New dockside eDNA based protocol to detect the seaweed Asparagopsis armata evaluated by stakeholders

Early detection of invasive species is crucial to deal effectively with biological invasions in ports, which are hotspots of species introductions. In this study, a simplified end-time PCR methodology conducted on eDNA from water samples was developed for rapid detection of the invasive seaweed Aspa...

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Detalles Bibliográficos
Autores: Ibabe, Aitor, Menéndez-Telena, Deva, Soto López, Verónica, Ardura, Alba, Arias, Andrés, Bartolomé, Marlene, Borrell, Yaisel J., Fernández, Sara, Machado-Schiaffino Ferrer, Gonzalo|||0000-0002-4049-3247, Mateo, Juan L., Dopico, Eduardo, García-Vázquez, Eva
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universidad de Oviedo (UNIOVI)
Repositorio:RUO. Repositorio Institucional de la Universidad de Oviedo
Idioma:inglés
OAI Identifier:oai:digibuo.uniovi.es:10651/79215
Acceso en línea:https://hdl.handle.net/10651/79215
https://dx.doi.org/10.1016/j.marpolbul.2024.116259
Access Level:acceso abierto
Palabra clave:Asparagopsis armata
Early detection
eDNA
Invasive species
Ports Stakeholders
Descripción
Sumario:Early detection of invasive species is crucial to deal effectively with biological invasions in ports, which are hotspots of species introductions. In this study, a simplified end-time PCR methodology conducted on eDNA from water samples was developed for rapid detection of the invasive seaweed Asparagopsis armata (four hours from water collection to result visualization). It was tested dockside in four international Spanish ports in presence of stakeholders, whose feedback was obtained to explore the real applicability of this biotechnology. Although biological invasions were not a main concern for them, results indicate a unanimous approval of the methodology by the stakeholders, having detected the presence of A. armata in three of the ports. Stakeholders suggested further developments for easier application of the tool and multiple species detection, to be adopted for the control of invasive species in ports.