Patterned Biolayers of Protein Antigens for Label-Free Biosensing in Cow Milk Allergy

[EN] This paper focuses on creating one-dimensional diffractive grooved structures of antigen proteins on glass substrates for the label-free detection of antibodies to dairy allergens. In particular, the fabrication of protein structures is carried out by combining microcontact printing with physis...

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Autores: Juste-Dolz, Augusto Miguel|||0000-0003-4889-6419, Puchades, Rosa|||0000-0002-9329-1593, Avella-Oliver, Miquel|||0000-0002-7293-6989, Maquieira, Angel|||0000-0003-4641-4957, Fernández-Sánchez, María Estrella
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/202267
Acceso en línea:https://riunet.upv.es/handle/10251/202267
Access Level:acceso abierto
Palabra clave:Diffraction
Grating
Microcontact printing
Casein
Bovine serum albumin
SS-lactoglobulin
Covalent
Immunoglobulin G
Dairy
Beef
QUIMICA ANALITICA
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network_acronym_str ES
network_name_str España
repository_id_str
spelling Patterned Biolayers of Protein Antigens for Label-Free Biosensing in Cow Milk AllergyJuste-Dolz, Augusto Miguel|||0000-0003-4889-6419Puchades, Rosa|||0000-0002-9329-1593Avella-Oliver, Miquel|||0000-0002-7293-6989Maquieira, Angel|||0000-0003-4641-4957Fernández-Sánchez, María EstrellaDiffractionGratingMicrocontact printingCaseinBovine serum albuminSS-lactoglobulinCovalentImmunoglobulin GDairyBeefQUIMICA ANALITICA[EN] This paper focuses on creating one-dimensional diffractive grooved structures of antigen proteins on glass substrates for the label-free detection of antibodies to dairy allergens. In particular, the fabrication of protein structures is carried out by combining microcontact printing with physisorption, imines coupling, and thiol-ene click chemistry. The work first sets up these patterning methods and discusses and compares the main aspects involved in them (structure, biolayer thickness, functionality, stability). Homogeneous periodic submicron structures of proteins are created and characterized by diffractive measurements, AFM, FESEM, and fluorescence scanning. Then, this patterning method is applied to proteins involved in cow milk allergy, and the resulting structures are implemented as optical transducers to sense specific immunoglobulins G. In particular, gratings of bovine serum albumin, casein, and ß-lactoglobulin are created and assessed, reaching limits of detection in the range of 30¿45 ng·mL¿1 of unlabeled antibodies by diffractive biosensing.Grant PID2019-110713RB-I00 funded by MCIN/AEI/10.13039/501100011033 and cofunded by "ERDF A way of making Europe", Generalitat Valenciana (PROMETEO/2020/094), and Universitat Politecnica de Valencia (PAID-06-22).MDPI AGEscuela Técnica Superior de Ingeniería Aeroespacial y Diseño IndustrialDepartamento de QuímicaInstituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo TecnológicoEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalCentro de Investigación e Innovación en BioingenieríaGeneralitat ValencianaAgencia Estatal de InvestigaciónEuropean Regional Development FundUniversitat Politècnica de ValènciaRepositorio Institucional de la Universitat Politècnica de València Riunet20232023-02-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/202267reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2019-110713RB-I00 HACIA EL BIOSENSADO HOLOGRAFICO AVANZADOUniversitat Politècnica de València https://doi.org/10.13039/501100004233 PAID-06-22 Erradicación del riesgo de obstrucción de drenajes por arrastre de mobiliario urbano y vehículos por inundación (ERODAMUVI)Generalitat Valenciana https://doi.org/10.13039/501100003359 PROMETEO%2F2020%2F094 HOLOGRAFÍA. UNA VIA PARA AFRONTAR NUEVOS RETOS EN BIOSENSADOopen accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/2022672026-06-13T07:49:27Z
dc.title.none.fl_str_mv Patterned Biolayers of Protein Antigens for Label-Free Biosensing in Cow Milk Allergy
title Patterned Biolayers of Protein Antigens for Label-Free Biosensing in Cow Milk Allergy
spellingShingle Patterned Biolayers of Protein Antigens for Label-Free Biosensing in Cow Milk Allergy
Juste-Dolz, Augusto Miguel|||0000-0003-4889-6419
Diffraction
Grating
Microcontact printing
Casein
Bovine serum albumin
SS-lactoglobulin
Covalent
Immunoglobulin G
Dairy
Beef
QUIMICA ANALITICA
title_short Patterned Biolayers of Protein Antigens for Label-Free Biosensing in Cow Milk Allergy
title_full Patterned Biolayers of Protein Antigens for Label-Free Biosensing in Cow Milk Allergy
title_fullStr Patterned Biolayers of Protein Antigens for Label-Free Biosensing in Cow Milk Allergy
title_full_unstemmed Patterned Biolayers of Protein Antigens for Label-Free Biosensing in Cow Milk Allergy
title_sort Patterned Biolayers of Protein Antigens for Label-Free Biosensing in Cow Milk Allergy
dc.creator.none.fl_str_mv Juste-Dolz, Augusto Miguel|||0000-0003-4889-6419
Puchades, Rosa|||0000-0002-9329-1593
Avella-Oliver, Miquel|||0000-0002-7293-6989
Maquieira, Angel|||0000-0003-4641-4957
Fernández-Sánchez, María Estrella
author Juste-Dolz, Augusto Miguel|||0000-0003-4889-6419
author_facet Juste-Dolz, Augusto Miguel|||0000-0003-4889-6419
Puchades, Rosa|||0000-0002-9329-1593
Avella-Oliver, Miquel|||0000-0002-7293-6989
Maquieira, Angel|||0000-0003-4641-4957
Fernández-Sánchez, María Estrella
author_role author
author2 Puchades, Rosa|||0000-0002-9329-1593
Avella-Oliver, Miquel|||0000-0002-7293-6989
Maquieira, Angel|||0000-0003-4641-4957
Fernández-Sánchez, María Estrella
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Escuela Técnica Superior de Ingeniería Aeroespacial y Diseño Industrial
Departamento de Química
Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico
Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
Centro de Investigación e Innovación en Bioingeniería
Generalitat Valenciana
Agencia Estatal de Investigación
European Regional Development Fund
Universitat Politècnica de València
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Diffraction
Grating
Microcontact printing
Casein
Bovine serum albumin
SS-lactoglobulin
Covalent
Immunoglobulin G
Dairy
Beef
QUIMICA ANALITICA
topic Diffraction
Grating
Microcontact printing
Casein
Bovine serum albumin
SS-lactoglobulin
Covalent
Immunoglobulin G
Dairy
Beef
QUIMICA ANALITICA
description [EN] This paper focuses on creating one-dimensional diffractive grooved structures of antigen proteins on glass substrates for the label-free detection of antibodies to dairy allergens. In particular, the fabrication of protein structures is carried out by combining microcontact printing with physisorption, imines coupling, and thiol-ene click chemistry. The work first sets up these patterning methods and discusses and compares the main aspects involved in them (structure, biolayer thickness, functionality, stability). Homogeneous periodic submicron structures of proteins are created and characterized by diffractive measurements, AFM, FESEM, and fluorescence scanning. Then, this patterning method is applied to proteins involved in cow milk allergy, and the resulting structures are implemented as optical transducers to sense specific immunoglobulins G. In particular, gratings of bovine serum albumin, casein, and ß-lactoglobulin are created and assessed, reaching limits of detection in the range of 30¿45 ng·mL¿1 of unlabeled antibodies by diffractive biosensing.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-02-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/202267
url https://riunet.upv.es/handle/10251/202267
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2019-110713RB-I00 HACIA EL BIOSENSADO HOLOGRAFICO AVANZADO
Universitat Politècnica de València https://doi.org/10.13039/501100004233 PAID-06-22 Erradicación del riesgo de obstrucción de drenajes por arrastre de mobiliario urbano y vehículos por inundación (ERODAMUVI)
Generalitat Valenciana https://doi.org/10.13039/501100003359 PROMETEO%2F2020%2F094 HOLOGRAFÍA. UNA VIA PARA AFRONTAR NUEVOS RETOS EN BIOSENSADO
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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