Apoptosis of peritoneal leucocytes during early stages of Fasciola hepatica infections in sheep

Several immunomodulatory properties have been described in Fasciola hepatica infections. Apoptosis has been shown to be an effective mechanism to avoid the immune response in helminth infections. The aim of the present work was to study apoptosis in peritoneal leucocytes of sheep experimentally infe...

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Autores: Escamilla, A., Perez Caballero, R., Zafra, R., Bautista, María J., Pacheco Luque, Lourdes Isabel, Ruiz, M. T., Martínez Moreno, A., Martínez Cruz, María, Molina Hernández, V., Pérez, J.
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universidad Católica de Valencia San Vicente Mártir
Repositorio:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
Idioma:inglés
OAI Identifier:oai:riucv.ucv.es:20.500.12466/3853
Acceso en línea:http://hdl.handle.net/20.500.12466/3853
Access Level:acceso abierto
Palabra clave:Apoptosis
Caspase-3
Annexin-V
Peritoneal leucocytes
Fasciola hepatica
Sheep
2401.12 Parasitología Animal
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spelling Apoptosis of peritoneal leucocytes during early stages of Fasciola hepatica infections in sheepEscamilla, A.Perez Caballero, R.Zafra, R.Bautista, María J.Pacheco Luque, Lourdes IsabelRuiz, M. T.Martínez Moreno, A.Martínez Cruz, MaríaMolina Hernández, V.Pérez, J.ApoptosisCaspase-3Annexin-VPeritoneal leucocytesFasciola hepaticaSheep2401.12 Parasitología AnimalSeveral immunomodulatory properties have been described in Fasciola hepatica infections. Apoptosis has been shown to be an effective mechanism to avoid the immune response in helminth infections. The aim of the present work was to study apoptosis in peritoneal leucocytes of sheep experimentally infected with F. hepatica during the early stages of infection. Five groups (n = 5) of sheep were used. Groups 2–5 were orally infected with 200 metacercariae (mc) and sacrificed at 1, 3, 9 and 18 days post-infection (dpi), respectively. Group 1 was used as the uninfected control (UC). Apoptosis was detected using three different methods 1) immunocytochemistry (ICC) with a polyclonal antibody anti-active caspase-3; 2) an annexin V flow cytometry assay using the Annexin V-FITC/propidium iodide (PI); and 3) transmission electron microscopy (TEM). The differential leucocyte count revealed that the majority of peritoneal granulocytes were eosinophils, which increased significantly at 9 and 18 dpi with respect to the uninfected controls. The ICC study revealed that the percentage of caspase-3+ apoptotic peritoneal leucocytes increased significantly from 3 dpi onwards with respect to the uninfected controls. The flow cytometry annexin V assay detected a very significant (P < 0.001) increase of apoptotic peritoneal macrophages, lymphocytes and granulocytes, which remained higher than in the UC until 18 dpi. Transmission electron microscopy studies also confirmed the presence of apoptosis in peritoneal eosinophils at 18 dpi. This is the first report of apoptosis induced by F. hepatica in the peritoneal leucocytes of sheep in vivo. The results of this work suggest the importance of apoptosis induction for the survival of the juvenile parasites in the peritoneal migratory stages of infection.20242024-01-2520172017-04-3020172017-04-30journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12466/3853reponame:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártirinstname:Universidad Católica de Valencia San Vicente MártirInglésengEuropean Commission http://dx.doi.org/10.13039/501100000780 Horizon 2020 Framework Programme 635408 (PARAGONE)open accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:riucv.ucv.es:20.500.12466/38532026-06-19T08:32:07Z
dc.title.none.fl_str_mv Apoptosis of peritoneal leucocytes during early stages of Fasciola hepatica infections in sheep
title Apoptosis of peritoneal leucocytes during early stages of Fasciola hepatica infections in sheep
spellingShingle Apoptosis of peritoneal leucocytes during early stages of Fasciola hepatica infections in sheep
Escamilla, A.
Apoptosis
Caspase-3
Annexin-V
Peritoneal leucocytes
Fasciola hepatica
Sheep
2401.12 Parasitología Animal
title_short Apoptosis of peritoneal leucocytes during early stages of Fasciola hepatica infections in sheep
title_full Apoptosis of peritoneal leucocytes during early stages of Fasciola hepatica infections in sheep
title_fullStr Apoptosis of peritoneal leucocytes during early stages of Fasciola hepatica infections in sheep
title_full_unstemmed Apoptosis of peritoneal leucocytes during early stages of Fasciola hepatica infections in sheep
title_sort Apoptosis of peritoneal leucocytes during early stages of Fasciola hepatica infections in sheep
dc.creator.none.fl_str_mv Escamilla, A.
Perez Caballero, R.
Zafra, R.
Bautista, María J.
Pacheco Luque, Lourdes Isabel
Ruiz, M. T.
Martínez Moreno, A.
Martínez Cruz, María
Molina Hernández, V.
Pérez, J.
author Escamilla, A.
author_facet Escamilla, A.
Perez Caballero, R.
Zafra, R.
Bautista, María J.
Pacheco Luque, Lourdes Isabel
Ruiz, M. T.
Martínez Moreno, A.
Martínez Cruz, María
Molina Hernández, V.
Pérez, J.
author_role author
author2 Perez Caballero, R.
Zafra, R.
Bautista, María J.
Pacheco Luque, Lourdes Isabel
Ruiz, M. T.
Martínez Moreno, A.
Martínez Cruz, María
Molina Hernández, V.
Pérez, J.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Apoptosis
Caspase-3
Annexin-V
Peritoneal leucocytes
Fasciola hepatica
Sheep
2401.12 Parasitología Animal
topic Apoptosis
Caspase-3
Annexin-V
Peritoneal leucocytes
Fasciola hepatica
Sheep
2401.12 Parasitología Animal
description Several immunomodulatory properties have been described in Fasciola hepatica infections. Apoptosis has been shown to be an effective mechanism to avoid the immune response in helminth infections. The aim of the present work was to study apoptosis in peritoneal leucocytes of sheep experimentally infected with F. hepatica during the early stages of infection. Five groups (n = 5) of sheep were used. Groups 2–5 were orally infected with 200 metacercariae (mc) and sacrificed at 1, 3, 9 and 18 days post-infection (dpi), respectively. Group 1 was used as the uninfected control (UC). Apoptosis was detected using three different methods 1) immunocytochemistry (ICC) with a polyclonal antibody anti-active caspase-3; 2) an annexin V flow cytometry assay using the Annexin V-FITC/propidium iodide (PI); and 3) transmission electron microscopy (TEM). The differential leucocyte count revealed that the majority of peritoneal granulocytes were eosinophils, which increased significantly at 9 and 18 dpi with respect to the uninfected controls. The ICC study revealed that the percentage of caspase-3+ apoptotic peritoneal leucocytes increased significantly from 3 dpi onwards with respect to the uninfected controls. The flow cytometry annexin V assay detected a very significant (P < 0.001) increase of apoptotic peritoneal macrophages, lymphocytes and granulocytes, which remained higher than in the UC until 18 dpi. Transmission electron microscopy studies also confirmed the presence of apoptosis in peritoneal eosinophils at 18 dpi. This is the first report of apoptosis induced by F. hepatica in the peritoneal leucocytes of sheep in vivo. The results of this work suggest the importance of apoptosis induction for the survival of the juvenile parasites in the peritoneal migratory stages of infection.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-04-30
2017
2017-04-30
2024
2024-01-25
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12466/3853
url http://hdl.handle.net/20.500.12466/3853
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv European Commission http://dx.doi.org/10.13039/501100000780 Horizon 2020 Framework Programme 635408 (PARAGONE)
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
instname:Universidad Católica de Valencia San Vicente Mártir
instname_str Universidad Católica de Valencia San Vicente Mártir
reponame_str RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
collection RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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