Analysis of the long-lived responses induced by immunostimulants and their effects on a viral infection in zebrafish (Danio rerio)

16 pages, 7 figures, 2 tables

Detalhes bibliográficos
Autores: Álvarez-Rodríguez, Margarita, Pereiro, Patricia, Reyes-López, Felipe E., Tort, Lluis, Figueras Huerta, Antonio, Novoa, Beatriz
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/307517
Acesso em linha:http://hdl.handle.net/10261/307517
Access Level:acceso abierto
Palavra-chave:Zebrafish
Immunostimulants
β-glucans
Stress
Tolerance
IFN-γ
Kynurenine
TDO
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spelling Analysis of the long-lived responses induced by immunostimulants and their effects on a viral infection in zebrafish (Danio rerio)Álvarez-Rodríguez, MargaritaPereiro, PatriciaReyes-López, Felipe E.Tort, LluisFigueras Huerta, AntonioNovoa, BeatrizZebrafishImmunostimulantsβ-glucansStressToleranceIFN-γKynurenineTDO16 pages, 7 figures, 2 tablesIn recent years, the innate immune response has gained importance since evidence indicates that after an adequate priming protocol, it is possible to obtain some prolonged and enhanced immune responses. Nevertheless, several factors, such as the timing and method of administration of the immunostimulants, must be carefully considered. An inappropriate protocol can transform the treatments into a double-edged sword for the teleost immune system, resulting in a stressful and immunosuppressive state. In this work, we analyzed the long-term effects of different stimuli (β-glucans, lipopolysaccharide, and polyinosinic:polycytidylic acid) on the transcriptome modulation induced by Spring Viremia Carp Virus (SVCV) in adult zebrafish (Danio rerio) and on the mortality caused by this infection. At 35 days post-immunostimulation, the transcriptome was found to be highly altered compared to that of the control fish, and these stimuli also conditioned the response to SVCV challenge, especially in the case of β-glucans. No protection against SVCV was found with any of the stimuli, and non-significant higher mortalities were even observed, especially with β-glucans. However, in the short term (pre-stimulation with β-glucan and infection after 7 days), slight protection was observed after infection. The transcriptome response in the zebrafish kidney at 35 days posttreatment with β-glucans revealed a significant response associated with stress and immunosuppression. The identification of genes that were differentially expressed before and after the infection seemed to indicate a high energy cost of the immunostimulation that was prolonged over time and could explain the lack of protection against SVCV. Differential responses to stress and alterations in lipid metabolism, the tryptophan-kynurenine pathway, and interferon-gamma signaling seem to be some of the mechanisms involved in this response, which represents the end of trained immunity and the beginning of a stressful state characterized by immunosuppression.This work was funded by the projects AGL2014-51773-C3 and BIO2017-82851-C3-1-R from the Spanish Ministerio de Economía y Competitividad, Proyecto Intramural Especial, PIE 201230E057 from Agencia Estatal Consejo Superior de Investigaciones Científicas (CSIC), and IN607B 2016/12 from Consellería de Economía, Emprego e Industria (GAIN), Xunta de Galicia. MA-R was the recipient of an FPU fellowship from the Ministerio de Educación (FPU014/05517).Peer reviewedFrontiers MediaMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas (España)Xunta de GaliciaMinisterio de Educación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/307517reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO//AGL2014-51773-C3-2-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-82851-C3-1-Rhttp://dx.doi.org/10.3389/fimmu.2018.01575Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3075172026-05-22T06:33:51Z
dc.title.none.fl_str_mv Analysis of the long-lived responses induced by immunostimulants and their effects on a viral infection in zebrafish (Danio rerio)
title Analysis of the long-lived responses induced by immunostimulants and their effects on a viral infection in zebrafish (Danio rerio)
spellingShingle Analysis of the long-lived responses induced by immunostimulants and their effects on a viral infection in zebrafish (Danio rerio)
Álvarez-Rodríguez, Margarita
Zebrafish
Immunostimulants
β-glucans
Stress
Tolerance
IFN-γ
Kynurenine
TDO
title_short Analysis of the long-lived responses induced by immunostimulants and their effects on a viral infection in zebrafish (Danio rerio)
title_full Analysis of the long-lived responses induced by immunostimulants and their effects on a viral infection in zebrafish (Danio rerio)
title_fullStr Analysis of the long-lived responses induced by immunostimulants and their effects on a viral infection in zebrafish (Danio rerio)
title_full_unstemmed Analysis of the long-lived responses induced by immunostimulants and their effects on a viral infection in zebrafish (Danio rerio)
title_sort Analysis of the long-lived responses induced by immunostimulants and their effects on a viral infection in zebrafish (Danio rerio)
dc.creator.none.fl_str_mv Álvarez-Rodríguez, Margarita
Pereiro, Patricia
Reyes-López, Felipe E.
Tort, Lluis
Figueras Huerta, Antonio
Novoa, Beatriz
author Álvarez-Rodríguez, Margarita
author_facet Álvarez-Rodríguez, Margarita
Pereiro, Patricia
Reyes-López, Felipe E.
Tort, Lluis
Figueras Huerta, Antonio
Novoa, Beatriz
author_role author
author2 Pereiro, Patricia
Reyes-López, Felipe E.
Tort, Lluis
Figueras Huerta, Antonio
Novoa, Beatriz
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas (España)
Xunta de Galicia
Ministerio de Educación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Zebrafish
Immunostimulants
β-glucans
Stress
Tolerance
IFN-γ
Kynurenine
TDO
topic Zebrafish
Immunostimulants
β-glucans
Stress
Tolerance
IFN-γ
Kynurenine
TDO
description 16 pages, 7 figures, 2 tables
publishDate 2018
dc.date.none.fl_str_mv 2018
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/307517
url http://hdl.handle.net/10261/307517
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO//AGL2014-51773-C3-2-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-82851-C3-1-R
http://dx.doi.org/10.3389/fimmu.2018.01575
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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