Influence of Storage Time on the DNA Integrity and Viability of Spermatozoa of the Spider Crab Maja brachydactyla

Natural populations of the spider crab Maja brachydactyla constitute a fishery resource of great economic importance in many countries. As in the rest of eubrachyurans, the females of this species have ventral-type seminal receptacles where they store sperm from copulations. Sperm can be stored in t...

Descripción completa

Detalles Bibliográficos
Autores: Rodríguez-Pena, Elba, Suárez, Diego, Estévez, Maria Graciela, Verísimo-Amor, Patricia, Barreira, Noelia, Fernández, Luis, González-Tizón, Ana, Martínez-Lage, Andrés
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/340185
Acceso en línea:http://hdl.handle.net/10261/340185
Access Level:acceso abierto
Palabra clave:Sperm storage time
Sperm viability
Maja brachydactyla
Comet assay
Sperm degradation
id ES_485e108a140cab6392cd26c8a8ebfa83
oai_identifier_str oai:digital.csic.es:10261/340185
network_acronym_str ES
network_name_str España
repository_id_str
spelling Influence of Storage Time on the DNA Integrity and Viability of Spermatozoa of the Spider Crab Maja brachydactylaRodríguez-Pena, ElbaSuárez, DiegoEstévez, Maria GracielaVerísimo-Amor, PatriciaBarreira, NoeliaFernández, LuisGonzález-Tizón, AnaMartínez-Lage, AndrésSperm storage timeSperm viabilityMaja brachydactylaComet assaySperm degradationNatural populations of the spider crab Maja brachydactyla constitute a fishery resource of great economic importance in many countries. As in the rest of eubrachyurans, the females of this species have ventral-type seminal receptacles where they store sperm from copulations. Sperm can be stored in these structures for months and even years before egg fertilisation, with the consequent degradation of the sperm cells during the time. In this work, we analyse the viability and the possible genetic damage in sperm accumulated in the seminal receptacles of M. brachydactyla females as a function of the storage time (from 0 to 14 months) using the comet assay technique. On one hand, we developed an algorithm for comet image analysis that improves the comet segmentation compared with the free software Open comet v1.3.1 (97% vs. 76% of detection). In addition, our software allows the manual modification of the contours wrongly delimited via the automatic tool. On the other hand, our data show a sharp decline in sperm viability and DNA integrity in the first four months of storage, which could lead to a decrease in the fecundity rate and/or viability of the embryos or larvae from the second and third clutches of the annual cycle if the repair capacity in these gametic cells is low.This research was funded by the Ministerio de Ciencia, Innovación y Universidades, grant number PID2019-109194RB-I00 MAR; and by the Ministerio de Ciencia e Innovación, grant number PID2020-113578RB-I00. It has also been supported by the Xunta de Galicia (Grupos de Referencia Competitiva ED431C-2020/14).Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Xunta de GaliciaEstévez, Maria Graciela [0000-0002-6021-5236]Barreira, Noelia [0000-0003-0961-2057]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2023202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/340185reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-109194RB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113578RB-I00https://doi.org/10.3390/ani13223555Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3401852026-05-22T06:33:51Z
dc.title.none.fl_str_mv Influence of Storage Time on the DNA Integrity and Viability of Spermatozoa of the Spider Crab Maja brachydactyla
title Influence of Storage Time on the DNA Integrity and Viability of Spermatozoa of the Spider Crab Maja brachydactyla
spellingShingle Influence of Storage Time on the DNA Integrity and Viability of Spermatozoa of the Spider Crab Maja brachydactyla
Rodríguez-Pena, Elba
Sperm storage time
Sperm viability
Maja brachydactyla
Comet assay
Sperm degradation
title_short Influence of Storage Time on the DNA Integrity and Viability of Spermatozoa of the Spider Crab Maja brachydactyla
title_full Influence of Storage Time on the DNA Integrity and Viability of Spermatozoa of the Spider Crab Maja brachydactyla
title_fullStr Influence of Storage Time on the DNA Integrity and Viability of Spermatozoa of the Spider Crab Maja brachydactyla
title_full_unstemmed Influence of Storage Time on the DNA Integrity and Viability of Spermatozoa of the Spider Crab Maja brachydactyla
title_sort Influence of Storage Time on the DNA Integrity and Viability of Spermatozoa of the Spider Crab Maja brachydactyla
dc.creator.none.fl_str_mv Rodríguez-Pena, Elba
Suárez, Diego
Estévez, Maria Graciela
Verísimo-Amor, Patricia
Barreira, Noelia
Fernández, Luis
González-Tizón, Ana
Martínez-Lage, Andrés
author Rodríguez-Pena, Elba
author_facet Rodríguez-Pena, Elba
Suárez, Diego
Estévez, Maria Graciela
Verísimo-Amor, Patricia
Barreira, Noelia
Fernández, Luis
González-Tizón, Ana
Martínez-Lage, Andrés
author_role author
author2 Suárez, Diego
Estévez, Maria Graciela
Verísimo-Amor, Patricia
Barreira, Noelia
Fernández, Luis
González-Tizón, Ana
Martínez-Lage, Andrés
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Xunta de Galicia
Estévez, Maria Graciela [0000-0002-6021-5236]
Barreira, Noelia [0000-0003-0961-2057]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Sperm storage time
Sperm viability
Maja brachydactyla
Comet assay
Sperm degradation
topic Sperm storage time
Sperm viability
Maja brachydactyla
Comet assay
Sperm degradation
description Natural populations of the spider crab Maja brachydactyla constitute a fishery resource of great economic importance in many countries. As in the rest of eubrachyurans, the females of this species have ventral-type seminal receptacles where they store sperm from copulations. Sperm can be stored in these structures for months and even years before egg fertilisation, with the consequent degradation of the sperm cells during the time. In this work, we analyse the viability and the possible genetic damage in sperm accumulated in the seminal receptacles of M. brachydactyla females as a function of the storage time (from 0 to 14 months) using the comet assay technique. On one hand, we developed an algorithm for comet image analysis that improves the comet segmentation compared with the free software Open comet v1.3.1 (97% vs. 76% of detection). In addition, our software allows the manual modification of the contours wrongly delimited via the automatic tool. On the other hand, our data show a sharp decline in sperm viability and DNA integrity in the first four months of storage, which could lead to a decrease in the fecundity rate and/or viability of the embryos or larvae from the second and third clutches of the annual cycle if the repair capacity in these gametic cells is low.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/340185
url http://hdl.handle.net/10261/340185
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-109194RB-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113578RB-I00
https://doi.org/10.3390/ani13223555
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869407355226030080
score 15,811543

Similares en LA Referencia