Tissue-Specific Signatures in the Transcriptional Response to Anaplasma phagocytophilum Infection of Ixodes scapularis and Ixodes ricinus Tick Cell Lines

12 páginas, 1 tabla, 7 figuras. -- This document is protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permission

Detalles Bibliográficos
Autores: Alberdi, Pilar, Mansfield, Karen L., Manzano Román, Raúl, Cook, Charlotte, Ayllón, Nieves, Villar, Margarita, Johnson, Nicholas, Fooks, Anthony R., Fuente, José de la
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/129828
Acceso en línea:http://hdl.handle.net/10261/129828
Access Level:acceso abierto
Palabra clave:Transcriptomics
Tick
Rickettsia
Anaplasma
Apoptosis
Tick cell line
id ES_45f3696f97d4d35f3d2da7e21b86ba70
oai_identifier_str oai:digital.csic.es:10261/129828
network_acronym_str ES
network_name_str España
repository_id_str
spelling Tissue-Specific Signatures in the Transcriptional Response to Anaplasma phagocytophilum Infection of Ixodes scapularis and Ixodes ricinus Tick Cell LinesAlberdi, PilarMansfield, Karen L.Manzano Román, RaúlCook, CharlotteAyllón, NievesVillar, MargaritaJohnson, NicholasFooks, Anthony R.Fuente, José de laTranscriptomicsTickRickettsiaAnaplasmaApoptosisTick cell line12 páginas, 1 tabla, 7 figuras. -- This document is protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permissionAnaplasma phagocytophilum are transmitted by Ixodes spp. ticks and have become one of the most common and relevant tick-borne pathogens due to their impact on human and animal health. Recent results have increased our understanding of the molecular interactions between Ixodes scapularis and A. phagocytophilum through the demonstration of tissue-specific molecular pathways that ensure pathogen infection, development and transmission by ticks. However, little is known about the Ixodes ricinus genes and proteins involved in the response to A. phagocytophilum infection. The tick species I. scapularis and I. ricinus are evolutionarily closely related and therefore similar responses are expected in A. phagocytophilum-infected cells. However, differences may exist between I. scapularis ISE6 and I. ricinus IRE/CTVM20 tick cells associated with tissue-specific signatures of these cell lines. To address this hypothesis, the transcriptional response to A. phagocytophilum infection was characterized by RNA sequencing and compared between I. scapularis ISE6 and I. ricinus IRE/CTVM20 tick cell lines. The transcriptional response to infection of I. scapularis ISE6 cells resembled that of tick hemocytes while the response in I. ricinus IRE/CTVM20 cells was more closely related to that reported previously in infected tick midguts. The inhibition of cell apoptosis by A. phagocytophilum appears to be a key adaptation mechanism to facilitate infection of both vertebrate and tick cells and was used to investigate further the tissue-specific response of tick cell lines to pathogen infection. The results supported a role for the intrinsic pathway in the inhibition of cell apoptosis by A. phagocytophilum infection of I. scapularis ISE6 cells. In contrast, the results in I. ricinus IRE/CTVM20 cells were similar to those obtained in tick midguts and suggested a role for the JAK/STAT pathway in the inhibition of apoptosis in tick cells infected with A. phagocytophilum. Nevertheless, tick cell lines were derived from embryonated eggs and may contain various cell populations with different morphology and behavior that could affect transcriptional response to infection. These results suggested tissue-specific signatures in I. scapularis ISE6 and I. ricinus IRE/CTVM20 tick cell line response to A. phagocytophilum infection that support their use as models for the study of tick-pathogen interactions.This research was supported by grant BFU2011-23896 from Ministerio de Economía y Competitividad (MINECO), Spain and the European Union FP7 ANTIGONE project number 278976. NA was funded by MINECO, Spain. LMH was supported by a fellowship from the University of Castilla La Mancha (UCLM), Spain. MV was supported by the Research Plan of UCLM, Spain.Peer reviewedFrontiers MediaEuropean CommissionMinisterio de Economía y Competitividad (España)Universidad de Castilla La ManchaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201620162016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/129828reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/FP7/278976http://dx.doi.org/10.3389/fcimb.2016.00020Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1298282026-05-22T06:33:51Z
dc.title.none.fl_str_mv Tissue-Specific Signatures in the Transcriptional Response to Anaplasma phagocytophilum Infection of Ixodes scapularis and Ixodes ricinus Tick Cell Lines
title Tissue-Specific Signatures in the Transcriptional Response to Anaplasma phagocytophilum Infection of Ixodes scapularis and Ixodes ricinus Tick Cell Lines
spellingShingle Tissue-Specific Signatures in the Transcriptional Response to Anaplasma phagocytophilum Infection of Ixodes scapularis and Ixodes ricinus Tick Cell Lines
Alberdi, Pilar
Transcriptomics
Tick
Rickettsia
Anaplasma
Apoptosis
Tick cell line
title_short Tissue-Specific Signatures in the Transcriptional Response to Anaplasma phagocytophilum Infection of Ixodes scapularis and Ixodes ricinus Tick Cell Lines
title_full Tissue-Specific Signatures in the Transcriptional Response to Anaplasma phagocytophilum Infection of Ixodes scapularis and Ixodes ricinus Tick Cell Lines
title_fullStr Tissue-Specific Signatures in the Transcriptional Response to Anaplasma phagocytophilum Infection of Ixodes scapularis and Ixodes ricinus Tick Cell Lines
title_full_unstemmed Tissue-Specific Signatures in the Transcriptional Response to Anaplasma phagocytophilum Infection of Ixodes scapularis and Ixodes ricinus Tick Cell Lines
title_sort Tissue-Specific Signatures in the Transcriptional Response to Anaplasma phagocytophilum Infection of Ixodes scapularis and Ixodes ricinus Tick Cell Lines
dc.creator.none.fl_str_mv Alberdi, Pilar
Mansfield, Karen L.
Manzano Román, Raúl
Cook, Charlotte
Ayllón, Nieves
Villar, Margarita
Johnson, Nicholas
Fooks, Anthony R.
Fuente, José de la
author Alberdi, Pilar
author_facet Alberdi, Pilar
Mansfield, Karen L.
Manzano Román, Raúl
Cook, Charlotte
Ayllón, Nieves
Villar, Margarita
Johnson, Nicholas
Fooks, Anthony R.
Fuente, José de la
author_role author
author2 Mansfield, Karen L.
Manzano Román, Raúl
Cook, Charlotte
Ayllón, Nieves
Villar, Margarita
Johnson, Nicholas
Fooks, Anthony R.
Fuente, José de la
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Ministerio de Economía y Competitividad (España)
Universidad de Castilla La Mancha
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Transcriptomics
Tick
Rickettsia
Anaplasma
Apoptosis
Tick cell line
topic Transcriptomics
Tick
Rickettsia
Anaplasma
Apoptosis
Tick cell line
description 12 páginas, 1 tabla, 7 figuras. -- This document is protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permission
publishDate 2016
dc.date.none.fl_str_mv 2016
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/129828
url http://hdl.handle.net/10261/129828
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/FP7/278976
http://dx.doi.org/10.3389/fcimb.2016.00020
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869407196184313857
score 15.812429

Similares en LA Referencia