Induction of embryogenesis in Brassica napus microspores produces a callosic subintinal layer and abnormal cell walls with altered levels of callose and cellulose

The induction of microspore embryogenesis produces dramatic changes in different aspects of the cell physiology and structure. Changes at the cell wall level are among the most intriguing and poorly understood. In this work, we used high pressure freezing and freeze substitution, immunolocalization,...

ver descrição completa

Detalhes bibliográficos
Autores: Parra Vega, Verónica, Corral Martínez, Patricia, Rivas-Sendra, Alba, Seguí-Simarro, Jose M.|||0000-0001-7672-4169
Formato: artículo
Fecha de publicación:2015
País:España
Recursos:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/73629
Acesso em linha:https://riunet.upv.es/handle/10251/73629
Access Level:acceso abierto
Palavra-chave:Androgenesis
Benzyl alcohol
Caffeine
Doubled haploids
Microspore embryogenesis
Rapeseed
Cell wall carbohydrates.
Electron Microscopy Service of the UPV
GENETICA
id ES_47bcd6044dfab5f50f8682e99e337ab4
oai_identifier_str oai:riunet.upv.es:10251/73629
network_acronym_str ES
network_name_str España
repository_id_str
spelling Induction of embryogenesis in Brassica napus microspores produces a callosic subintinal layer and abnormal cell walls with altered levels of callose and celluloseParra Vega, VerónicaCorral Martínez, PatriciaRivas-Sendra, AlbaSeguí-Simarro, Jose M.|||0000-0001-7672-4169AndrogenesisBenzyl alcoholCaffeineDoubled haploidsMicrospore embryogenesisRapeseedCell wall carbohydrates.Electron Microscopy Service of the UPVGENETICAThe induction of microspore embryogenesis produces dramatic changes in different aspects of the cell physiology and structure. Changes at the cell wall level are among the most intriguing and poorly understood. In this work, we used high pressure freezing and freeze substitution, immunolocalization, confocal and electron microscopy to analyze the structure and composition of the first cell walls formed during conventional Brassica napus microspore embryogenesis, and in cultures treated to alter the intracellular Ca2+ levels. Our results revealed that one of the first signs of embryogenic commitment is the formation of a callose-rich, cellulose-deficient layer beneath the intine (the subintinal layer), and of irregular, incomplete cell walls. In these events, Ca2+ may have a role. We propose that abnormal cell walls are due to a massive callose synthesis and deposition of excreted cytoplasmic material, and the parallel inhibition of cellulose synthesis. These features were absent in pollen-like structures and in microspore-derived embryos, few days after the end of the heat shock, where abnormal cell walls were no longer produced. Together, our results provide an explanation to a series of relevant aspects of microspore embryogenesis including the role of Ca2+ and the occurrence of abnormal cell walls. In addition, our discovery may be the explanation to why nuclear fusions take place during microspore embryogenesis.We want to express our thanks to the staff of the Electron Microscopy Service of Universitat Politecnica de Valencia. Thanks are also due to Dr. Kim Boutilier (PRI Wageningen, The Netherlands) for her help during the stays of VPV and ARS at her lab, to Dr. Samantha Vernhettes (INRA Versailles, France) for her kind gift of S4B staining, and especially to Prof. L. A. Staehelin for his help and friendship during the stay of JMSS at UC Boulder. This work was supported by grants BEST/20081154 from Generalitat Valenciana and AGL2014-55177-R from Spanish MINECO to JMSS.Frontiers MediaDepartamento de BiotecnologíaInstituto Universitario de Conservación y Mejora de la Agrodiversidad ValencianaEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalMinisterio de Economía y CompetitividadGeneralitat ValencianaRepositorio Institucional de la Universitat Politècnica de València Riunet20152015-11-25journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/73629reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengGeneralitat Valenciana https://doi.org/10.13039/501100003359 BEST%2F2008%2F154Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 AGL2014-55177-R NUEVAS VIAS DE MEJORA DE LA EMBRIOGENESIS DE MICROSPORAS EN SOLANACEAS RECALCITRANTES: ESTUDIO DE LA AUTOFAGIA, LA UPR Y LA REGULACION HORMONALopen accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/736292026-06-13T07:49:27Z
dc.title.none.fl_str_mv Induction of embryogenesis in Brassica napus microspores produces a callosic subintinal layer and abnormal cell walls with altered levels of callose and cellulose
title Induction of embryogenesis in Brassica napus microspores produces a callosic subintinal layer and abnormal cell walls with altered levels of callose and cellulose
spellingShingle Induction of embryogenesis in Brassica napus microspores produces a callosic subintinal layer and abnormal cell walls with altered levels of callose and cellulose
Parra Vega, Verónica
Androgenesis
Benzyl alcohol
Caffeine
Doubled haploids
Microspore embryogenesis
Rapeseed
Cell wall carbohydrates.
Electron Microscopy Service of the UPV
GENETICA
title_short Induction of embryogenesis in Brassica napus microspores produces a callosic subintinal layer and abnormal cell walls with altered levels of callose and cellulose
title_full Induction of embryogenesis in Brassica napus microspores produces a callosic subintinal layer and abnormal cell walls with altered levels of callose and cellulose
title_fullStr Induction of embryogenesis in Brassica napus microspores produces a callosic subintinal layer and abnormal cell walls with altered levels of callose and cellulose
title_full_unstemmed Induction of embryogenesis in Brassica napus microspores produces a callosic subintinal layer and abnormal cell walls with altered levels of callose and cellulose
title_sort Induction of embryogenesis in Brassica napus microspores produces a callosic subintinal layer and abnormal cell walls with altered levels of callose and cellulose
dc.creator.none.fl_str_mv Parra Vega, Verónica
Corral Martínez, Patricia
Rivas-Sendra, Alba
Seguí-Simarro, Jose M.|||0000-0001-7672-4169
author Parra Vega, Verónica
author_facet Parra Vega, Verónica
Corral Martínez, Patricia
Rivas-Sendra, Alba
Seguí-Simarro, Jose M.|||0000-0001-7672-4169
author_role author
author2 Corral Martínez, Patricia
Rivas-Sendra, Alba
Seguí-Simarro, Jose M.|||0000-0001-7672-4169
author2_role author
author
author
dc.contributor.none.fl_str_mv Departamento de Biotecnología
Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana
Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
Ministerio de Economía y Competitividad
Generalitat Valenciana
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Androgenesis
Benzyl alcohol
Caffeine
Doubled haploids
Microspore embryogenesis
Rapeseed
Cell wall carbohydrates.
Electron Microscopy Service of the UPV
GENETICA
topic Androgenesis
Benzyl alcohol
Caffeine
Doubled haploids
Microspore embryogenesis
Rapeseed
Cell wall carbohydrates.
Electron Microscopy Service of the UPV
GENETICA
description The induction of microspore embryogenesis produces dramatic changes in different aspects of the cell physiology and structure. Changes at the cell wall level are among the most intriguing and poorly understood. In this work, we used high pressure freezing and freeze substitution, immunolocalization, confocal and electron microscopy to analyze the structure and composition of the first cell walls formed during conventional Brassica napus microspore embryogenesis, and in cultures treated to alter the intracellular Ca2+ levels. Our results revealed that one of the first signs of embryogenic commitment is the formation of a callose-rich, cellulose-deficient layer beneath the intine (the subintinal layer), and of irregular, incomplete cell walls. In these events, Ca2+ may have a role. We propose that abnormal cell walls are due to a massive callose synthesis and deposition of excreted cytoplasmic material, and the parallel inhibition of cellulose synthesis. These features were absent in pollen-like structures and in microspore-derived embryos, few days after the end of the heat shock, where abnormal cell walls were no longer produced. Together, our results provide an explanation to a series of relevant aspects of microspore embryogenesis including the role of Ca2+ and the occurrence of abnormal cell walls. In addition, our discovery may be the explanation to why nuclear fusions take place during microspore embryogenesis.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-11-25
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/73629
url https://riunet.upv.es/handle/10251/73629
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Generalitat Valenciana https://doi.org/10.13039/501100003359 BEST%2F2008%2F154
Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 AGL2014-55177-R NUEVAS VIAS DE MEJORA DE LA EMBRIOGENESIS DE MICROSPORAS EN SOLANACEAS RECALCITRANTES: ESTUDIO DE LA AUTOFAGIA, LA UPR Y LA REGULACION HORMONAL
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869407314510872576
score 15,301603

Registos relacionados