Snake Venomics of the Arboreal Talamancan Palm-Pitviper, Bothriechis nubestris, Provides Clues on the Origin of a Phenotypic Dichotomy between Type‑I and Type-II Venoms. Supplementary Tables S1–S3

We report the biochemical and proteomic characterization of the venom of the arboreal Talamancan palm-pitviper, Bothriechis nubestris, a species endemic to north and central parts of the Costa Rican Cordillera de Talamanca, at 2100 to 3000 m above sea level. The Talamancan palm-pitviper venom arsena...

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Autores: Chacón, Johelen, Chaves-Araya, Stephanie, Mena, Gianni, Chang-Castillo, Arturo, Díaz, Cecilia, Bonilla, Fabián, Sasa, Mahmood, Calvete, Juan J., Sanz, Libia, Gutiérrez, José MaríaGutiérrez, José María, Lomonte, Bruno, Fernández, Julián
Tipo de recurso: conjunto de datos
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/392610
Acceso en línea:http://hdl.handle.net/10261/392610
https://doi.org/10.1021/acs.jproteome.4c01041.s003
Access Level:acceso abierto
Palabra clave:Bothriechis nubestris
ICP antivenom
Snake venomics
Talamancan palm-pitviper
MiRNA
Type-II dichotomy
Venom type-I
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spelling Snake Venomics of the Arboreal Talamancan Palm-Pitviper, Bothriechis nubestris, Provides Clues on the Origin of a Phenotypic Dichotomy between Type‑I and Type-II Venoms. Supplementary Tables S1–S3Supplementary Table S1: Peptides and proteins matched in B. nubestris venom in the B. nubestris and B. nigroviridis databasesSupplementary Table S2: Peptides gathered by bottom-up peptidomics and venomics of reduced and carbamidomethylated B. nubestris SDS-PAGE bands of venom fractions, and their match in the transcriptomic databases of B. nubestris and B. nigroviridisSupplementary Table S3: Relative quantification of B. nubestris venom peptidome and proteome (XLSX)Chacón, JohelenChaves-Araya, StephanieMena, GianniChang-Castillo, ArturoDíaz, CeciliaBonilla, FabiánSasa, MahmoodCalvete, Juan J.Sanz, LibiaGutiérrez, José MaríaGutiérrez, José MaríaLomonte, BrunoFernández, JuliánBothriechis nubestrisICP antivenomSnake venomicsTalamancan palm-pitviperMiRNAType-II dichotomyVenom type-IWe report the biochemical and proteomic characterization of the venom of the arboreal Talamancan palm-pitviper, Bothriechis nubestris, a species endemic to north and central parts of the Costa Rican Cordillera de Talamanca, at 2100 to 3000 m above sea level. The Talamancan palm-pitviper venom arsenal comprised the translated products of 26 unique transcripts into 10 toxin classes, where metalloproteinases PIIa and PIII represent the dominant components. In vitro, the venom proteolyzed azocasein and gelatin but showed no phospholipase A2 or human plasma coagulant activities. In vivo, B. nubestris venom exhibited an intravenous median lethal dose (LD50) of 21.5 (95% confidence interval: 15.6–29.5) μg/mouse, and a minimum hemorrhagic dose (MHD) of 1.85 μg. PoliVal-ICP antivenom neutralized the venom’s lethal activity with a potency of 9.7 mg of venom/g of antivenom and significantly reduced the hemorrhagic effect. Comparison of venom gland transcriptomes and venom proteomes of B. nubestris and its closest congeneric relative, B. nigroviridis, revealed that highly conserved venom gland transcriptomes are differentially processed by each species to produce divergent (type-I vs type-II, respectively) venoms. This phenomenon contributes to the remarkable venom phenotypic variability found across the palm-pitviper phylogeny. A possible mechanism for the occurrence of type-I/type-II venom phenotypic dichotomy is discussed.pr4c01041_si_003.xlsxPeer reviewedAmerican Chemical SocietyCalvete, Juan J. [0000-0001-5026-3122]Bonilla, FabiánSasa, MahmoodCalvete, Juan J.Gutiérrez, José MaríaLomonte, BrunoFernández, JuliánConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/datasethttp://purl.org/coar/resource_type/c_ddb1http://hdl.handle.net/10261/392610https://doi.org/10.1021/acs.jproteome.4c01041.s003reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésChacón, Johelen; Chaves-Araya, Stephanie; Mena, Gianni; Chang-Castillo, Arturo; Díaz, Cecilia; Bonilla, Fabián; Sasa, Mahmood; Calvete, Juan J.; Sanz, Libia; Gutierrez, José María; Lomonte, Bruno; Fernández, Julián. (2025). Snake Venomics of the Arboreal Talamancan Palm-Pitviper, Bothriechis nubestris, Provides Clues on the Origin of a Phenotypic Dichotomy between Type‑I and Type-II Venoms. Journal of Proteome Research 24(6):2801-2815. https://doi.org/10.1021/acs.jproteome.4c01041. http://hdl.handle.net/10261/392118https://doi.org/10.1021/acs.jproteome.4c01041.s003Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3926102026-05-22T06:33:51Z
dc.title.none.fl_str_mv Snake Venomics of the Arboreal Talamancan Palm-Pitviper, Bothriechis nubestris, Provides Clues on the Origin of a Phenotypic Dichotomy between Type‑I and Type-II Venoms. Supplementary Tables S1–S3
Supplementary Table S1: Peptides and proteins matched in B. nubestris venom in the B. nubestris and B. nigroviridis databases
Supplementary Table S2: Peptides gathered by bottom-up peptidomics and venomics of reduced and carbamidomethylated B. nubestris SDS-PAGE bands of venom fractions, and their match in the transcriptomic databases of B. nubestris and B. nigroviridis
Supplementary Table S3: Relative quantification of B. nubestris venom peptidome and proteome (XLSX)
title Snake Venomics of the Arboreal Talamancan Palm-Pitviper, Bothriechis nubestris, Provides Clues on the Origin of a Phenotypic Dichotomy between Type‑I and Type-II Venoms. Supplementary Tables S1–S3
spellingShingle Snake Venomics of the Arboreal Talamancan Palm-Pitviper, Bothriechis nubestris, Provides Clues on the Origin of a Phenotypic Dichotomy between Type‑I and Type-II Venoms. Supplementary Tables S1–S3
Chacón, Johelen
Bothriechis nubestris
ICP antivenom
Snake venomics
Talamancan palm-pitviper
MiRNA
Type-II dichotomy
Venom type-I
title_short Snake Venomics of the Arboreal Talamancan Palm-Pitviper, Bothriechis nubestris, Provides Clues on the Origin of a Phenotypic Dichotomy between Type‑I and Type-II Venoms. Supplementary Tables S1–S3
title_full Snake Venomics of the Arboreal Talamancan Palm-Pitviper, Bothriechis nubestris, Provides Clues on the Origin of a Phenotypic Dichotomy between Type‑I and Type-II Venoms. Supplementary Tables S1–S3
title_fullStr Snake Venomics of the Arboreal Talamancan Palm-Pitviper, Bothriechis nubestris, Provides Clues on the Origin of a Phenotypic Dichotomy between Type‑I and Type-II Venoms. Supplementary Tables S1–S3
title_full_unstemmed Snake Venomics of the Arboreal Talamancan Palm-Pitviper, Bothriechis nubestris, Provides Clues on the Origin of a Phenotypic Dichotomy between Type‑I and Type-II Venoms. Supplementary Tables S1–S3
title_sort Snake Venomics of the Arboreal Talamancan Palm-Pitviper, Bothriechis nubestris, Provides Clues on the Origin of a Phenotypic Dichotomy between Type‑I and Type-II Venoms. Supplementary Tables S1–S3
dc.creator.none.fl_str_mv Chacón, Johelen
Chaves-Araya, Stephanie
Mena, Gianni
Chang-Castillo, Arturo
Díaz, Cecilia
Bonilla, Fabián
Sasa, Mahmood
Calvete, Juan J.
Sanz, Libia
Gutiérrez, José MaríaGutiérrez, José María
Lomonte, Bruno
Fernández, Julián
author Chacón, Johelen
author_facet Chacón, Johelen
Chaves-Araya, Stephanie
Mena, Gianni
Chang-Castillo, Arturo
Díaz, Cecilia
Bonilla, Fabián
Sasa, Mahmood
Calvete, Juan J.
Sanz, Libia
Gutiérrez, José MaríaGutiérrez, José María
Lomonte, Bruno
Fernández, Julián
author_role author
author2 Chaves-Araya, Stephanie
Mena, Gianni
Chang-Castillo, Arturo
Díaz, Cecilia
Bonilla, Fabián
Sasa, Mahmood
Calvete, Juan J.
Sanz, Libia
Gutiérrez, José MaríaGutiérrez, José María
Lomonte, Bruno
Fernández, Julián
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Calvete, Juan J. [0000-0001-5026-3122]
Bonilla, Fabián
Sasa, Mahmood
Calvete, Juan J.
Gutiérrez, José María
Lomonte, Bruno
Fernández, Julián
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Bothriechis nubestris
ICP antivenom
Snake venomics
Talamancan palm-pitviper
MiRNA
Type-II dichotomy
Venom type-I
topic Bothriechis nubestris
ICP antivenom
Snake venomics
Talamancan palm-pitviper
MiRNA
Type-II dichotomy
Venom type-I
description We report the biochemical and proteomic characterization of the venom of the arboreal Talamancan palm-pitviper, Bothriechis nubestris, a species endemic to north and central parts of the Costa Rican Cordillera de Talamanca, at 2100 to 3000 m above sea level. The Talamancan palm-pitviper venom arsenal comprised the translated products of 26 unique transcripts into 10 toxin classes, where metalloproteinases PIIa and PIII represent the dominant components. In vitro, the venom proteolyzed azocasein and gelatin but showed no phospholipase A2 or human plasma coagulant activities. In vivo, B. nubestris venom exhibited an intravenous median lethal dose (LD50) of 21.5 (95% confidence interval: 15.6–29.5) μg/mouse, and a minimum hemorrhagic dose (MHD) of 1.85 μg. PoliVal-ICP antivenom neutralized the venom’s lethal activity with a potency of 9.7 mg of venom/g of antivenom and significantly reduced the hemorrhagic effect. Comparison of venom gland transcriptomes and venom proteomes of B. nubestris and its closest congeneric relative, B. nigroviridis, revealed that highly conserved venom gland transcriptomes are differentially processed by each species to produce divergent (type-I vs type-II, respectively) venoms. This phenomenon contributes to the remarkable venom phenotypic variability found across the palm-pitviper phylogeny. A possible mechanism for the occurrence of type-I/type-II venom phenotypic dichotomy is discussed.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/dataset
http://purl.org/coar/resource_type/c_ddb1
format dataset
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/392610
https://doi.org/10.1021/acs.jproteome.4c01041.s003
url http://hdl.handle.net/10261/392610
https://doi.org/10.1021/acs.jproteome.4c01041.s003
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Chacón, Johelen; Chaves-Araya, Stephanie; Mena, Gianni; Chang-Castillo, Arturo; Díaz, Cecilia; Bonilla, Fabián; Sasa, Mahmood; Calvete, Juan J.; Sanz, Libia; Gutierrez, José María; Lomonte, Bruno; Fernández, Julián. (2025). Snake Venomics of the Arboreal Talamancan Palm-Pitviper, Bothriechis nubestris, Provides Clues on the Origin of a Phenotypic Dichotomy between Type‑I and Type-II Venoms. Journal of Proteome Research 24(6):2801-2815. https://doi.org/10.1021/acs.jproteome.4c01041. http://hdl.handle.net/10261/392118
https://doi.org/10.1021/acs.jproteome.4c01041.s003
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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