Biomimetic versus sintered calcium phosphates: The in vitro behavior of osteoblasts and mesenchymal stem cells

© Copyright 2017, Mary Ann Liebert, Inc.The fabrication of calcium phosphates using biomimetic routes, namely, precipitation processes at body temperature, results in distinct features compared to conventional sintered calcium phosphate ceramics, such as a high specific surface area (SSA) and micro-...

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Autores: Sadowska, Joanna Maria|||0000-0002-6252-0752, Guillem Martí, Jordi|||0000-0003-0307-2221, Montufar Jiménez, Edgar Benjamin, Español Pons, Montserrat|||0000-0001-7510-0602, Ginebra Molins, Maria Pau|||0000-0002-4700-5621
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/113924
Acceso en línea:https://hdl.handle.net/2117/113924
https://dx.doi.org/10.1089/ten.tea.2016.0406
Access Level:acceso abierto
Palabra clave:Biomedical materials
Biomimetic hydroxyapatite
Calcium phosphate
Mesenchymal stem cell
Osteoblast
Biomaterials
Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials
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spelling Biomimetic versus sintered calcium phosphates: The in vitro behavior of osteoblasts and mesenchymal stem cellsSadowska, Joanna Maria|||0000-0002-6252-0752Guillem Martí, Jordi|||0000-0003-0307-2221Montufar Jiménez, Edgar BenjaminEspañol Pons, Montserrat|||0000-0001-7510-0602Ginebra Molins, Maria Pau|||0000-0002-4700-5621Biomedical materialsBiomimetic hydroxyapatiteCalcium phosphateMesenchymal stem cellOsteoblastBiomaterialsÀrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials© Copyright 2017, Mary Ann Liebert, Inc.The fabrication of calcium phosphates using biomimetic routes, namely, precipitation processes at body temperature, results in distinct features compared to conventional sintered calcium phosphate ceramics, such as a high specific surface area (SSA) and micro-or nanometric crystal size. The aim of this article is to analyze the effects of these parameters on cell response, focusing on two bone cell types: rat mesenchymal stem cells (rMSCs) and human osteoblastic cells (SaOS-2). Biomimetic calcium-deficient hydroxyapatite (CDHA) was obtained by a low temperature setting reaction, and a-Tricalcium phosphate (a-TCP) and ß-Tricalcium phosphate were subsequently obtained by sintering CDHA either at 1400°C or 1100°C. Sintered stoichiometric hydroxyapatite (HA) was also prepared using ceramic routes. The materials were characterized in terms of SSA, skeletal density, porosity, and pore size distribution. SaOS-2 cells and rMSCs were seeded either directly on the surfaces of the materials or on glass coverslips subsequently placed on top of the materials to expose the cells to the CaP-induced ionic changes in the culture medium, while avoiding any topography-related effects. CDHA produced higher ionic fluctuations in both cell culture media than sintered ceramics, with a strong decrease of calcium and a release of phosphate. Indirect contact cell cultures revealed that both cell types were sensitive to these ionic modifications, resulting in a decrease in proliferation rate, more marked for CDHA, this effect being more pronounced for rMSCs. In direct contact cultures, good cell adhesion was found on all materials, but, while cells were able to proliferate on the sintered calcium phosphates, cell number was significantly reduced with time on biomimetic CDHA, which was associated to a higher percentage of apoptotic cells. Direct contact of the cells with biomimetic CDHA resulted also in a higher alkaline phosphatase activity for both cell types compared to sintered CaPs, indicating a promotion of the osteoblastic phenotype.Peer Reviewed20172017-12-0120182018-02-07journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/113924https://dx.doi.org/10.1089/ten.tea.2016.0406reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivs 3.0 Spainhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/1139242026-05-27T15:37:01Z
dc.title.none.fl_str_mv Biomimetic versus sintered calcium phosphates: The in vitro behavior of osteoblasts and mesenchymal stem cells
title Biomimetic versus sintered calcium phosphates: The in vitro behavior of osteoblasts and mesenchymal stem cells
spellingShingle Biomimetic versus sintered calcium phosphates: The in vitro behavior of osteoblasts and mesenchymal stem cells
Sadowska, Joanna Maria|||0000-0002-6252-0752
Biomedical materials
Biomimetic hydroxyapatite
Calcium phosphate
Mesenchymal stem cell
Osteoblast
Biomaterials
Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials
title_short Biomimetic versus sintered calcium phosphates: The in vitro behavior of osteoblasts and mesenchymal stem cells
title_full Biomimetic versus sintered calcium phosphates: The in vitro behavior of osteoblasts and mesenchymal stem cells
title_fullStr Biomimetic versus sintered calcium phosphates: The in vitro behavior of osteoblasts and mesenchymal stem cells
title_full_unstemmed Biomimetic versus sintered calcium phosphates: The in vitro behavior of osteoblasts and mesenchymal stem cells
title_sort Biomimetic versus sintered calcium phosphates: The in vitro behavior of osteoblasts and mesenchymal stem cells
dc.creator.none.fl_str_mv Sadowska, Joanna Maria|||0000-0002-6252-0752
Guillem Martí, Jordi|||0000-0003-0307-2221
Montufar Jiménez, Edgar Benjamin
Español Pons, Montserrat|||0000-0001-7510-0602
Ginebra Molins, Maria Pau|||0000-0002-4700-5621
author Sadowska, Joanna Maria|||0000-0002-6252-0752
author_facet Sadowska, Joanna Maria|||0000-0002-6252-0752
Guillem Martí, Jordi|||0000-0003-0307-2221
Montufar Jiménez, Edgar Benjamin
Español Pons, Montserrat|||0000-0001-7510-0602
Ginebra Molins, Maria Pau|||0000-0002-4700-5621
author_role author
author2 Guillem Martí, Jordi|||0000-0003-0307-2221
Montufar Jiménez, Edgar Benjamin
Español Pons, Montserrat|||0000-0001-7510-0602
Ginebra Molins, Maria Pau|||0000-0002-4700-5621
author2_role author
author
author
author
dc.subject.none.fl_str_mv Biomedical materials
Biomimetic hydroxyapatite
Calcium phosphate
Mesenchymal stem cell
Osteoblast
Biomaterials
Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials
topic Biomedical materials
Biomimetic hydroxyapatite
Calcium phosphate
Mesenchymal stem cell
Osteoblast
Biomaterials
Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials
description © Copyright 2017, Mary Ann Liebert, Inc.The fabrication of calcium phosphates using biomimetic routes, namely, precipitation processes at body temperature, results in distinct features compared to conventional sintered calcium phosphate ceramics, such as a high specific surface area (SSA) and micro-or nanometric crystal size. The aim of this article is to analyze the effects of these parameters on cell response, focusing on two bone cell types: rat mesenchymal stem cells (rMSCs) and human osteoblastic cells (SaOS-2). Biomimetic calcium-deficient hydroxyapatite (CDHA) was obtained by a low temperature setting reaction, and a-Tricalcium phosphate (a-TCP) and ß-Tricalcium phosphate were subsequently obtained by sintering CDHA either at 1400°C or 1100°C. Sintered stoichiometric hydroxyapatite (HA) was also prepared using ceramic routes. The materials were characterized in terms of SSA, skeletal density, porosity, and pore size distribution. SaOS-2 cells and rMSCs were seeded either directly on the surfaces of the materials or on glass coverslips subsequently placed on top of the materials to expose the cells to the CaP-induced ionic changes in the culture medium, while avoiding any topography-related effects. CDHA produced higher ionic fluctuations in both cell culture media than sintered ceramics, with a strong decrease of calcium and a release of phosphate. Indirect contact cell cultures revealed that both cell types were sensitive to these ionic modifications, resulting in a decrease in proliferation rate, more marked for CDHA, this effect being more pronounced for rMSCs. In direct contact cultures, good cell adhesion was found on all materials, but, while cells were able to proliferate on the sintered calcium phosphates, cell number was significantly reduced with time on biomimetic CDHA, which was associated to a higher percentage of apoptotic cells. Direct contact of the cells with biomimetic CDHA resulted also in a higher alkaline phosphatase activity for both cell types compared to sintered CaPs, indicating a promotion of the osteoblastic phenotype.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-12-01
2018
2018-02-07
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/113924
https://dx.doi.org/10.1089/ten.tea.2016.0406
url https://hdl.handle.net/2117/113924
https://dx.doi.org/10.1089/ten.tea.2016.0406
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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