Placental exosomes profile in maternal and fetal circulation in intrauterine growth restriction - Liquid biopsies to monitoring fetal growth

INTRODUCTION: Placenta-derived exosomes may represent an additional pathway by which the placenta communicates with the maternal system to induce maternal vascular adaptations to pregnancy and it may be affected during Fetal growth restriction (FGR). The objective of this study was to quantify the c...

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Autores: Miranda, Jezid, Paules, Cristina, Nair, Soumyalekshmi, Lai, Andrew, Palma, Carlos, Scholz-Romero, Katherin, Rice, Gregory E., Gratacós Solsona, Eduard, Crispi Brillas, Fàtima, Salomon, Carlos
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/123438
Acceso en línea:https://hdl.handle.net/2445/123438
Access Level:acceso abierto
Palabra clave:Embaràs
Placenta
Retard del creixement intrauterí
Pregnancy
Fetal growth retardation
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spelling Placental exosomes profile in maternal and fetal circulation in intrauterine growth restriction - Liquid biopsies to monitoring fetal growthMiranda, JezidPaules, CristinaNair, SoumyalekshmiLai, AndrewPalma, CarlosScholz-Romero, KatherinRice, Gregory E.Gratacós Solsona, EduardCrispi Brillas, FàtimaSalomon, CarlosEmbaràsPlacentaRetard del creixement intrauteríPregnancyPlacentaFetal growth retardationINTRODUCTION: Placenta-derived exosomes may represent an additional pathway by which the placenta communicates with the maternal system to induce maternal vascular adaptations to pregnancy and it may be affected during Fetal growth restriction (FGR). The objective of this study was to quantify the concentration of total and placenta-derived exosomes in maternal and fetal circulation in small fetuses classified as FGR or small for gestational age (SGA). METHODS: Prospective cohort study in singleton term gestations including 10 normally grown fetuses and 20 small fetuses, sub-classified into SGA and FGR accordingly to birth weight (BW) percentile and fetoplacental Doppler. Exosomes were isolated from maternal and fetal plasma and characterized by morphology, enrichment of exosomal proteins, and size distribution by electron microscopy, western blot, and nanoparticle tracking analysis, respectively. Total and specific placenta-derived exosomes were determined using quantum dots coupled with CD63+ve and placental-type alkaline phosphatase (PLAP)+ve antibodies, respectively. RESULTS: Maternal concentrations of CD63+ve and PLAP+ve exosomes were similar between the groups (all p > 0.05). However, there was a significant positive correlation between the ratio of placental-derived to total exosomes (PLAP+ve ratio) and BW percentile, [rho = 0.77 (95% CI: 0.57 to 0.89); p = 0.0001]. The contribution of placental exosomes to the total exosome concentration in maternal and fetal circulation showed a significant decrease among cases, with lower PLAP+ve ratios in FGR compared to controls and SGA cases. DISCUSSION: Quantification of placental exosomes in maternal plasma reflects fetal growth and it may be a useful indicator of placental function.Elsevier2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/123438Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: https://doi.org/10.1016/j.placenta.2018.02.006Placenta, 2018, vol. 64, p. 34-43https://doi.org/10.1016/j.placenta.2018.02.006cc-by-nc-nd (c) International Federation of Placenta Associations , 2018http://creativecommons.org/licenses/by-nc-nd/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1234382026-05-27T06:46:51Z
dc.title.none.fl_str_mv Placental exosomes profile in maternal and fetal circulation in intrauterine growth restriction - Liquid biopsies to monitoring fetal growth
title Placental exosomes profile in maternal and fetal circulation in intrauterine growth restriction - Liquid biopsies to monitoring fetal growth
spellingShingle Placental exosomes profile in maternal and fetal circulation in intrauterine growth restriction - Liquid biopsies to monitoring fetal growth
Miranda, Jezid
Embaràs
Placenta
Retard del creixement intrauterí
Pregnancy
Placenta
Fetal growth retardation
title_short Placental exosomes profile in maternal and fetal circulation in intrauterine growth restriction - Liquid biopsies to monitoring fetal growth
title_full Placental exosomes profile in maternal and fetal circulation in intrauterine growth restriction - Liquid biopsies to monitoring fetal growth
title_fullStr Placental exosomes profile in maternal and fetal circulation in intrauterine growth restriction - Liquid biopsies to monitoring fetal growth
title_full_unstemmed Placental exosomes profile in maternal and fetal circulation in intrauterine growth restriction - Liquid biopsies to monitoring fetal growth
title_sort Placental exosomes profile in maternal and fetal circulation in intrauterine growth restriction - Liquid biopsies to monitoring fetal growth
dc.creator.none.fl_str_mv Miranda, Jezid
Paules, Cristina
Nair, Soumyalekshmi
Lai, Andrew
Palma, Carlos
Scholz-Romero, Katherin
Rice, Gregory E.
Gratacós Solsona, Eduard
Crispi Brillas, Fàtima
Salomon, Carlos
author Miranda, Jezid
author_facet Miranda, Jezid
Paules, Cristina
Nair, Soumyalekshmi
Lai, Andrew
Palma, Carlos
Scholz-Romero, Katherin
Rice, Gregory E.
Gratacós Solsona, Eduard
Crispi Brillas, Fàtima
Salomon, Carlos
author_role author
author2 Paules, Cristina
Nair, Soumyalekshmi
Lai, Andrew
Palma, Carlos
Scholz-Romero, Katherin
Rice, Gregory E.
Gratacós Solsona, Eduard
Crispi Brillas, Fàtima
Salomon, Carlos
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Embaràs
Placenta
Retard del creixement intrauterí
Pregnancy
Placenta
Fetal growth retardation
topic Embaràs
Placenta
Retard del creixement intrauterí
Pregnancy
Placenta
Fetal growth retardation
description INTRODUCTION: Placenta-derived exosomes may represent an additional pathway by which the placenta communicates with the maternal system to induce maternal vascular adaptations to pregnancy and it may be affected during Fetal growth restriction (FGR). The objective of this study was to quantify the concentration of total and placenta-derived exosomes in maternal and fetal circulation in small fetuses classified as FGR or small for gestational age (SGA). METHODS: Prospective cohort study in singleton term gestations including 10 normally grown fetuses and 20 small fetuses, sub-classified into SGA and FGR accordingly to birth weight (BW) percentile and fetoplacental Doppler. Exosomes were isolated from maternal and fetal plasma and characterized by morphology, enrichment of exosomal proteins, and size distribution by electron microscopy, western blot, and nanoparticle tracking analysis, respectively. Total and specific placenta-derived exosomes were determined using quantum dots coupled with CD63+ve and placental-type alkaline phosphatase (PLAP)+ve antibodies, respectively. RESULTS: Maternal concentrations of CD63+ve and PLAP+ve exosomes were similar between the groups (all p > 0.05). However, there was a significant positive correlation between the ratio of placental-derived to total exosomes (PLAP+ve ratio) and BW percentile, [rho = 0.77 (95% CI: 0.57 to 0.89); p = 0.0001]. The contribution of placental exosomes to the total exosome concentration in maternal and fetal circulation showed a significant decrease among cases, with lower PLAP+ve ratios in FGR compared to controls and SGA cases. DISCUSSION: Quantification of placental exosomes in maternal plasma reflects fetal growth and it may be a useful indicator of placental function.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/123438
url https://hdl.handle.net/2445/123438
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1016/j.placenta.2018.02.006
Placenta, 2018, vol. 64, p. 34-43
https://doi.org/10.1016/j.placenta.2018.02.006
dc.rights.none.fl_str_mv cc-by-nc-nd (c) International Federation of Placenta Associations , 2018
http://creativecommons.org/licenses/by-nc-nd/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) International Federation of Placenta Associations , 2018
http://creativecommons.org/licenses/by-nc-nd/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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