Merging bioactuation and biocapacitive properties: a 3D bioprinted devices to self-stimulate using self-stored energy

Biofabrication of three-dimensional (3D) cultures through the 3D Bioprinting technique opens new perspectives and applications of cell-laden hydrogels. However, to continue with the progress, new BioInks with specific properties must be carefully designed. In this study, we report the synthesis and...

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Detalles Bibliográficos
Autores: Molina García, Brenda Guadalupe|||0000-0002-7723-5313, Fuentes Llanos, Judith, Alemán Llansó, Carlos|||0000-0003-4462-6075, Sanchez Ordoñez, Samuel
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/409051
Acceso en línea:https://hdl.handle.net/2117/409051
https://dx.doi.org/10.1016/j.bios.2024.116117
Access Level:acceso abierto
Palabra clave:Biosensors
C2C12 myoblasts
PEDOT NPs
3D bioprinting
Electroconductive BioInk
BioActuator
BioCapacitor
Àrees temàtiques de la UPC::Enginyeria química
Descripción
Sumario:Biofabrication of three-dimensional (3D) cultures through the 3D Bioprinting technique opens new perspectives and applications of cell-laden hydrogels. However, to continue with the progress, new BioInks with specific properties must be carefully designed. In this study, we report the synthesis and 3D Bioprinting of an electroconductive BioInk made of gelatin/fibrinogen hydrogel, C2C12 mouse myoblast and 5% w/w of conductive poly (3,4-ethylenedioxythiophene) nanoparticles (PEDOT NPs). The influence of PEDOT NPs, incorporated in the cell-laden BioInk, not only showed a positive effect in cells viability, differentiation and myotube functionalities, also allowed the printed constructs to behaved as BioCapacitors. Such devices were able to electrochemically store a significant amount of energy (0.5 mF/cm2), enough to self-stimulate as BioActuator, with typical contractions ranging from 27 to 38 µN, during nearly 50 min. The biofabrication of 3D constructs with the proposed electroconductive BioInk could lead to new devices for tissue engineering, biohybrid robotics or bioelectronics.