The mesoaccumbens pathway: a retrograde labeling and single-cell axon tracing analysis in the mouse

Neurons in the ventral tegmental area (VTA) that innervate the nucleus accumbens (Acb) constitute the so-called mesoaccumbens system. Increased activity by these neurons is correlated with the expectation and achievement of reward. The mesoaccumbens projection neurons are regarded as a central node...

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Detalles Bibliográficos
Autores: Rodríguez-López, Claudia, Clasca Cabre, Francisco, Prensa Sepúlveda, Lucía
Tipo de recurso: artículo
Fecha de publicación:2007
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/710658
Acceso en línea:http://hdl.handle.net/10486/710658
https://dx.doi.org/10.3389/fnana.2017.00025
Access Level:acceso abierto
Palabra clave:dopamine
accumbens core
accumbens shell
axonal branching
parabraquial pigmented nucleus
paranigral
ventral tegmental area
tyrosine hydroxylase
Medicina
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spelling The mesoaccumbens pathway: a retrograde labeling and single-cell axon tracing analysis in the mouseRodríguez-López, ClaudiaClasca Cabre, FranciscoPrensa Sepúlveda, Lucíadopamineaccumbens coreaccumbens shellaxonal branchingparabraquial pigmented nucleusparanigralventral tegmental areatyrosine hydroxylaseMedicinaNeurons in the ventral tegmental area (VTA) that innervate the nucleus accumbens (Acb) constitute the so-called mesoaccumbens system. Increased activity by these neurons is correlated with the expectation and achievement of reward. The mesoaccumbens projection neurons are regarded as a central node in the brain networks that regulate drive and hedonic experience, and their dysregulation is a common pathophysiological step in addictive behaviors as well as major depression. Despite previous anatomical studies that have analyzed the origin of the mesoaccumbens axons within the VTA, regarded as a unit, the exact contributions of the various cytoarchitectural subdivisions of the VTA to this innervation is still unexplored; understanding these contributions would help further our understanding of their precise anatomical organization. With the aim of deciphering the contribution of the various VTA subdivisions to accumbal innervation, the present study has used retrograde tracer microinjections in the Acb to map the location within the various VTA subdivisions of neurons targeting either the shell or core compartments of the Acb in mice. Furthermore, the dopaminergic nature of these projections has also been analyzed using tyrosine-hydroxylase immunohistochemistry. We demonstrate here that small territories of the Acb core and shell are innervated simultaneously by many VTA subdivisions, contributing dopaminergic as well as non-dopaminergic axons to the accumbal innervation. In fact, single VTA subdivisions harbor both dopaminergic and non-dopaminergic neurons that project to the same accumbal territory. The most medial VTA subnuclei, like the caudal linear nucleus, project abundantly to medial aspects of the Acb core, whereas more lateral territories of the Acb are preferentially targeted by neurons located in the parabrachial pigmented and paranigral nuclei. Overall, about half of the mesoaccumbens neurons are putatively dopaminergic in mice. Anterograde single-cell labeling (Sindbis-pal-eGFP vector) of a limited sample of neurons revealed that mesoaccumbens neurons form profuse terminal arborizations to cover large volumes of either the Acb core or shell, and, unlike other VTA projection neuron populations, they do not branch to other striatal or extrastriatal structures. These anatomical observations are consistent with reports of an intense response in many Acb neurons after stimulation of very few VTA cellsFrontiers MediaDepartamento de Anatomía, Histología y NeurocienciaFacultad de Medicina20072007-03-27research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/710658https://dx.doi.org/10.3389/fnana.2017.00025reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7106582026-06-23T12:46:27Z
dc.title.none.fl_str_mv The mesoaccumbens pathway: a retrograde labeling and single-cell axon tracing analysis in the mouse
title The mesoaccumbens pathway: a retrograde labeling and single-cell axon tracing analysis in the mouse
spellingShingle The mesoaccumbens pathway: a retrograde labeling and single-cell axon tracing analysis in the mouse
Rodríguez-López, Claudia
dopamine
accumbens core
accumbens shell
axonal branching
parabraquial pigmented nucleus
paranigral
ventral tegmental area
tyrosine hydroxylase
Medicina
title_short The mesoaccumbens pathway: a retrograde labeling and single-cell axon tracing analysis in the mouse
title_full The mesoaccumbens pathway: a retrograde labeling and single-cell axon tracing analysis in the mouse
title_fullStr The mesoaccumbens pathway: a retrograde labeling and single-cell axon tracing analysis in the mouse
title_full_unstemmed The mesoaccumbens pathway: a retrograde labeling and single-cell axon tracing analysis in the mouse
title_sort The mesoaccumbens pathway: a retrograde labeling and single-cell axon tracing analysis in the mouse
dc.creator.none.fl_str_mv Rodríguez-López, Claudia
Clasca Cabre, Francisco
Prensa Sepúlveda, Lucía
author Rodríguez-López, Claudia
author_facet Rodríguez-López, Claudia
Clasca Cabre, Francisco
Prensa Sepúlveda, Lucía
author_role author
author2 Clasca Cabre, Francisco
Prensa Sepúlveda, Lucía
author2_role author
author
dc.contributor.none.fl_str_mv Departamento de Anatomía, Histología y Neurociencia
Facultad de Medicina
dc.subject.none.fl_str_mv dopamine
accumbens core
accumbens shell
axonal branching
parabraquial pigmented nucleus
paranigral
ventral tegmental area
tyrosine hydroxylase
Medicina
topic dopamine
accumbens core
accumbens shell
axonal branching
parabraquial pigmented nucleus
paranigral
ventral tegmental area
tyrosine hydroxylase
Medicina
description Neurons in the ventral tegmental area (VTA) that innervate the nucleus accumbens (Acb) constitute the so-called mesoaccumbens system. Increased activity by these neurons is correlated with the expectation and achievement of reward. The mesoaccumbens projection neurons are regarded as a central node in the brain networks that regulate drive and hedonic experience, and their dysregulation is a common pathophysiological step in addictive behaviors as well as major depression. Despite previous anatomical studies that have analyzed the origin of the mesoaccumbens axons within the VTA, regarded as a unit, the exact contributions of the various cytoarchitectural subdivisions of the VTA to this innervation is still unexplored; understanding these contributions would help further our understanding of their precise anatomical organization. With the aim of deciphering the contribution of the various VTA subdivisions to accumbal innervation, the present study has used retrograde tracer microinjections in the Acb to map the location within the various VTA subdivisions of neurons targeting either the shell or core compartments of the Acb in mice. Furthermore, the dopaminergic nature of these projections has also been analyzed using tyrosine-hydroxylase immunohistochemistry. We demonstrate here that small territories of the Acb core and shell are innervated simultaneously by many VTA subdivisions, contributing dopaminergic as well as non-dopaminergic axons to the accumbal innervation. In fact, single VTA subdivisions harbor both dopaminergic and non-dopaminergic neurons that project to the same accumbal territory. The most medial VTA subnuclei, like the caudal linear nucleus, project abundantly to medial aspects of the Acb core, whereas more lateral territories of the Acb are preferentially targeted by neurons located in the parabrachial pigmented and paranigral nuclei. Overall, about half of the mesoaccumbens neurons are putatively dopaminergic in mice. Anterograde single-cell labeling (Sindbis-pal-eGFP vector) of a limited sample of neurons revealed that mesoaccumbens neurons form profuse terminal arborizations to cover large volumes of either the Acb core or shell, and, unlike other VTA projection neuron populations, they do not branch to other striatal or extrastriatal structures. These anatomical observations are consistent with reports of an intense response in many Acb neurons after stimulation of very few VTA cells
publishDate 2007
dc.date.none.fl_str_mv 2007
2007-03-27
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/710658
https://dx.doi.org/10.3389/fnana.2017.00025
url http://hdl.handle.net/10486/710658
https://dx.doi.org/10.3389/fnana.2017.00025
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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