Hijacking the Hijackers: Escherichia coli Pathogenicity Islands Redirect Helper Phage Packaging for Their Own Benefit

10 páginas, 6 figuras, 2 tablas y material suplementario.

Detalles Bibliográficos
Autores: Fillol-Salom, Alfred, Bacarizo, Julio, Alqasmi, Mohammed, Ciges-Tomas, J. Rafael, Martínez-Rubio, Roser, Roszak, Aleksander W., Cogdell, Richard J., Chen, John, Marina, Alberto, Penadés, José R.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/191827
Acceso en línea:http://hdl.handle.net/10261/191827
Access Level:acceso abierto
Palabra clave:PICI
TerS
Bacteriophage
Evolution
Gene transfer
Pathogenicity islands
Pirating
Transduction
Virulence
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spelling Hijacking the Hijackers: Escherichia coli Pathogenicity Islands Redirect Helper Phage Packaging for Their Own BenefitFillol-Salom, AlfredBacarizo, JulioAlqasmi, MohammedCiges-Tomas, J. RafaelMartínez-Rubio, RoserRoszak, Aleksander W.Cogdell, Richard J.Chen, JohnMarina, AlbertoPenadés, José R.PICITerSBacteriophageEvolutionGene transferPathogenicity islandsPiratingTransductionVirulence10 páginas, 6 figuras, 2 tablas y material suplementario.Phage-inducible chromosomal islands (PICIs) represent a novel and universal class of mobile genetic elements, which have broad impact on bacterial virulence. In spite of their relevance, how the Gram-negative PICIs hijack the phage machinery for their own specific packaging and how they block phage reproduction remains to be determined. Using genetic and structural analyses, we solve the mystery here by showing that the Gram-negative PICIs encode a protein that simultaneously performs these processes. This protein, which we have named Rpp (for redirecting phage packaging), interacts with the phage terminase small subunit, forming a heterocomplex. This complex is unable to recognize the phage DNA, blocking phage packaging, but specifically binds to the PICI genome, promoting PICI packaging. Our studies reveal the mechanism of action that allows PICI dissemination in nature, introducing a new paradigm in the understanding of the biology of pathogenicity islands and therefore of bacterial pathogen evolution.This work was supported by grant BIO2016-78571-P from the Ministerio de Economı´a y Competitividad (Spain) to A.M. and grants MR/M003876/1 and MR/ S00940X/1 from the Medical Research Council (UK), BB/N002873/1 and BB/S003835/1 from the Biotechnology and Biological Sciences Research Council (BBSRC) (UK), Wellcome Trust 201531/Z/16/Z (UK), and ERC-ADG2014 proposal no. 670932 Dut-signal from EU to J.R.P.. X-ray diffraction data collection was supported by Diamond Light Source Block Allocation Group (BAG) proposals MX16258 and MX20229 and by Spanish Synchrotron Radiation Facility ALBA Proposal 2018072901. J.R.P. is thankful to the Royal Society and the Wolfson Foundation for providing him support through a Royal Society Wolfson Fellowship.Peer reviewedElsevierMinisterio de Economía y Competitividad (España)Medical Research Council (UK)Biotechnology and Biological Sciences Research Council (UK)Wolfson FoundationRoyal Society (UK)European Research CouncilMarina, Alberto [0000-0002-1334-5273]Ciges-Tomas, J. Rafael [0000-0003-2647-3052]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/191827reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2016-78571-Pinfo:eu-repo/grantAgreement/EC/FP7/670932http://dx.doi.org/10.1016/j.molcel.2019.06.017Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1918272026-05-22T06:33:51Z
dc.title.none.fl_str_mv Hijacking the Hijackers: Escherichia coli Pathogenicity Islands Redirect Helper Phage Packaging for Their Own Benefit
title Hijacking the Hijackers: Escherichia coli Pathogenicity Islands Redirect Helper Phage Packaging for Their Own Benefit
spellingShingle Hijacking the Hijackers: Escherichia coli Pathogenicity Islands Redirect Helper Phage Packaging for Their Own Benefit
Fillol-Salom, Alfred
PICI
TerS
Bacteriophage
Evolution
Gene transfer
Pathogenicity islands
Pirating
Transduction
Virulence
title_short Hijacking the Hijackers: Escherichia coli Pathogenicity Islands Redirect Helper Phage Packaging for Their Own Benefit
title_full Hijacking the Hijackers: Escherichia coli Pathogenicity Islands Redirect Helper Phage Packaging for Their Own Benefit
title_fullStr Hijacking the Hijackers: Escherichia coli Pathogenicity Islands Redirect Helper Phage Packaging for Their Own Benefit
title_full_unstemmed Hijacking the Hijackers: Escherichia coli Pathogenicity Islands Redirect Helper Phage Packaging for Their Own Benefit
title_sort Hijacking the Hijackers: Escherichia coli Pathogenicity Islands Redirect Helper Phage Packaging for Their Own Benefit
dc.creator.none.fl_str_mv Fillol-Salom, Alfred
Bacarizo, Julio
Alqasmi, Mohammed
Ciges-Tomas, J. Rafael
Martínez-Rubio, Roser
Roszak, Aleksander W.
Cogdell, Richard J.
Chen, John
Marina, Alberto
Penadés, José R.
author Fillol-Salom, Alfred
author_facet Fillol-Salom, Alfred
Bacarizo, Julio
Alqasmi, Mohammed
Ciges-Tomas, J. Rafael
Martínez-Rubio, Roser
Roszak, Aleksander W.
Cogdell, Richard J.
Chen, John
Marina, Alberto
Penadés, José R.
author_role author
author2 Bacarizo, Julio
Alqasmi, Mohammed
Ciges-Tomas, J. Rafael
Martínez-Rubio, Roser
Roszak, Aleksander W.
Cogdell, Richard J.
Chen, John
Marina, Alberto
Penadés, José R.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Medical Research Council (UK)
Biotechnology and Biological Sciences Research Council (UK)
Wolfson Foundation
Royal Society (UK)
European Research Council
Marina, Alberto [0000-0002-1334-5273]
Ciges-Tomas, J. Rafael [0000-0003-2647-3052]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv PICI
TerS
Bacteriophage
Evolution
Gene transfer
Pathogenicity islands
Pirating
Transduction
Virulence
topic PICI
TerS
Bacteriophage
Evolution
Gene transfer
Pathogenicity islands
Pirating
Transduction
Virulence
description 10 páginas, 6 figuras, 2 tablas y material suplementario.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/191827
url http://hdl.handle.net/10261/191827
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2016-78571-P
info:eu-repo/grantAgreement/EC/FP7/670932
http://dx.doi.org/10.1016/j.molcel.2019.06.017
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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