factor PacR in regulating nitrogen metabolism in Anabaena sp. PCC7120
Werner, Elisa
http://metadata.un.org/sdg/6
Ensure availability and sustainable management of water and sanitation for all
title_short The role of the LysR-type transcription factor PacR in regulating nitrogen metabolism in Anabaena sp. PCC7120
title_full The role of the LysR-type transcription factor PacR in regulating nitrogen metabolism in Anabaena sp. PCC7120
title_fullStr The role of the LysR-type transcription factor PacR in regulating nitrogen metabolism in Anabaena sp. PCC7120
title_full_unstemmed The role of the LysR-type transcription factor PacR in regulating nitrogen metabolism in Anabaena sp. PCC7120
title_sort The role of the LysR-type transcription factor PacR in regulating nitrogen metabolism in Anabaena sp. PCC7120
dc.creator.none.fl_str_mv Werner, Elisa
Huokko, Tuomas
Santana-Sánchez, Anita
Picossi, Silvia
Nikkanen, Lauri
Herrero, Antonia
Allahverdiyeva, Yagut
author Werner, Elisa
author_facet Werner, Elisa
Huokko, Tuomas
Santana-Sánchez, Anita
Picossi, Silvia
Nikkanen, Lauri
Herrero, Antonia
Allahverdiyeva, Yagut
author_role author
author2 Huokko, Tuomas
Santana-Sánchez, Anita
Picossi, Silvia
Nikkanen, Lauri
Herrero, Antonia
Allahverdiyeva, Yagut
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Åbo Akademi University
Finnish Institute for Molecular Medicine
Novo Nordisk Foundation
Jane and Aatos Erkko Foundation
University of Turku
Turku Collegium for Science, Medicine and Technology
Werner, Elisa [0009-0002-1674-0161]
Huokko, Tuomas [0000-0003-4409-3651]
Santana-Sánchez, Anita [0000-0002-1556-0321]
Picossi, Silvia [0000-0003-1175-6228]
Nikkanen, Lauri [0000-0002-7192-9322]
Herrero, Antonia [0000-0003-1071-6590]
Allahverdiyeva, Yagut [0000-0002-9262-1757]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv http://metadata.un.org/sdg/6
Ensure availability and sustainable management of water and sanitation for all
topic http://metadata.un.org/sdg/6
Ensure availability and sustainable management of water and sanitation for all
description Chemicals and CAS Registry Numbers carbon 7440-44-0 chlorophyll 1406-65-1, 15611-43-5 nitrogen 7727-37-9 Ammonium Compounds Bacterial Proteins Carbon Chlorophyll Nitrates Nitrogen Transcription Factors
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/393393
https://api.elsevier.com/content/abstract/scopus_id/105004578494
url http://hdl.handle.net/10261/393393
https://api.elsevier.com/content/abstract/scopus_id/105004578494
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1111/ppl.70248
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv aplication/pdf
dc.publisher.none.fl_str_mv Wiley-Liss
publisher.none.fl_str_mv Wiley-Liss
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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