Decrease in DHA and other fatty acids correlates with photoreceptor degeneration in retinitis pigmentosa

Fatty acids, and especially docosahexaenoic acid (DHA), are essential for photoreceptor cell integrity and are involved in the phototransduction cascade. In this study, we analyzed the changes in the fatty acid profile in the retina of the rd10 mouse, model of retinitis pigmentosa, in order to ident...

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Autores: Ruiz-Pastor, MJ, Kutsyr, O, Lax, P, Cuenca, N
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Recursos:Instituto de Investigación Biomédica y Sanitaria de Alicante (ISABIAL)
Repositorio:r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante
OAI Identifier:oai:isabial.fundanetsuite.com:p8298
Acesso em linha:https://isabial.portalinvestigacion.com/publicaciones8298
Access Level:acceso abierto
Palavra-chave:Fatty acids
DHA
Omega-3
Lipidomics
Retinitis pigmentosa
Cell death
Photoreceptors
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spelling Decrease in DHA and other fatty acids correlates with photoreceptor degeneration in retinitis pigmentosaRuiz-Pastor, MJKutsyr, OLax, PCuenca, NFatty acidsDHAOmega-3LipidomicsRetinitis pigmentosaCell deathPhotoreceptorsFatty acids, and especially docosahexaenoic acid (DHA), are essential for photoreceptor cell integrity and are involved in the phototransduction cascade. In this study, we analyzed the changes in the fatty acid profile in the retina of the rd10 mouse, model of retinitis pigmentosa, in order to identify potential risk factors for retinal degeneration and possible therapeutic approaches. Fatty acids from C57BL/6J and rd10 mouse retinas were extracted with Folch's method and analyzed by gas chromatography/mass spectrometry. Changes in retinal morphology were evaluated by immunohistochemistry. The rd10 mouse retina showed a decreased number of photoreceptor rows and alterations in photoreceptor morphology compared to C57BL/6J mice. The total amount of fatty acids dropped by 29.4% in the dystrophic retinas compared to C57BL/6J retinas. A positive correlation was found between the retinal content of specific fatty acids and the number of photoreceptor rows. We found that the amount of several short-chain and long-chain saturated fatty acids, as well as monounsaturated fatty acids, decreased in the retina of rd10 mice. Moreover, the content of the n-6 polyunsaturated fatty acid arachidonic acid and the n-3 polyunsaturated DHA decreased markedly in the dystrophic retina. The fall of DHA was more pronounced, hence the n-6/n-3 ratio was significantly increased in the diseased retina. The content of specific fatty acids in the retina decreased with photoreceptor degeneration in retinitis pigmentosa mice, with a remarkable reduction in DHA and other saturated and unsaturated fatty acids. These fatty acids could be essential for photoreceptor cell viability, and they should be evaluated for the design of therapeutical strategies and nutritional supplements.ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://isabial.portalinvestigacion.com/publicaciones8298EXPERIMENTAL EYE RESEARCHISSN: 00144835ISSNe: 10960007reponame:r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicanteinstname:Instituto de Investigación Biomédica y Sanitaria de Alicante (ISABIAL)Inglésinfo:eu-repo/semantics/openAccessoai:isabial.fundanetsuite.com:p82982026-06-12T10:20:37Z
dc.title.none.fl_str_mv Decrease in DHA and other fatty acids correlates with photoreceptor degeneration in retinitis pigmentosa
title Decrease in DHA and other fatty acids correlates with photoreceptor degeneration in retinitis pigmentosa
spellingShingle Decrease in DHA and other fatty acids correlates with photoreceptor degeneration in retinitis pigmentosa
Ruiz-Pastor, MJ
Fatty acids
DHA
Omega-3
Lipidomics
Retinitis pigmentosa
Cell death
Photoreceptors
title_short Decrease in DHA and other fatty acids correlates with photoreceptor degeneration in retinitis pigmentosa
title_full Decrease in DHA and other fatty acids correlates with photoreceptor degeneration in retinitis pigmentosa
title_fullStr Decrease in DHA and other fatty acids correlates with photoreceptor degeneration in retinitis pigmentosa
title_full_unstemmed Decrease in DHA and other fatty acids correlates with photoreceptor degeneration in retinitis pigmentosa
title_sort Decrease in DHA and other fatty acids correlates with photoreceptor degeneration in retinitis pigmentosa
dc.creator.none.fl_str_mv Ruiz-Pastor, MJ
Kutsyr, O
Lax, P
Cuenca, N
author Ruiz-Pastor, MJ
author_facet Ruiz-Pastor, MJ
Kutsyr, O
Lax, P
Cuenca, N
author_role author
author2 Kutsyr, O
Lax, P
Cuenca, N
author2_role author
author
author
dc.subject.none.fl_str_mv Fatty acids
DHA
Omega-3
Lipidomics
Retinitis pigmentosa
Cell death
Photoreceptors
topic Fatty acids
DHA
Omega-3
Lipidomics
Retinitis pigmentosa
Cell death
Photoreceptors
description Fatty acids, and especially docosahexaenoic acid (DHA), are essential for photoreceptor cell integrity and are involved in the phototransduction cascade. In this study, we analyzed the changes in the fatty acid profile in the retina of the rd10 mouse, model of retinitis pigmentosa, in order to identify potential risk factors for retinal degeneration and possible therapeutic approaches. Fatty acids from C57BL/6J and rd10 mouse retinas were extracted with Folch's method and analyzed by gas chromatography/mass spectrometry. Changes in retinal morphology were evaluated by immunohistochemistry. The rd10 mouse retina showed a decreased number of photoreceptor rows and alterations in photoreceptor morphology compared to C57BL/6J mice. The total amount of fatty acids dropped by 29.4% in the dystrophic retinas compared to C57BL/6J retinas. A positive correlation was found between the retinal content of specific fatty acids and the number of photoreceptor rows. We found that the amount of several short-chain and long-chain saturated fatty acids, as well as monounsaturated fatty acids, decreased in the retina of rd10 mice. Moreover, the content of the n-6 polyunsaturated fatty acid arachidonic acid and the n-3 polyunsaturated DHA decreased markedly in the dystrophic retina. The fall of DHA was more pronounced, hence the n-6/n-3 ratio was significantly increased in the diseased retina. The content of specific fatty acids in the retina decreased with photoreceptor degeneration in retinitis pigmentosa mice, with a remarkable reduction in DHA and other saturated and unsaturated fatty acids. These fatty acids could be essential for photoreceptor cell viability, and they should be evaluated for the design of therapeutical strategies and nutritional supplements.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://isabial.portalinvestigacion.com/publicaciones8298
url https://isabial.portalinvestigacion.com/publicaciones8298
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
publisher.none.fl_str_mv ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
dc.source.none.fl_str_mv EXPERIMENTAL EYE RESEARCH
ISSN: 00144835
ISSNe: 10960007
reponame:r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante
instname:Instituto de Investigación Biomédica y Sanitaria de Alicante (ISABIAL)
instname_str Instituto de Investigación Biomédica y Sanitaria de Alicante (ISABIAL)
reponame_str r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante
collection r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante
repository.name.fl_str_mv
repository.mail.fl_str_mv
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