A short RNA stem–loop is necessary and sufficient for repression of gene expression during early logarithmic phase in trypanosomes

We have compared the transcriptomes of cultured procyclic Trypanosoma brucei cells in early and late logarithmic phases and found that ∼200 mRNAs were differentially regulated. In late log phase cells, the most upregulated mRNA encoded the nucleobase transporter NT8. The 3′ untranslated region (UTR)...

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Detalles Bibliográficos
Autores: Fernández-Moya, Sandra M., Carrington, Mark, Estévez, Antonio M.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/137113
Acceso en línea:http://hdl.handle.net/10261/137113
Access Level:acceso abierto
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spelling A short RNA stem–loop is necessary and sufficient for repression of gene expression during early logarithmic phase in trypanosomesFernández-Moya, Sandra M.Carrington, MarkEstévez, Antonio M.We have compared the transcriptomes of cultured procyclic Trypanosoma brucei cells in early and late logarithmic phases and found that ∼200 mRNAs were differentially regulated. In late log phase cells, the most upregulated mRNA encoded the nucleobase transporter NT8. The 3′ untranslated region (UTR) of NT8 contains a short stem–loop cis-element that is necessary for the regulation of NT8 expression in response to external purine levels. When placed in the 3′-UTR of an unregulated transcript, the cis-element is sufficient to confer regulation in response to purines. To our knowledge, this is the first example of a discrete RNA element that can autonomously regulate gene expression in trypanosomes in response to an external factor and reveals an unprecedented purine-dependent signaling pathway that controls gene expression in eukaryotes.Ministerio de Ciencia e Innovación [BFU 2009-07510 to A.M.E.]; The Wellcome Trust [085956 to M.C.]; The Royal Society Joint Project [2008/R2 to M.C. and A.M.E.] Funding for open access charge: The Wellcome Trust.Peer reviewedOxford University PressConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201620162014info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/137113reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://nar.oxfordjournals.org/content/early/2014/05/09/nar.gku358.fullSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1371132026-05-22T06:33:51Z
dc.title.none.fl_str_mv A short RNA stem–loop is necessary and sufficient for repression of gene expression during early logarithmic phase in trypanosomes
title A short RNA stem–loop is necessary and sufficient for repression of gene expression during early logarithmic phase in trypanosomes
spellingShingle A short RNA stem–loop is necessary and sufficient for repression of gene expression during early logarithmic phase in trypanosomes
Fernández-Moya, Sandra M.
title_short A short RNA stem–loop is necessary and sufficient for repression of gene expression during early logarithmic phase in trypanosomes
title_full A short RNA stem–loop is necessary and sufficient for repression of gene expression during early logarithmic phase in trypanosomes
title_fullStr A short RNA stem–loop is necessary and sufficient for repression of gene expression during early logarithmic phase in trypanosomes
title_full_unstemmed A short RNA stem–loop is necessary and sufficient for repression of gene expression during early logarithmic phase in trypanosomes
title_sort A short RNA stem–loop is necessary and sufficient for repression of gene expression during early logarithmic phase in trypanosomes
dc.creator.none.fl_str_mv Fernández-Moya, Sandra M.
Carrington, Mark
Estévez, Antonio M.
author Fernández-Moya, Sandra M.
author_facet Fernández-Moya, Sandra M.
Carrington, Mark
Estévez, Antonio M.
author_role author
author2 Carrington, Mark
Estévez, Antonio M.
author2_role author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description We have compared the transcriptomes of cultured procyclic Trypanosoma brucei cells in early and late logarithmic phases and found that ∼200 mRNAs were differentially regulated. In late log phase cells, the most upregulated mRNA encoded the nucleobase transporter NT8. The 3′ untranslated region (UTR) of NT8 contains a short stem–loop cis-element that is necessary for the regulation of NT8 expression in response to external purine levels. When placed in the 3′-UTR of an unregulated transcript, the cis-element is sufficient to confer regulation in response to purines. To our knowledge, this is the first example of a discrete RNA element that can autonomously regulate gene expression in trypanosomes in response to an external factor and reveals an unprecedented purine-dependent signaling pathway that controls gene expression in eukaryotes.
publishDate 2014
dc.date.none.fl_str_mv 2014
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/137113
url http://hdl.handle.net/10261/137113
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://nar.oxfordjournals.org/content/early/2014/05/09/nar.gku358.full
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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