Microarray analysis of gene expression in rams experimentally-infected with the virulent strain of Brucella ovis

This is an open-access article distributed under the terms of the Creative Commons Attribution License.-- et al.

Detalles Bibliográficos
Autores: Azevedo de Paula Antunes, João, Galindo, Ruth C., Kocan, Katherine M., Fuente, José de la, Megid, Jane
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/142186
Acceso en línea:http://hdl.handle.net/10261/142186
Access Level:acceso abierto
Palabra clave:Brucellosis
Gene expression
Rams
Sheep
Microarray
Brucella ovis
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spelling Microarray analysis of gene expression in rams experimentally-infected with the virulent strain of Brucella ovisAzevedo de Paula Antunes, JoãoGalindo, Ruth C.Kocan, Katherine M.Fuente, José de laMegid, JaneBrucellosisGene expressionRamsSheepMicroarrayBrucella ovisThis is an open-access article distributed under the terms of the Creative Commons Attribution License.-- et al.Microarray analysis of gene expression profiles of total mRNA was studied in rams experimentally-infected with the virulent strain of Brucella ovis (B. ovis). The tissues studied included reproductive organs (epididymus, testicles, ampolae, vesicular glands, bulbourethral glands) and a pool of lymph nodes (inguinal and scrotal). The microarray analysis of each tissue was done at three time points: acute infection (60 days post infection [dpi]), chronic infection phase I (120 dpi), and chronic infection phase II (240 dpi). The gene expression profiles associated to B. ovis infection were determined using the Affymetrix Bovine Genome Array and expression levels of infected and uninfected rams (control group, 0 dpi) were compared. Of the 23,000 genes analyzed in the microarrays, 139 during acute phase of infection, 930 during the chronic phase I and 744 genes from the chronic phase II were identified as Differentially Expressed Genes (DEGs). Thirty known genes and fourteen unknown genes were expressed during the three phases of infection in all tissues. The biological functions of these genes included immune cell trafficking, immunological disease, infectious disease, inflammatory disease, inflammatory response and cellular movement, and significant differences were observed at the three phases of infection and in all infected tissues. For the first analysis, 8 genes in common during the three time points and to all infected tissues were chosen for the microarray validation. Acute phase of infection supported the relevance of the ataxia telangiectasia-mutated ATMIN (ATM interactor) gene. RCHY1 (ring finger and CHY zinc finger domain containing 1), ANKFY1 (ankyrin repeat and FYVE domain containing 1) and EFEMP1 (EGF-containing fibulin-like extracellular matrix protein 1) were also highlighted by the analysis during chronic phase I of infection. ZRAB2 (Zinc finger, RAN-binding domain containing 2) was validated at chronic phase II. This study represents the first analysis of microarray gene expression in different tissues of rams infected with of B. ovis at various times during infection and many of the genes identified require further study. These results expand the knowledge of the pathogenesis of this B. ovis strain infection in rams and suggest new genes and pathways for further investigation.This work was supported by Fundação de Amparo a Pesquisa do Estado de São Paulo-FAPESP (2008/03962-0), and by Conselho Nacional de Desenvolvimento Científico e Tecnológico-CNPQ (559023/2010-3). J.M.A.P. Antunes was granted with a full tuition FAPESP scholarship (2008/03837-0) in Brazil, and a full tuition of Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-CAPES scholarship (Programa Institucional de Doutorado Sanduíche no Exterior, Processo BEX 0987∕11-5) in Spain.Peer ReviewedOMICS Publishing GroupConselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil)Fundação de Amparo à Pesquisa do Estado de São PauloCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2017201720152017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/142186reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.4172/2155-952X.1000203Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1421862026-05-22T06:33:51Z
dc.title.none.fl_str_mv Microarray analysis of gene expression in rams experimentally-infected with the virulent strain of Brucella ovis
title Microarray analysis of gene expression in rams experimentally-infected with the virulent strain of Brucella ovis
spellingShingle Microarray analysis of gene expression in rams experimentally-infected with the virulent strain of Brucella ovis
Azevedo de Paula Antunes, João
Brucellosis
Gene expression
Rams
Sheep
Microarray
Brucella ovis
title_short Microarray analysis of gene expression in rams experimentally-infected with the virulent strain of Brucella ovis
title_full Microarray analysis of gene expression in rams experimentally-infected with the virulent strain of Brucella ovis
title_fullStr Microarray analysis of gene expression in rams experimentally-infected with the virulent strain of Brucella ovis
title_full_unstemmed Microarray analysis of gene expression in rams experimentally-infected with the virulent strain of Brucella ovis
title_sort Microarray analysis of gene expression in rams experimentally-infected with the virulent strain of Brucella ovis
dc.creator.none.fl_str_mv Azevedo de Paula Antunes, João
Galindo, Ruth C.
Kocan, Katherine M.
Fuente, José de la
Megid, Jane
author Azevedo de Paula Antunes, João
author_facet Azevedo de Paula Antunes, João
Galindo, Ruth C.
Kocan, Katherine M.
Fuente, José de la
Megid, Jane
author_role author
author2 Galindo, Ruth C.
Kocan, Katherine M.
Fuente, José de la
Megid, Jane
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil)
Fundação de Amparo à Pesquisa do Estado de São Paulo
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Brucellosis
Gene expression
Rams
Sheep
Microarray
Brucella ovis
topic Brucellosis
Gene expression
Rams
Sheep
Microarray
Brucella ovis
description This is an open-access article distributed under the terms of the Creative Commons Attribution License.-- et al.
publishDate 2015
dc.date.none.fl_str_mv 2015
2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/142186
url http://hdl.handle.net/10261/142186
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.4172/2155-952X.1000203
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv OMICS Publishing Group
publisher.none.fl_str_mv OMICS Publishing Group
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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