Genetic barcoding of Ecuadorian epilithic diatom species suitable as water quality bioindicators

Diatom identification is a key step in using these microorganisms as water quality bioindicators. Morphological diagnosis is a difficult task due to the enormous number of species and their microscopic size. This can be overcome using molecular tools to complement the diagnosis. The main goal of thi...

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Detalles Bibliográficos
Autores: Ballesteros Redondo, María Isabel, Castillejo, Pablo, Haro, Adriana Paulina, Montes, Cuthy Cristina, Heinrich, Carla, Lobo, Eduardo Alexis
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/102494
Acceso en línea:https://hdl.handle.net/20.500.14352/102494
Access Level:acceso abierto
Palabra clave:579.25
Microbiología (Biología)
Genética
2414 Microbiología
2409 Genética
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spelling Genetic barcoding of Ecuadorian epilithic diatom species suitable as water quality bioindicatorsBallesteros Redondo, María IsabelCastillejo, PabloHaro, Adriana PaulinaMontes, Cuthy CristinaHeinrich, CarlaLobo, Eduardo Alexis579.25Microbiología (Biología)Genética2414 Microbiología2409 GenéticaDiatom identification is a key step in using these microorganisms as water quality bioindicators. Morphological diagnosis is a difficult task due to the enormous number of species and their microscopic size. This can be overcome using molecular tools to complement the diagnosis. The main goal of this work was to obtain the DNA barcode of Ecuadorian epilithic diatoms with a wide geographical distribution, a well-defined ecological range and characteristics that allow them to be reliable indicator species. Unialgal diatom cultures were obtained from environmental samples of Ecuadorian Andean streams. Morphological characterization of cultures was carried out under SEM microscopy. For molecular characterization, 18SV4 and rbcL barcodes were sequenced from each strain and blasted against a GenBank database. A phylogenetic tree for each barcode was constructed using the ML method including sequences of strains of the studied species from different geographical locations. The results showed the following five species to be suitable as bioindicators and these were isolated. Sellaphora seminulum (strain JA01b, c), Nitzschia fonticola (strain SP02a) and N. palea (strain CA01a) are tolerant to eutrophication; Eolimna minima (strain CH02a) is a mesotrophic water bioindicator, and Achnanthidium minutissimum (strain JA01a) is an oligotrophic water bioindicator. The comparison with the GenBank database of the barcoding regions supported the morphological identification. The barcoding sequences of the strains showed a high percentage of identity with the sequences reported in INSDC databases for the same species. The topology of the phylogenetic trees demonstrates that epilithic diatoms from Ecuador are closely related to those of same species isolated from other geographical regions. This study is a first attempt to establish a morphological and molecular taxonomic reference library for neotropical diatoms. This study demonstrates that it would be feasible to use the existing barcoding data for diatoms to develop molecular tools for the bioassessment of aquatic ecosystems in the Ecuadorian Andean region.Académie des SciencesUniversidad Complutense de Madrid20242024-04-0220202020-01-0120202020-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/102494reponame:Docta Complutenseinstname:Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1024942025-08-02T12:44:52Z
dc.title.none.fl_str_mv Genetic barcoding of Ecuadorian epilithic diatom species suitable as water quality bioindicators
title Genetic barcoding of Ecuadorian epilithic diatom species suitable as water quality bioindicators
spellingShingle Genetic barcoding of Ecuadorian epilithic diatom species suitable as water quality bioindicators
Ballesteros Redondo, María Isabel
579.25
Microbiología (Biología)
Genética
2414 Microbiología
2409 Genética
title_short Genetic barcoding of Ecuadorian epilithic diatom species suitable as water quality bioindicators
title_full Genetic barcoding of Ecuadorian epilithic diatom species suitable as water quality bioindicators
title_fullStr Genetic barcoding of Ecuadorian epilithic diatom species suitable as water quality bioindicators
title_full_unstemmed Genetic barcoding of Ecuadorian epilithic diatom species suitable as water quality bioindicators
title_sort Genetic barcoding of Ecuadorian epilithic diatom species suitable as water quality bioindicators
dc.creator.none.fl_str_mv Ballesteros Redondo, María Isabel
Castillejo, Pablo
Haro, Adriana Paulina
Montes, Cuthy Cristina
Heinrich, Carla
Lobo, Eduardo Alexis
author Ballesteros Redondo, María Isabel
author_facet Ballesteros Redondo, María Isabel
Castillejo, Pablo
Haro, Adriana Paulina
Montes, Cuthy Cristina
Heinrich, Carla
Lobo, Eduardo Alexis
author_role author
author2 Castillejo, Pablo
Haro, Adriana Paulina
Montes, Cuthy Cristina
Heinrich, Carla
Lobo, Eduardo Alexis
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 579.25
Microbiología (Biología)
Genética
2414 Microbiología
2409 Genética
topic 579.25
Microbiología (Biología)
Genética
2414 Microbiología
2409 Genética
description Diatom identification is a key step in using these microorganisms as water quality bioindicators. Morphological diagnosis is a difficult task due to the enormous number of species and their microscopic size. This can be overcome using molecular tools to complement the diagnosis. The main goal of this work was to obtain the DNA barcode of Ecuadorian epilithic diatoms with a wide geographical distribution, a well-defined ecological range and characteristics that allow them to be reliable indicator species. Unialgal diatom cultures were obtained from environmental samples of Ecuadorian Andean streams. Morphological characterization of cultures was carried out under SEM microscopy. For molecular characterization, 18SV4 and rbcL barcodes were sequenced from each strain and blasted against a GenBank database. A phylogenetic tree for each barcode was constructed using the ML method including sequences of strains of the studied species from different geographical locations. The results showed the following five species to be suitable as bioindicators and these were isolated. Sellaphora seminulum (strain JA01b, c), Nitzschia fonticola (strain SP02a) and N. palea (strain CA01a) are tolerant to eutrophication; Eolimna minima (strain CH02a) is a mesotrophic water bioindicator, and Achnanthidium minutissimum (strain JA01a) is an oligotrophic water bioindicator. The comparison with the GenBank database of the barcoding regions supported the morphological identification. The barcoding sequences of the strains showed a high percentage of identity with the sequences reported in INSDC databases for the same species. The topology of the phylogenetic trees demonstrates that epilithic diatoms from Ecuador are closely related to those of same species isolated from other geographical regions. This study is a first attempt to establish a morphological and molecular taxonomic reference library for neotropical diatoms. This study demonstrates that it would be feasible to use the existing barcoding data for diatoms to develop molecular tools for the bioassessment of aquatic ecosystems in the Ecuadorian Andean region.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01
2020
2020-01-01
2024
2024-04-02
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/102494
url https://hdl.handle.net/20.500.14352/102494
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Académie des Sciences
publisher.none.fl_str_mv Académie des Sciences
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:
instname_str
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,647109

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