Single-cell profiling of lncRNA expression during Ebola virus infection in rhesus macaques

Long non-coding RNAs (lncRNAs) are involved in numerous biological processes and are pivotal mediators of the immune response, yet little is known about their properties at the single-cell level. Here, we generate a multi-tissue bulk RNAseq dataset from Ebola virus (EBOV) infected and not-infected r...

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Detalles Bibliográficos
Autores: Santus, Luisa, Sopena Rios, Maria, García Pérez, Raquel, 1989-, Lin, Aaron E., Adams, Gordon C., Barnes, Kayla G., Siddle, Katherine J., Wohl, Shirlee, Reverter Comes, Ferran, Rinn, John L., Bennett, Richard S., Hensley, Lisa E., Sabeti, Pardis C., Melé Messeguer, Marta, 1982-
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/58199
Acceso en línea:http://hdl.handle.net/10230/58199
http://dx.doi.org/10.1038/s41467-023-39627-7
Access Level:acceso abierto
Palabra clave:Ebola virus
Gene regulation
Genetics
Rhesus macaque
Transcriptomics
Descripción
Sumario:Long non-coding RNAs (lncRNAs) are involved in numerous biological processes and are pivotal mediators of the immune response, yet little is known about their properties at the single-cell level. Here, we generate a multi-tissue bulk RNAseq dataset from Ebola virus (EBOV) infected and not-infected rhesus macaques and identified 3979 novel lncRNAs. To profile lncRNA expression dynamics in immune circulating single-cells during EBOV infection, we design a metric, Upsilon, to estimate cell-type specificity. Our analysis reveals that lncRNAs are expressed in fewer cells than protein-coding genes, but they are not expressed at lower levels nor are they more cell-type specific when expressed in the same number of cells. In addition, we observe that lncRNAs exhibit similar changes in expression patterns to those of protein-coding genes during EBOV infection, and are often co-expressed with known immune regulators. A few lncRNAs change expression specifically upon EBOV entry in the cell. This study sheds light on the differential features of lncRNAs and protein-coding genes and paves the way for future single-cell lncRNA studies.

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