Lung fibrotic tenascin-C upregulation is associated with other extracellular matrix proteins and induced by TGFβ1

Background Idiopathic pulmonary fibrosis (IPF) is a progressive parenchymal lung disease of unknown aetiology and poor prognosis, characterized by altered tissue repair and fibrosis. The extracellular matrix (ECM) is a critical component in regulating cellular homeostasis and appropriate wound heali...

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Autores: Estany, S., Vicens Zygmunt, Vanesa, Llatjós, Roger, Montes Worboys, Ana, Penín, Rosa Maria, Escobar Campuzano, Ignacio, Xaubet Mir, Antonio, Santos Pérez, Salud, Manresa, Federico, Dorca i Sargatal, Jordi, Molina Molina, María
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/67534
Acceso en línea:https://hdl.handle.net/2445/67534
Access Level:acceso abierto
Palabra clave:Fibrosi pulmonar
Matriu extracel·lular
Glicoproteïnes
Pulmonary fibrosis
Extracellular matrix
Glycoproteins
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spelling Lung fibrotic tenascin-C upregulation is associated with other extracellular matrix proteins and induced by TGFβ1Estany, S.Vicens Zygmunt, VanesaLlatjós, RogerMontes Worboys, AnaPenín, Rosa MariaEscobar Campuzano, IgnacioXaubet Mir, AntonioSantos Pérez, SaludManresa, FedericoDorca i Sargatal, JordiMolina Molina, MaríaFibrosi pulmonarMatriu extracel·lularGlicoproteïnesPulmonary fibrosisExtracellular matrixGlycoproteinsBackground Idiopathic pulmonary fibrosis (IPF) is a progressive parenchymal lung disease of unknown aetiology and poor prognosis, characterized by altered tissue repair and fibrosis. The extracellular matrix (ECM) is a critical component in regulating cellular homeostasis and appropriate wound healing. The aim of our study was to determine the expression profile of highlighted ECM proteins in IPF lungs. Methods ECM gene and protein expression was analyzed by cDNA microarrays, rt-PCR, immunohistochemistry and western-blot in lungs from idiopathic pulmonary fibrosis (IPF), hypersensitivity pneumonitis (HP), categorized as chronic (cHP) and subacute (saHP), and healthy lung tissue. Primary fibroblast cultures from normal subjects and fibrotic patients were studied to evaluate tenascin-C (TNC) synthesis. Results A total of 20 ECM proteins were upregulated and 6 proteins downregulated in IPF. TNC was almost undetected in normal lungs and significantly upregulated in fibrotic lungs (IPF and cHP) compared to saHP. Furthermore, it was located specifically in the fibroblastic foci areas of the fibrotic lung with a subepithelial gradient pattern. TNC levels were correlated with fibroblastic foci content in cHP lungs. Versican and fibronectin glycoproteins were associated with TNC, mainly in fibroblastic foci of fibrotic lungs. Fibroblasts from IPF patients constitutively synthesized higher levels of TNC than normal fibroblasts. TNC and α-sma was induced by TGF-β1 in both fibrotic and normal fibroblasts. TNC treatment of normal and fibrotic fibroblasts induced a non-significant increased α-sma mRNA. Conclusions The difference in ECM glycoprotein content in interstitial lung diseases could contribute to the development of lung fibrosis. The increase of TNC in interstitial areas of fibrotic activity could play a key role in the altered wound healing.BioMed Central2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/67534Articles publicats en revistes (Ciències Clíniques)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: http://dx.doi.org/10.1186/1471-2466-14-120BMC Pulmonary Medicine, 2014, vol. 14, p. 120http://dx.doi.org/10.1186/1471-2466-14-120cc-by (c) Estany, S. et al., 2014http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/675342026-05-27T06:46:51Z
dc.title.none.fl_str_mv Lung fibrotic tenascin-C upregulation is associated with other extracellular matrix proteins and induced by TGFβ1
title Lung fibrotic tenascin-C upregulation is associated with other extracellular matrix proteins and induced by TGFβ1
spellingShingle Lung fibrotic tenascin-C upregulation is associated with other extracellular matrix proteins and induced by TGFβ1
Estany, S.
Fibrosi pulmonar
Matriu extracel·lular
Glicoproteïnes
Pulmonary fibrosis
Extracellular matrix
Glycoproteins
title_short Lung fibrotic tenascin-C upregulation is associated with other extracellular matrix proteins and induced by TGFβ1
title_full Lung fibrotic tenascin-C upregulation is associated with other extracellular matrix proteins and induced by TGFβ1
title_fullStr Lung fibrotic tenascin-C upregulation is associated with other extracellular matrix proteins and induced by TGFβ1
title_full_unstemmed Lung fibrotic tenascin-C upregulation is associated with other extracellular matrix proteins and induced by TGFβ1
title_sort Lung fibrotic tenascin-C upregulation is associated with other extracellular matrix proteins and induced by TGFβ1
dc.creator.none.fl_str_mv Estany, S.
Vicens Zygmunt, Vanesa
Llatjós, Roger
Montes Worboys, Ana
Penín, Rosa Maria
Escobar Campuzano, Ignacio
Xaubet Mir, Antonio
Santos Pérez, Salud
Manresa, Federico
Dorca i Sargatal, Jordi
Molina Molina, María
author Estany, S.
author_facet Estany, S.
Vicens Zygmunt, Vanesa
Llatjós, Roger
Montes Worboys, Ana
Penín, Rosa Maria
Escobar Campuzano, Ignacio
Xaubet Mir, Antonio
Santos Pérez, Salud
Manresa, Federico
Dorca i Sargatal, Jordi
Molina Molina, María
author_role author
author2 Vicens Zygmunt, Vanesa
Llatjós, Roger
Montes Worboys, Ana
Penín, Rosa Maria
Escobar Campuzano, Ignacio
Xaubet Mir, Antonio
Santos Pérez, Salud
Manresa, Federico
Dorca i Sargatal, Jordi
Molina Molina, María
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Fibrosi pulmonar
Matriu extracel·lular
Glicoproteïnes
Pulmonary fibrosis
Extracellular matrix
Glycoproteins
topic Fibrosi pulmonar
Matriu extracel·lular
Glicoproteïnes
Pulmonary fibrosis
Extracellular matrix
Glycoproteins
description Background Idiopathic pulmonary fibrosis (IPF) is a progressive parenchymal lung disease of unknown aetiology and poor prognosis, characterized by altered tissue repair and fibrosis. The extracellular matrix (ECM) is a critical component in regulating cellular homeostasis and appropriate wound healing. The aim of our study was to determine the expression profile of highlighted ECM proteins in IPF lungs. Methods ECM gene and protein expression was analyzed by cDNA microarrays, rt-PCR, immunohistochemistry and western-blot in lungs from idiopathic pulmonary fibrosis (IPF), hypersensitivity pneumonitis (HP), categorized as chronic (cHP) and subacute (saHP), and healthy lung tissue. Primary fibroblast cultures from normal subjects and fibrotic patients were studied to evaluate tenascin-C (TNC) synthesis. Results A total of 20 ECM proteins were upregulated and 6 proteins downregulated in IPF. TNC was almost undetected in normal lungs and significantly upregulated in fibrotic lungs (IPF and cHP) compared to saHP. Furthermore, it was located specifically in the fibroblastic foci areas of the fibrotic lung with a subepithelial gradient pattern. TNC levels were correlated with fibroblastic foci content in cHP lungs. Versican and fibronectin glycoproteins were associated with TNC, mainly in fibroblastic foci of fibrotic lungs. Fibroblasts from IPF patients constitutively synthesized higher levels of TNC than normal fibroblasts. TNC and α-sma was induced by TGF-β1 in both fibrotic and normal fibroblasts. TNC treatment of normal and fibrotic fibroblasts induced a non-significant increased α-sma mRNA. Conclusions The difference in ECM glycoprotein content in interstitial lung diseases could contribute to the development of lung fibrosis. The increase of TNC in interstitial areas of fibrotic activity could play a key role in the altered wound healing.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/67534
url https://hdl.handle.net/2445/67534
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1186/1471-2466-14-120
BMC Pulmonary Medicine, 2014, vol. 14, p. 120
http://dx.doi.org/10.1186/1471-2466-14-120
dc.rights.none.fl_str_mv cc-by (c) Estany, S. et al., 2014
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Estany, S. et al., 2014
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv Articles publicats en revistes (Ciències Clíniques)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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