Assessment of Tufted Puffin Fratercula cirrhata diet using DNA metabarcoding

Investigating trophic relationships can be critical for understanding relationships between marine predators and their prey. DNA analysis of feces is used increasingly as a non-invasive method to uncover seabird dietary patterns across space and time. Tufted Puffins Fratercula cirrhata are listed as...

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Autores: Good, Thomas P., Shelton, Andrew O., Wells, Abigail H., Ramón-Laca, Ana, Gallego, Ramon, Hodum, Peter J., Pearson, Scott F.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/388340
Acceso en línea:http://hdl.handle.net/10261/388340
Access Level:acceso abierto
Palabra clave:Metabarcoding
Fecal DNA
Tufted Puffin
California Current
http://metadata.un.org/sdg/14
Conserve and sustainably use the oceans, seas and marine resources for sustainable development
Protect, restore and promote sustainable use of terrestrial ecosystems, sustainably manage forests, combat desertification, and halt and reverse land degradation and halt biodiversity loss
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spelling Assessment of Tufted Puffin Fratercula cirrhata diet using DNA metabarcodingGood, Thomas P.Shelton, Andrew O.Wells, Abigail H.Ramón-Laca, AnaGallego, RamonHodum, Peter J.Pearson, Scott F.MetabarcodingFecal DNATufted PuffinCalifornia Currenthttp://metadata.un.org/sdg/14Conserve and sustainably use the oceans, seas and marine resources for sustainable developmentProtect, restore and promote sustainable use of terrestrial ecosystems, sustainably manage forests, combat desertification, and halt and reverse land degradation and halt biodiversity lossInvestigating trophic relationships can be critical for understanding relationships between marine predators and their prey. DNA analysis of feces is used increasingly as a non-invasive method to uncover seabird dietary patterns across space and time. Tufted Puffins Fratercula cirrhata are listed as Endangered in the state of Washington (WA), USA, and reduced prey availability is thought to be a key factor in the species’ decline. Recent information on Tufted Puffin diet is lacking, and present opportunities for direct diet observation are limited. We conducted a pilot study to characterize Tufted Puffin diet on Destruction Island, WA, in 2019 using DNA metabarcoding of feces from burrow entrances and from soil in nesting chambers. Smelt (Osmeridae) and rockfish (Scorpaenidae) were detected in all fecal samples, along with a variety of other fish taxa, squid, crab, and shrimp. Smelt was detected in most soil samples, as were a variety of other fish, crustaceans, and terrestrial insects. While DNA metabarcoding detected several taxa also identified in Tufted Puffin bill-loads in 2019, fecal and soil samples detected multiple taxa not identified in bill-loads. It appears that Tufted Puffin diet can be characterized using DNA metabarcoding, provided that fecal samples are of sufficient quality and that contamination is minimized. Amplifying prey DNA from soil samples opens opportunities for sampling burrows after breeding, which would minimize disruptions to study colonies. Future strategies to characterize Tufted Puffin diet could combine direct observation and DNA metabarcoding methods where possible and could focus on the latter methods where observation is difficult. These non-destructive and non-disruptive methods hold promise for characterizing the diet of other burrow-nesting species of conservation concern.For their support on this project, the authors thank Linda Park and Krista Nichols, the former and present managers of the Northwest Fisheries Science Center’s (NWFSC) Genetics and Evolution Program. This project received funding from the NWFSC Internal Grant Program, along with in-kind support from the Washington Department of Fish and Wildlife and NOAA National Marine Fisheries Service through the California Current Integrated Ecosystem Assessment program.Peer reviewedPacific Seabird GroupNorthwest Fisheries Science Center (US)Washington Department of Fish & WildlifeNational Oceanic and Atmospheric Administration (US)Ramón-Laca, Ana [0000-0002-9204-6932]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/388340reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.5038/2074-1235.52.2.1587Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3883402026-05-22T06:33:51Z
dc.title.none.fl_str_mv Assessment of Tufted Puffin Fratercula cirrhata diet using DNA metabarcoding
title Assessment of Tufted Puffin Fratercula cirrhata diet using DNA metabarcoding
spellingShingle Assessment of Tufted Puffin Fratercula cirrhata diet using DNA metabarcoding
Good, Thomas P.
Metabarcoding
Fecal DNA
Tufted Puffin
California Current
http://metadata.un.org/sdg/14
Conserve and sustainably use the oceans, seas and marine resources for sustainable development
Protect, restore and promote sustainable use of terrestrial ecosystems, sustainably manage forests, combat desertification, and halt and reverse land degradation and halt biodiversity loss
title_short Assessment of Tufted Puffin Fratercula cirrhata diet using DNA metabarcoding
title_full Assessment of Tufted Puffin Fratercula cirrhata diet using DNA metabarcoding
title_fullStr Assessment of Tufted Puffin Fratercula cirrhata diet using DNA metabarcoding
title_full_unstemmed Assessment of Tufted Puffin Fratercula cirrhata diet using DNA metabarcoding
title_sort Assessment of Tufted Puffin Fratercula cirrhata diet using DNA metabarcoding
dc.creator.none.fl_str_mv Good, Thomas P.
Shelton, Andrew O.
Wells, Abigail H.
Ramón-Laca, Ana
Gallego, Ramon
Hodum, Peter J.
Pearson, Scott F.
author Good, Thomas P.
author_facet Good, Thomas P.
Shelton, Andrew O.
Wells, Abigail H.
Ramón-Laca, Ana
Gallego, Ramon
Hodum, Peter J.
Pearson, Scott F.
author_role author
author2 Shelton, Andrew O.
Wells, Abigail H.
Ramón-Laca, Ana
Gallego, Ramon
Hodum, Peter J.
Pearson, Scott F.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Northwest Fisheries Science Center (US)
Washington Department of Fish & Wildlife
National Oceanic and Atmospheric Administration (US)
Ramón-Laca, Ana [0000-0002-9204-6932]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Metabarcoding
Fecal DNA
Tufted Puffin
California Current
http://metadata.un.org/sdg/14
Conserve and sustainably use the oceans, seas and marine resources for sustainable development
Protect, restore and promote sustainable use of terrestrial ecosystems, sustainably manage forests, combat desertification, and halt and reverse land degradation and halt biodiversity loss
topic Metabarcoding
Fecal DNA
Tufted Puffin
California Current
http://metadata.un.org/sdg/14
Conserve and sustainably use the oceans, seas and marine resources for sustainable development
Protect, restore and promote sustainable use of terrestrial ecosystems, sustainably manage forests, combat desertification, and halt and reverse land degradation and halt biodiversity loss
description Investigating trophic relationships can be critical for understanding relationships between marine predators and their prey. DNA analysis of feces is used increasingly as a non-invasive method to uncover seabird dietary patterns across space and time. Tufted Puffins Fratercula cirrhata are listed as Endangered in the state of Washington (WA), USA, and reduced prey availability is thought to be a key factor in the species’ decline. Recent information on Tufted Puffin diet is lacking, and present opportunities for direct diet observation are limited. We conducted a pilot study to characterize Tufted Puffin diet on Destruction Island, WA, in 2019 using DNA metabarcoding of feces from burrow entrances and from soil in nesting chambers. Smelt (Osmeridae) and rockfish (Scorpaenidae) were detected in all fecal samples, along with a variety of other fish taxa, squid, crab, and shrimp. Smelt was detected in most soil samples, as were a variety of other fish, crustaceans, and terrestrial insects. While DNA metabarcoding detected several taxa also identified in Tufted Puffin bill-loads in 2019, fecal and soil samples detected multiple taxa not identified in bill-loads. It appears that Tufted Puffin diet can be characterized using DNA metabarcoding, provided that fecal samples are of sufficient quality and that contamination is minimized. Amplifying prey DNA from soil samples opens opportunities for sampling burrows after breeding, which would minimize disruptions to study colonies. Future strategies to characterize Tufted Puffin diet could combine direct observation and DNA metabarcoding methods where possible and could focus on the latter methods where observation is difficult. These non-destructive and non-disruptive methods hold promise for characterizing the diet of other burrow-nesting species of conservation concern.
publishDate 2024
dc.date.none.fl_str_mv 2024
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/388340
url http://hdl.handle.net/10261/388340
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.5038/2074-1235.52.2.1587
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Pacific Seabird Group
publisher.none.fl_str_mv Pacific Seabird Group
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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