Temperature sensing using fluorescent nanothermometers

Acquiring the temperature of a single living cell is not a trivial task. In this paper, we devise a novel nanothermometer, capable of accurately determining the temperature of solutions as well as biological systems such as HeLa cancer cells. The nanothermometer is based on the temperature-sensitive...

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Detalles Bibliográficos
Autores: Vetrone, F., Naccache, R., Zamarrón, A., De La Fuente, A.J., Sanz-Rodríguez, F., Martínez Maestro, Laura, Rodriguez, E.M., Jaque, D., Sole, J.G., Capobianco, J.A.
Tipo de recurso: artículo
Fecha de publicación:2010
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/96493
Acceso en línea:https://hdl.handle.net/20.500.14352/96493
Access Level:acceso abierto
Palabra clave:620.1
Nanothermometer
Upconversion
HeLa cancer cell
Nanoparticles
Thermal sensing
Física de materiales
2211.16 Luminiscencia
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oai_identifier_str oai:docta.ucm.es:20.500.14352/96493
network_acronym_str ES
network_name_str España
repository_id_str
spelling Temperature sensing using fluorescent nanothermometersVetrone, F.Naccache, R.Zamarrón, A.De La Fuente, A.J.Sanz-Rodríguez, F.Martínez Maestro, LauraRodriguez, E.M.Jaque, D.Sole, J.G.Capobianco, J.A.620.1NanothermometerUpconversionHeLa cancer cellNanoparticlesThermal sensingFísica de materiales2211.16 LuminiscenciaAcquiring the temperature of a single living cell is not a trivial task. In this paper, we devise a novel nanothermometer, capable of accurately determining the temperature of solutions as well as biological systems such as HeLa cancer cells. The nanothermometer is based on the temperature-sensitive fluorescence of NaYF4:Er3+,Yb3+ nanoparticles, where the intensity ratio of the green fluorescence bands of the Er3+ dopant ions (H-2(11/2) -> I-4(15/2) and S-4(3/2) -> I-4(15/2)) changes with temperature. The nanothermometers were first used to obtain thermal profiles created when heating a colloidal solution of NaYF4:Er3+,Yb3+ nanoparticles in water using a pump-probe experiment. Following incubation of the nanoparticles with HeLa cervical cancer cells and their subsequent uptake, the fluorescent nanothermometers measured the internal temperature of the living cell from 25 degrees C to its thermally induced death at 45 degrees C.American Chemical SocietyUniversidad Complutense de Madrid20102010-01-0120102010-01-01journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/96493reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)InglésengMAT 2007-64686 Not available Not availableMinisterio de Educación PR2009-0049 ESCCG087-UAM MAT-4434 Not availableComunidad de Madrid http://dx.doi.org/10.13039/100012818 S2009 FMAT-1756open accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/964932026-06-02T12:44:21Z
dc.title.none.fl_str_mv Temperature sensing using fluorescent nanothermometers
title Temperature sensing using fluorescent nanothermometers
spellingShingle Temperature sensing using fluorescent nanothermometers
Vetrone, F.
620.1
Nanothermometer
Upconversion
HeLa cancer cell
Nanoparticles
Thermal sensing
Física de materiales
2211.16 Luminiscencia
title_short Temperature sensing using fluorescent nanothermometers
title_full Temperature sensing using fluorescent nanothermometers
title_fullStr Temperature sensing using fluorescent nanothermometers
title_full_unstemmed Temperature sensing using fluorescent nanothermometers
title_sort Temperature sensing using fluorescent nanothermometers
dc.creator.none.fl_str_mv Vetrone, F.
Naccache, R.
Zamarrón, A.
De La Fuente, A.J.
Sanz-Rodríguez, F.
Martínez Maestro, Laura
Rodriguez, E.M.
Jaque, D.
Sole, J.G.
Capobianco, J.A.
author Vetrone, F.
author_facet Vetrone, F.
Naccache, R.
Zamarrón, A.
De La Fuente, A.J.
Sanz-Rodríguez, F.
Martínez Maestro, Laura
Rodriguez, E.M.
Jaque, D.
Sole, J.G.
Capobianco, J.A.
author_role author
author2 Naccache, R.
Zamarrón, A.
De La Fuente, A.J.
Sanz-Rodríguez, F.
Martínez Maestro, Laura
Rodriguez, E.M.
Jaque, D.
Sole, J.G.
Capobianco, J.A.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 620.1
Nanothermometer
Upconversion
HeLa cancer cell
Nanoparticles
Thermal sensing
Física de materiales
2211.16 Luminiscencia
topic 620.1
Nanothermometer
Upconversion
HeLa cancer cell
Nanoparticles
Thermal sensing
Física de materiales
2211.16 Luminiscencia
description Acquiring the temperature of a single living cell is not a trivial task. In this paper, we devise a novel nanothermometer, capable of accurately determining the temperature of solutions as well as biological systems such as HeLa cancer cells. The nanothermometer is based on the temperature-sensitive fluorescence of NaYF4:Er3+,Yb3+ nanoparticles, where the intensity ratio of the green fluorescence bands of the Er3+ dopant ions (H-2(11/2) -> I-4(15/2) and S-4(3/2) -> I-4(15/2)) changes with temperature. The nanothermometers were first used to obtain thermal profiles created when heating a colloidal solution of NaYF4:Er3+,Yb3+ nanoparticles in water using a pump-probe experiment. Following incubation of the nanoparticles with HeLa cervical cancer cells and their subsequent uptake, the fluorescent nanothermometers measured the internal temperature of the living cell from 25 degrees C to its thermally induced death at 45 degrees C.
publishDate 2010
dc.date.none.fl_str_mv 2010
2010-01-01
2010
2010-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/96493
url https://hdl.handle.net/20.500.14352/96493
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv MAT 2007-64686 Not available Not available
Ministerio de Educación PR2009-0049 ES
CCG087-UAM MAT-4434 Not available
Comunidad de Madrid http://dx.doi.org/10.13039/100012818 S2009 FMAT-1756
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869407334782992384
score 15,300719

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