Thioredoxins m are major players in the multifaceted light-adaptive response in Arabidopsis thaliana
Thioredoxins (TRXs) are well-known redox signalling players, which carry out post-translational modifica-tions in target proteins. Chloroplast TRXs are divided into different types and have central roles in lightenergy uptake and the regulation of primary metabolism. The isoforms TRX m1, m2, and m4 f...
| Authors: | , , , , , |
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| Format: | article |
| Status: | Published version |
| Publication Date: | 2021 |
| Country: | España |
| Institution: | Universidad de Sevilla (US) |
| Repository: | idUS. Depósito de Investigación de la Universidad de Sevilla |
| OAI Identifier: | oai:idus.us.es:11441/183229 |
| Online Access: | https://hdl.handle.net/11441/183229 https://doi.org/10.1111/tpj.15429 |
| Access Level: | Open access |
| Keyword: | thioredoxins Arabidopsis thaliana chloroplast light acclimation redox regulation chlorophyllfluorescence anthocyanins. |
| Summary: | Thioredoxins (TRXs) are well-known redox signalling players, which carry out post-translational modifica-tions in target proteins. Chloroplast TRXs are divided into different types and have central roles in lightenergy uptake and the regulation of primary metabolism. The isoforms TRX m1, m2, and m4 from Arabidop-sis thaliana are considered functionally related. Knowing their key position in the hub of plant metabolism,we hypothesized that the impairment of the TRX m signalling would not only have harmful consequenceson chloroplast metabolism but also at different levels of plant development. To uncover the physiologicaland developmental processes that depend on TRX m signalling, we carried out a comprehensive study ofArabidopsis single, double, and triple mutants defective in the TRX m1, m2, and m4 proteins. As light andredox signalling are closely linked, we investigated the response to high light (HL) of the plants that aregradually compromised in TRX m signalling. We provide experimental evidence relating the lack of TRX mand the appearance of novel phenotypic features concerning mesophyll structure, stomata biogenesis, andstomatal conductance. We also report new data indicating that the isoforms of TRX m fine-tune theresponse to HL, including the accumulation of the protective pigment anthocyanin. These results revealnovel signalling functions for the TRX m and underline their importance for plant growth and fulfilment ofthe acclimation/response to HL conditions. |
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