Maturation of biomimetic hydroxyapatite in physiological fluids: a physicochemical and proteomic study

Biomimetic calcium-deficient hydroxyapatite (CDHA) as a bioactive material exhibits exceptional intrinsic osteoinductive and osteogenic properties because of its nanostructure and composition, which promote a favorable microenvironment. Its high reactivity has been hypothesized to play a relevant ro...

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Autores: Konka, J., Espanol, M., Bosch, B. M., Oliveira, Eliandre, Ginebra, M. P.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/182712
Acceso en línea:https://hdl.handle.net/2445/182712
Access Level:acceso abierto
Palabra clave:Materials biomèdics
Nanoestructures
Espectroscòpia Raman
Proteòmica
Biomedical materials
Nanostructures
Raman spectroscopy
Proteomics
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spelling Maturation of biomimetic hydroxyapatite in physiological fluids: a physicochemical and proteomic studyKonka, J.Espanol, M.Bosch, B. M.Oliveira, EliandreGinebra, M. P.Materials biomèdicsNanoestructuresEspectroscòpia RamanProteòmicaBiomedical materialsNanostructuresRaman spectroscopyProteomicsBiomimetic calcium-deficient hydroxyapatite (CDHA) as a bioactive material exhibits exceptional intrinsic osteoinductive and osteogenic properties because of its nanostructure and composition, which promote a favorable microenvironment. Its high reactivity has been hypothesized to play a relevant role in the in vivo performance, mediated by the interaction with the biological fluids, which is amplified by its high specific surface area. Paradoxically, this high reactivity is also behind the in vitro cytotoxicity of this material, especially pro-nounced in static conditions. The present work explores the structural and physicochemical changes that CDHA undergoes in contact with physiological fluids and to investigate its interaction with proteins. Calcium-deficient hydroxyapatite discs with different micro/nanostructures, coarse (C) and fine (F), were exposed to cell-free complete culture medium over extended periods of time: 1, 7, 14, 21, 28, and 50 days. Precipitate formation was not observed in any of the materials in contact with the physiological fluid, which would indicate that the ionic exchanges were linked to incorporation into the crystal structure of CDHA or in the hydrated layer. In fact, CDHA experienced a maturation process, with a progressive increase in crystallinity and the Ca/P ratio, accompanied by an uptake of Mg and a B-type carbonation process, with a gradual propagation into the core of the samples. However, the reactivity of biomimetic hydroxyapatite was highly dependent on the specific surface area and was amplified in nanosized needle-like crystal structures (F), whereas in coarse specimens the ionic exchanges were restricted to the surface, with low penetration in the material bulk. In addition to showing a higher protein adsorption on F substrates, the proteomics study revealed the existence of protein selectivity to-ward F or C microstructures, as well as the capability of CDHA, and more remarkably of F-CDHA, to concentrate specific proteins from the culture medium. Finally, a substantial improvement in the material's ability to support cell proliferation was observed after the CDHA maturation process.2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/182712Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1016/j.mtbio.2021.100137Materials Today Bio, 2021, vol. 12, num. 100137https://doi.org/10.1016/j.mtbio.2021.100137cc by-nc-nd (c) Konka, J. et al., 2021http://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1827122026-05-27T06:46:51Z
dc.title.none.fl_str_mv Maturation of biomimetic hydroxyapatite in physiological fluids: a physicochemical and proteomic study
title Maturation of biomimetic hydroxyapatite in physiological fluids: a physicochemical and proteomic study
spellingShingle Maturation of biomimetic hydroxyapatite in physiological fluids: a physicochemical and proteomic study
Konka, J.
Materials biomèdics
Nanoestructures
Espectroscòpia Raman
Proteòmica
Biomedical materials
Nanostructures
Raman spectroscopy
Proteomics
title_short Maturation of biomimetic hydroxyapatite in physiological fluids: a physicochemical and proteomic study
title_full Maturation of biomimetic hydroxyapatite in physiological fluids: a physicochemical and proteomic study
title_fullStr Maturation of biomimetic hydroxyapatite in physiological fluids: a physicochemical and proteomic study
title_full_unstemmed Maturation of biomimetic hydroxyapatite in physiological fluids: a physicochemical and proteomic study
title_sort Maturation of biomimetic hydroxyapatite in physiological fluids: a physicochemical and proteomic study
dc.creator.none.fl_str_mv Konka, J.
Espanol, M.
Bosch, B. M.
Oliveira, Eliandre
Ginebra, M. P.
author Konka, J.
author_facet Konka, J.
Espanol, M.
Bosch, B. M.
Oliveira, Eliandre
Ginebra, M. P.
author_role author
author2 Espanol, M.
Bosch, B. M.
Oliveira, Eliandre
Ginebra, M. P.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Materials biomèdics
Nanoestructures
Espectroscòpia Raman
Proteòmica
Biomedical materials
Nanostructures
Raman spectroscopy
Proteomics
topic Materials biomèdics
Nanoestructures
Espectroscòpia Raman
Proteòmica
Biomedical materials
Nanostructures
Raman spectroscopy
Proteomics
description Biomimetic calcium-deficient hydroxyapatite (CDHA) as a bioactive material exhibits exceptional intrinsic osteoinductive and osteogenic properties because of its nanostructure and composition, which promote a favorable microenvironment. Its high reactivity has been hypothesized to play a relevant role in the in vivo performance, mediated by the interaction with the biological fluids, which is amplified by its high specific surface area. Paradoxically, this high reactivity is also behind the in vitro cytotoxicity of this material, especially pro-nounced in static conditions. The present work explores the structural and physicochemical changes that CDHA undergoes in contact with physiological fluids and to investigate its interaction with proteins. Calcium-deficient hydroxyapatite discs with different micro/nanostructures, coarse (C) and fine (F), were exposed to cell-free complete culture medium over extended periods of time: 1, 7, 14, 21, 28, and 50 days. Precipitate formation was not observed in any of the materials in contact with the physiological fluid, which would indicate that the ionic exchanges were linked to incorporation into the crystal structure of CDHA or in the hydrated layer. In fact, CDHA experienced a maturation process, with a progressive increase in crystallinity and the Ca/P ratio, accompanied by an uptake of Mg and a B-type carbonation process, with a gradual propagation into the core of the samples. However, the reactivity of biomimetic hydroxyapatite was highly dependent on the specific surface area and was amplified in nanosized needle-like crystal structures (F), whereas in coarse specimens the ionic exchanges were restricted to the surface, with low penetration in the material bulk. In addition to showing a higher protein adsorption on F substrates, the proteomics study revealed the existence of protein selectivity to-ward F or C microstructures, as well as the capability of CDHA, and more remarkably of F-CDHA, to concentrate specific proteins from the culture medium. Finally, a substantial improvement in the material's ability to support cell proliferation was observed after the CDHA maturation process.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/182712
url https://hdl.handle.net/2445/182712
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1016/j.mtbio.2021.100137
Materials Today Bio, 2021, vol. 12, num. 100137
https://doi.org/10.1016/j.mtbio.2021.100137
dc.rights.none.fl_str_mv cc by-nc-nd (c) Konka, J. et al., 2021
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by-nc-nd (c) Konka, J. et al., 2021
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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