The Cucumber vein yellowing virus silencing suppressor P1b can functionally replace HCPro in Plum pox virus infection in a host-specific manner

[EN] Plant viruses of the genera Potyvirus and Ipomovirus (Potyviridae family) use unrelated RNA silencing suppressors (RSS) to counteract antiviral RNA silencing responses. HCPro is the RSS of Potyvirus spp., and its activity is enhanced by the upstream P1 protein. Distinctively, the ipomovirus Cuc...

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Autores: CARBONELL, ALBERTO|||0000-0001-5628-6632, Dujovny, Gabriela, Garcia, Juan Antonio, Valli, Adrian
Tipo de recurso: artículo
Fecha de publicación:2012
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/161157
Acceso en línea:https://riunet.upv.es/handle/10251/161157
Access Level:acceso abierto
Palabra clave:Ppv
RNA silencing
Suppressors
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spelling The Cucumber vein yellowing virus silencing suppressor P1b can functionally replace HCPro in Plum pox virus infection in a host-specific mannerCARBONELL, ALBERTO|||0000-0001-5628-6632Dujovny, GabrielaGarcia, Juan AntonioValli, AdrianPpvRNA silencingSuppressors[EN] Plant viruses of the genera Potyvirus and Ipomovirus (Potyviridae family) use unrelated RNA silencing suppressors (RSS) to counteract antiviral RNA silencing responses. HCPro is the RSS of Potyvirus spp., and its activity is enhanced by the upstream P1 protein. Distinctively, the ipomovirus Cucumber vein yellowing virus (CVYV) lacks HCPro but contains two P1 copies in tandem (P1aP1b), the second of which functions as RSS. Using chimeras based on the potyvirus Plum pox virus (PPV), we found that P1b can functionally replace HCPro in potyviral infections of Nicotiana plants. Interestingly, P1a, the CVYV protein homologous to potyviral P1, disrupted the silencing suppression activity of P1b and reduced the infection efficiency of PPV in Nicotiana benthamiana. Testing the influence of RSS in host specificity, we found that a P1b-expressing chimera poorly infected PPV's natural host, Prunus persica. Conversely, P1b conferred on PPV chimeras the ability to replicate locally in cucumber, CVYV's natural host. The deleterious effect of P1a on PPV infection is host dependent, because the P1aP1b-expressing PPV chimera accumulated in cucumber to higher levels than PPV expressing P1b alone. These results demonstrate that a potyvirus can use different RSS, and that particular RSS and upstream P1-like proteins contribute to defining the virus host range.We thank H. Garcia-Ruiz for critical reading of the manuscript, D. Baulcombe for providing GFP and P19 expression vectors, Rijk Zwaan Iberica (Almeria, Spain) for supplying cucumber seeds, and E. Dominguez for technical assistance. This work was supported by grants BIO2010-18541 from Spanish MICINN, SAL/0185/2006 from Comunidad de Madrid, and KBBE-204429 from European Union. A. Valli was a recipient of an I3P fellowship from CSIC-Fondo Social Europa)Scientific SocietiesInstituto Universitario Mixto de Biología Molecular y Celular de PlantasComunidad de MadridEuropean CommissionMinisterio de Educación y Ciencia e InnovaciónRepositorio Institucional de la Universitat Politècnica de València Riunet20122012-02-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://riunet.upv.es/handle/10251/161157reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengMinisterio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 BIO2010-18541 FACTORES DE LA INTERACCION PLANTA-VIRUS RELEVANTES PARA EL CONTROL DEL VIRUS DE LA SHARKA Y PARA SU USO COMO HERRAMIENTA BIOTECNOLOGICACaja de Ahorros del Mediterráneo https://doi.org/10.13039/100012818 SAL%2F0185%2F2006European Commission https://doi.org/10.13039/501100000780 FP7 204429 Sharka Containmentopen accesshttp://purl.org/coar/access_right/c_abf2Reserva de todos los derechoshttp://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1611572026-06-13T07:49:27Z
dc.title.none.fl_str_mv The Cucumber vein yellowing virus silencing suppressor P1b can functionally replace HCPro in Plum pox virus infection in a host-specific manner
title The Cucumber vein yellowing virus silencing suppressor P1b can functionally replace HCPro in Plum pox virus infection in a host-specific manner
spellingShingle The Cucumber vein yellowing virus silencing suppressor P1b can functionally replace HCPro in Plum pox virus infection in a host-specific manner
CARBONELL, ALBERTO|||0000-0001-5628-6632
Ppv
RNA silencing
Suppressors
title_short The Cucumber vein yellowing virus silencing suppressor P1b can functionally replace HCPro in Plum pox virus infection in a host-specific manner
title_full The Cucumber vein yellowing virus silencing suppressor P1b can functionally replace HCPro in Plum pox virus infection in a host-specific manner
title_fullStr The Cucumber vein yellowing virus silencing suppressor P1b can functionally replace HCPro in Plum pox virus infection in a host-specific manner
title_full_unstemmed The Cucumber vein yellowing virus silencing suppressor P1b can functionally replace HCPro in Plum pox virus infection in a host-specific manner
title_sort The Cucumber vein yellowing virus silencing suppressor P1b can functionally replace HCPro in Plum pox virus infection in a host-specific manner
dc.creator.none.fl_str_mv CARBONELL, ALBERTO|||0000-0001-5628-6632
Dujovny, Gabriela
Garcia, Juan Antonio
Valli, Adrian
author CARBONELL, ALBERTO|||0000-0001-5628-6632
author_facet CARBONELL, ALBERTO|||0000-0001-5628-6632
Dujovny, Gabriela
Garcia, Juan Antonio
Valli, Adrian
author_role author
author2 Dujovny, Gabriela
Garcia, Juan Antonio
Valli, Adrian
author2_role author
author
author
dc.contributor.none.fl_str_mv Instituto Universitario Mixto de Biología Molecular y Celular de Plantas
Comunidad de Madrid
European Commission
Ministerio de Educación y Ciencia e Innovación
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Ppv
RNA silencing
Suppressors
topic Ppv
RNA silencing
Suppressors
description [EN] Plant viruses of the genera Potyvirus and Ipomovirus (Potyviridae family) use unrelated RNA silencing suppressors (RSS) to counteract antiviral RNA silencing responses. HCPro is the RSS of Potyvirus spp., and its activity is enhanced by the upstream P1 protein. Distinctively, the ipomovirus Cucumber vein yellowing virus (CVYV) lacks HCPro but contains two P1 copies in tandem (P1aP1b), the second of which functions as RSS. Using chimeras based on the potyvirus Plum pox virus (PPV), we found that P1b can functionally replace HCPro in potyviral infections of Nicotiana plants. Interestingly, P1a, the CVYV protein homologous to potyviral P1, disrupted the silencing suppression activity of P1b and reduced the infection efficiency of PPV in Nicotiana benthamiana. Testing the influence of RSS in host specificity, we found that a P1b-expressing chimera poorly infected PPV's natural host, Prunus persica. Conversely, P1b conferred on PPV chimeras the ability to replicate locally in cucumber, CVYV's natural host. The deleterious effect of P1a on PPV infection is host dependent, because the P1aP1b-expressing PPV chimera accumulated in cucumber to higher levels than PPV expressing P1b alone. These results demonstrate that a potyvirus can use different RSS, and that particular RSS and upstream P1-like proteins contribute to defining the virus host range.
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-02-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/161157
url https://riunet.upv.es/handle/10251/161157
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 BIO2010-18541 FACTORES DE LA INTERACCION PLANTA-VIRUS RELEVANTES PARA EL CONTROL DEL VIRUS DE LA SHARKA Y PARA SU USO COMO HERRAMIENTA BIOTECNOLOGICA
Caja de Ahorros del Mediterráneo https://doi.org/10.13039/100012818 SAL%2F0185%2F2006
European Commission https://doi.org/10.13039/501100000780 FP7 204429 Sharka Containment
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Scientific Societies
publisher.none.fl_str_mv Scientific Societies
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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