Measurement of Calprotectin (S100A8/A9) in the Saliva of Pigs

Calprotectin (CALP, S100A8/A9) is a calcium and zinc-binding protein involved in inflammation that has a wide range of proinflammatory functions, such as cytokine production and regulation of leukocyte adhesion, migration, and phagocytosis. The objective of this study was to validate a commercially...

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Authors: López-Martínez, María José|||0000-0003-4159-037X, Martínez-Subiela, Silvia|||0000-0002-1524-1558, Cerón, José Joaquín|||0000-0002-8654-1793, Ortín-Bustillo, Alba, Ramis, Guillermo, Lopez Arjona, Marina|||0000-0003-3025-440X, Martínez-Miró, Silvia|||0000-0002-1350-0886, García Manzanilla, Edgar|||0000-0002-2301-2560, Eckersall, Peter David|||0000-0001-5254-5502, Tecles, Fernando|||0000-0002-7635-533X, Escribano Tortosa, Damián|||0000-0001-9439-6779, Muñoz Prieto, Alberto|||0000-0001-6865-8712
Format: article
Publication Date:2023
Country:España
Institution:Universitat Autònoma de Barcelona
Repository:Dipòsit Digital de Documents de la UAB
Language:English
OAI Identifier:oai:ddd.uab.cat:275290
Online Access:https://ddd.uab.cat/record/275290
https://dx.doi.org/urn:doi:10.3390/ani13071190
Access Level:Open access
Keyword:S100A8/A9
Calprotectin
Automated assay
Pig
Saliva
Stress
Sepsis
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spelling Measurement of Calprotectin (S100A8/A9) in the Saliva of PigsValidation Data of A Commercially Available Automated Assay and Changes in Sepsis, Inflammation, and StressLópez-Martínez, María José|||0000-0003-4159-037XMartínez-Subiela, Silvia|||0000-0002-1524-1558Cerón, José Joaquín|||0000-0002-8654-1793Ortín-Bustillo, AlbaRamis, GuillermoLopez Arjona, Marina|||0000-0003-3025-440XMartínez-Miró, Silvia|||0000-0002-1350-0886García Manzanilla, Edgar|||0000-0002-2301-2560Eckersall, Peter David|||0000-0001-5254-5502Tecles, Fernando|||0000-0002-7635-533XEscribano Tortosa, Damián|||0000-0001-9439-6779Muñoz Prieto, Alberto|||0000-0001-6865-8712S100A8/A9CalprotectinAutomated assayPigSalivaStressSepsisCalprotectin (CALP, S100A8/A9) is a calcium and zinc-binding protein involved in inflammation that has a wide range of proinflammatory functions, such as cytokine production and regulation of leukocyte adhesion, migration, and phagocytosis. The objective of this study was to validate a commercially available assay for the measurement of CALP in the saliva of pigs and study the variations of this analyte due to sepsis, non-septic inflammation, and stress. The assay showed adequate precision and accuracy for the measurements of CALP in the saliva of pigs. In addition, this protein showed significant increases in the saliva of pigs with sepsis as well as after a stressful situation in our experimental conditions, being the increase in the stress of lower magnitude than in sepsis. Based on these results, CALP can be measured in the saliva of pigs and could be a potential biomarker of health and welfare in this species. Calprotectin (CALP, S100A8/A9), also named myeloid-related protein 8/14, is a dimer complex of S100A8 and S100A9 that belongs to the S-100 protein family. It is involved in inflammation and has a wide range of proinflammatory functions, such as cytokine production and regulation of leukocyte adhesion, migration, and phagocytosis. In humans, CALP traditionally can be measured in faeces, serum, and saliva as a biomarker of inflammation and sepsis. The objective of this study was to validate an automated assay for CALP measurements in the saliva of pigs, having the advantage of the use of a non-invasive sample that is easy to collect. The assay was precise and accurate. CALP in saliva measured by this assay showed significant changes depending on the hour of the day. It also showed significant increases in the saliva of pigs after the administration of lipopolysaccharide (LPS), and showed a rise, although with increases of lower magnitude, after a stressful stimulus. Further studies should be made to gain knowledge about the possible practical applications of the measurements of CALP in the saliva of pigs as a biomarker to evaluate the animals' health and welfare. 22023-01-0120232023-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/275290https://dx.doi.org/urn:doi:10.3390/ani13071190reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Ciencia e Innovación https://doi.org/10.13039/501100004837 PID2019-105950RB-100Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PCI2020-120712-2Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 FJC2021-047105-IMinisterio de Ciencia e Innovación https://doi.org/10.13039/501100004837 RYC2021-033660-Iopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2752902026-06-06T12:50:31Z
dc.title.none.fl_str_mv Measurement of Calprotectin (S100A8/A9) in the Saliva of Pigs
Validation Data of A Commercially Available Automated Assay and Changes in Sepsis, Inflammation, and Stress
title Measurement of Calprotectin (S100A8/A9) in the Saliva of Pigs
spellingShingle Measurement of Calprotectin (S100A8/A9) in the Saliva of Pigs
López-Martínez, María José|||0000-0003-4159-037X
S100A8/A9
Calprotectin
Automated assay
Pig
Saliva
Stress
Sepsis
title_short Measurement of Calprotectin (S100A8/A9) in the Saliva of Pigs
title_full Measurement of Calprotectin (S100A8/A9) in the Saliva of Pigs
title_fullStr Measurement of Calprotectin (S100A8/A9) in the Saliva of Pigs
title_full_unstemmed Measurement of Calprotectin (S100A8/A9) in the Saliva of Pigs
title_sort Measurement of Calprotectin (S100A8/A9) in the Saliva of Pigs
dc.creator.none.fl_str_mv López-Martínez, María José|||0000-0003-4159-037X
Martínez-Subiela, Silvia|||0000-0002-1524-1558
Cerón, José Joaquín|||0000-0002-8654-1793
Ortín-Bustillo, Alba
Ramis, Guillermo
Lopez Arjona, Marina|||0000-0003-3025-440X
Martínez-Miró, Silvia|||0000-0002-1350-0886
García Manzanilla, Edgar|||0000-0002-2301-2560
Eckersall, Peter David|||0000-0001-5254-5502
Tecles, Fernando|||0000-0002-7635-533X
Escribano Tortosa, Damián|||0000-0001-9439-6779
Muñoz Prieto, Alberto|||0000-0001-6865-8712
author López-Martínez, María José|||0000-0003-4159-037X
author_facet López-Martínez, María José|||0000-0003-4159-037X
Martínez-Subiela, Silvia|||0000-0002-1524-1558
Cerón, José Joaquín|||0000-0002-8654-1793
Ortín-Bustillo, Alba
Ramis, Guillermo
Lopez Arjona, Marina|||0000-0003-3025-440X
Martínez-Miró, Silvia|||0000-0002-1350-0886
García Manzanilla, Edgar|||0000-0002-2301-2560
Eckersall, Peter David|||0000-0001-5254-5502
Tecles, Fernando|||0000-0002-7635-533X
Escribano Tortosa, Damián|||0000-0001-9439-6779
Muñoz Prieto, Alberto|||0000-0001-6865-8712
author_role author
author2 Martínez-Subiela, Silvia|||0000-0002-1524-1558
Cerón, José Joaquín|||0000-0002-8654-1793
Ortín-Bustillo, Alba
Ramis, Guillermo
Lopez Arjona, Marina|||0000-0003-3025-440X
Martínez-Miró, Silvia|||0000-0002-1350-0886
García Manzanilla, Edgar|||0000-0002-2301-2560
Eckersall, Peter David|||0000-0001-5254-5502
Tecles, Fernando|||0000-0002-7635-533X
Escribano Tortosa, Damián|||0000-0001-9439-6779
Muñoz Prieto, Alberto|||0000-0001-6865-8712
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv S100A8/A9
Calprotectin
Automated assay
Pig
Saliva
Stress
Sepsis
topic S100A8/A9
Calprotectin
Automated assay
Pig
Saliva
Stress
Sepsis
description Calprotectin (CALP, S100A8/A9) is a calcium and zinc-binding protein involved in inflammation that has a wide range of proinflammatory functions, such as cytokine production and regulation of leukocyte adhesion, migration, and phagocytosis. The objective of this study was to validate a commercially available assay for the measurement of CALP in the saliva of pigs and study the variations of this analyte due to sepsis, non-septic inflammation, and stress. The assay showed adequate precision and accuracy for the measurements of CALP in the saliva of pigs. In addition, this protein showed significant increases in the saliva of pigs with sepsis as well as after a stressful situation in our experimental conditions, being the increase in the stress of lower magnitude than in sepsis. Based on these results, CALP can be measured in the saliva of pigs and could be a potential biomarker of health and welfare in this species. Calprotectin (CALP, S100A8/A9), also named myeloid-related protein 8/14, is a dimer complex of S100A8 and S100A9 that belongs to the S-100 protein family. It is involved in inflammation and has a wide range of proinflammatory functions, such as cytokine production and regulation of leukocyte adhesion, migration, and phagocytosis. In humans, CALP traditionally can be measured in faeces, serum, and saliva as a biomarker of inflammation and sepsis. The objective of this study was to validate an automated assay for CALP measurements in the saliva of pigs, having the advantage of the use of a non-invasive sample that is easy to collect. The assay was precise and accurate. CALP in saliva measured by this assay showed significant changes depending on the hour of the day. It also showed significant increases in the saliva of pigs after the administration of lipopolysaccharide (LPS), and showed a rise, although with increases of lower magnitude, after a stressful stimulus. Further studies should be made to gain knowledge about the possible practical applications of the measurements of CALP in the saliva of pigs as a biomarker to evaluate the animals' health and welfare.
publishDate 2023
dc.date.none.fl_str_mv 2
2023-01-01
2023
2023-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/275290
https://dx.doi.org/urn:doi:10.3390/ani13071190
url https://ddd.uab.cat/record/275290
https://dx.doi.org/urn:doi:10.3390/ani13071190
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 PID2019-105950RB-100
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PCI2020-120712-2
Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 FJC2021-047105-I
Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 RYC2021-033660-I
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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