3D bioprinted functional skeletal muscle models have potential applications for studies of muscle wasting in cancer cachexia

Acquired muscle diseases such as cancer cachexia are responsible for the poor prognosis of many patients suffering from cancer. In vitro models are needed to study the underlying mechanisms of those pathologies. Extrusion bioprinting is an emerging tool to emulate the aligned architecture of fibers...

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Autores: García Lizarribar, Andrea, Villasante, Aranzazu, Lopez Martin, Jose Antonio, Flandez, Marta, Soler Vázquez, M. Carmen, Serra i Cucurull, Dolors, Herrero Rodríguez, Laura, Sagrera, Ana, Efeyan, Alejo, Samitier i Martí, Josep
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Recursos:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/200900
Acesso em linha:https://hdl.handle.net/2445/200900
Access Level:acceso abierto
Palavra-chave:Enginyeria de teixits
Caquèxia
Materials biomèdics
Malalties musculars
Tissue engineering
Cachexia
Biomedical materials
Muscular Diseases
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spelling 3D bioprinted functional skeletal muscle models have potential applications for studies of muscle wasting in cancer cachexiaGarcía Lizarribar, AndreaVillasante, AranzazuLopez Martin, Jose AntonioFlandez, MartaSoler Vázquez, M. CarmenSerra i Cucurull, DolorsHerrero Rodríguez, LauraSagrera, AnaEfeyan, AlejoSamitier i Martí, JosepEnginyeria de teixitsCaquèxiaMaterials biomèdicsMalalties muscularsTissue engineeringCachexiaBiomedical materialsMuscular DiseasesAcquired muscle diseases such as cancer cachexia are responsible for the poor prognosis of many patients suffering from cancer. In vitro models are needed to study the underlying mechanisms of those pathologies. Extrusion bioprinting is an emerging tool to emulate the aligned architecture of fibers while implementing ad- ditive manufacturing techniques in tissue engineering. However, designing bioinks that reconcile the rheological needs of bioprinting and the biological requirements of muscle tissue is a challenging matter. Here we formulate a biomaterial with dual crosslinking to modulate the physical properties of bioprinted models. We design 3D bioprinted muscle models that resemble the mechanical properties of native tissue and show improved prolif- eration and high maturation of differentiated myotubes suggesting that the GelMA-AlgMA-Fibrin biomaterial possesses myogenic properties. The electrical stimulation of the 3D model confirmed the contractile capability of the tissue and enhanced the formation of sarcomeres. Regarding the functionality of the models, they served as platforms to recapitulate skeletal muscle diseases such as muscle wasting produced by cancer cachexia. The genetic expression of 3D models demonstrated a better resemblance to the muscular biopsies of cachectic mouse models. Altogether, this biomaterial is aimed to fabricate manipulable skeletal muscle in vitro models in a non- costly, fast and feasible mannerElsevier B.V.2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/200900Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1016/j.bioadv.2023.213426Biomaterials Advances, 2023, vol. 150, p. 213426https://doi.org/10.1016/j.bioadv.2023.213426cc-by-nc-nd (c) García Lizarribar, Andrea et al., 2023https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/2009002026-05-27T06:46:51Z
dc.title.none.fl_str_mv 3D bioprinted functional skeletal muscle models have potential applications for studies of muscle wasting in cancer cachexia
title 3D bioprinted functional skeletal muscle models have potential applications for studies of muscle wasting in cancer cachexia
spellingShingle 3D bioprinted functional skeletal muscle models have potential applications for studies of muscle wasting in cancer cachexia
García Lizarribar, Andrea
Enginyeria de teixits
Caquèxia
Materials biomèdics
Malalties musculars
Tissue engineering
Cachexia
Biomedical materials
Muscular Diseases
title_short 3D bioprinted functional skeletal muscle models have potential applications for studies of muscle wasting in cancer cachexia
title_full 3D bioprinted functional skeletal muscle models have potential applications for studies of muscle wasting in cancer cachexia
title_fullStr 3D bioprinted functional skeletal muscle models have potential applications for studies of muscle wasting in cancer cachexia
title_full_unstemmed 3D bioprinted functional skeletal muscle models have potential applications for studies of muscle wasting in cancer cachexia
title_sort 3D bioprinted functional skeletal muscle models have potential applications for studies of muscle wasting in cancer cachexia
dc.creator.none.fl_str_mv García Lizarribar, Andrea
Villasante, Aranzazu
Lopez Martin, Jose Antonio
Flandez, Marta
Soler Vázquez, M. Carmen
Serra i Cucurull, Dolors
Herrero Rodríguez, Laura
Sagrera, Ana
Efeyan, Alejo
Samitier i Martí, Josep
author García Lizarribar, Andrea
author_facet García Lizarribar, Andrea
Villasante, Aranzazu
Lopez Martin, Jose Antonio
Flandez, Marta
Soler Vázquez, M. Carmen
Serra i Cucurull, Dolors
Herrero Rodríguez, Laura
Sagrera, Ana
Efeyan, Alejo
Samitier i Martí, Josep
author_role author
author2 Villasante, Aranzazu
Lopez Martin, Jose Antonio
Flandez, Marta
Soler Vázquez, M. Carmen
Serra i Cucurull, Dolors
Herrero Rodríguez, Laura
Sagrera, Ana
Efeyan, Alejo
Samitier i Martí, Josep
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Enginyeria de teixits
Caquèxia
Materials biomèdics
Malalties musculars
Tissue engineering
Cachexia
Biomedical materials
Muscular Diseases
topic Enginyeria de teixits
Caquèxia
Materials biomèdics
Malalties musculars
Tissue engineering
Cachexia
Biomedical materials
Muscular Diseases
description Acquired muscle diseases such as cancer cachexia are responsible for the poor prognosis of many patients suffering from cancer. In vitro models are needed to study the underlying mechanisms of those pathologies. Extrusion bioprinting is an emerging tool to emulate the aligned architecture of fibers while implementing ad- ditive manufacturing techniques in tissue engineering. However, designing bioinks that reconcile the rheological needs of bioprinting and the biological requirements of muscle tissue is a challenging matter. Here we formulate a biomaterial with dual crosslinking to modulate the physical properties of bioprinted models. We design 3D bioprinted muscle models that resemble the mechanical properties of native tissue and show improved prolif- eration and high maturation of differentiated myotubes suggesting that the GelMA-AlgMA-Fibrin biomaterial possesses myogenic properties. The electrical stimulation of the 3D model confirmed the contractile capability of the tissue and enhanced the formation of sarcomeres. Regarding the functionality of the models, they served as platforms to recapitulate skeletal muscle diseases such as muscle wasting produced by cancer cachexia. The genetic expression of 3D models demonstrated a better resemblance to the muscular biopsies of cachectic mouse models. Altogether, this biomaterial is aimed to fabricate manipulable skeletal muscle in vitro models in a non- costly, fast and feasible manner
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/200900
url https://hdl.handle.net/2445/200900
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1016/j.bioadv.2023.213426
Biomaterials Advances, 2023, vol. 150, p. 213426
https://doi.org/10.1016/j.bioadv.2023.213426
dc.rights.none.fl_str_mv cc-by-nc-nd (c) García Lizarribar, Andrea et al., 2023
https://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) García Lizarribar, Andrea et al., 2023
https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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