FRET distance dependence from upconverting nanoparticles to quantum dots
Förster resonant energy transfer (FRET) with upconverting nanoparticles (UCNPs) as donors and quantum dots (QDs) as acceptors has been regarded as a promising tool for biosensing applications. In this work, we use time-resolved fluorescence spectroscopy to analyze the UCNP-to-QD FRET and we focus on...
| Autores: | , , , , , , , , |
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| Formato: | artículo |
| Fecha de publicación: | 2018 |
| País: | España |
| Recursos: | Universidad Complutense de Madrid (UCM) |
| Repositorio: | Docta Complutense |
| Idioma: | inglés |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/12210 |
| Acesso em linha: | https://hdl.handle.net/20.500.14352/12210 |
| Access Level: | acceso abierto |
| Palavra-chave: | 539.2:620.1 530.145 Förster resonance energy transfer Upconversion Quantum dot Óptica (Física) Partículas Teoría de los quanta 2209.19 Óptica Física 2208 Nucleónica 2210.23 Teoría Cuántica |
| Resumo: | Förster resonant energy transfer (FRET) with upconverting nanoparticles (UCNPs) as donors and quantum dots (QDs) as acceptors has been regarded as a promising tool for biosensing applications. In this work, we use time-resolved fluorescence spectroscopy to analyze the UCNP-to-QD FRET and we focus on the most relevant parameter of the FRET phenomenon, UCNP-QD distance. This distance is controlled by a nanometric silica shell around the UCNP surface. We theoretically reproduce the experimental results applying FRET theory to the distribution of emitting erbium ions in the UCNP. This simple model allows us to estimate the contribution of every erbium ion to the final FRET response and to explore different strategies to improve FRET efficiency. |
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