A simple method to measure renal function in swine by the plasma clearance of iohexol

There is no simple method to measure glomerular filtration rate (GFR) in swine, an established model for studying renal disease. We developed a protocol to measure GFR in conscious swine by using the plasma clearance of iohexol. We used two groups, test and validation, with eight animals each. Ten m...

Descripción completa

Detalles Bibliográficos
Autores: Luis-Lima, Sergio, Garcia-Contreras, Consolacion, Vázquez-Gómez, M., Astiz, Susana, Carrara, F., Gaspari, Flavio, Negrín-Mena, N., Jiménez-Sosa, Alejandro, Jiménez-Hernández, Hugo, González De Bulnes, Antonio, Porrini, Esteban
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/290876
Acceso en línea:http://hdl.handle.net/10261/290876
Access Level:acceso abierto
Palabra clave:Renal function
Iohexol plasma clearance
Swine model
id ES_44f042ef7ae2cf7c98b52f1e4479cdb4
oai_identifier_str oai:digital.csic.es:10261/290876
network_acronym_str ES
network_name_str España
repository_id_str
spelling A simple method to measure renal function in swine by the plasma clearance of iohexolLuis-Lima, SergioGarcia-Contreras, ConsolacionVázquez-Gómez, M.Astiz, SusanaCarrara, F.Gaspari, FlavioNegrín-Mena, N.Jiménez-Sosa, AlejandroJiménez-Hernández, HugoGonzález De Bulnes, AntonioPorrini, EstebanRenal functionIohexol plasma clearanceSwine modelThere is no simple method to measure glomerular filtration rate (GFR) in swine, an established model for studying renal disease. We developed a protocol to measure GFR in conscious swine by using the plasma clearance of iohexol. We used two groups, test and validation, with eight animals each. Ten milliliters of iohexol (6.47 g) was injected into the marginal auricular vein and blood samples (3 mL) were collected from the orbital sinus at different points after injection. GFR was determined using two models: two-compartment (CL2: all samples) and one-compartment (CL1: the last six samples). In the test group, CL1 overestimated CL2 by ~30%: CL2 = 245 ± 93 and CL1 = 308 ± 123 mL/min. This error was corrected by a first-order polynomial quadratic equation to CL1, which was considered the simplified method: SM = −47.909 + (1.176xCL1) − (0.00063968xCL12). The SM showed narrow limits of agreement with CL2, a concordance correlation of 0.97, and a total deviation index of 14.73%. Similar results were obtained for the validation group. This protocol is reliable, reproducible, can be performed in conscious animals, uses a single dose of the marker, and requires a reduced number of samples, and avoids urine collection. Finally, it presents a significant improvement in animal welfare conditions and handling necessities in experimental trials.Peer reviewedMultidisciplinary Digital Publishing InstituteLuis-Lima, Sergio [0000-0003-3540-8252]Gaspari, Flavio [0000-0001-5941-3481]Porrini, Esteban [0000-0002-3056-4662]García-Contreras, Consolación [0000-0003-4624-2585]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/290876reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2908762026-05-22T06:33:51Z
dc.title.none.fl_str_mv A simple method to measure renal function in swine by the plasma clearance of iohexol
title A simple method to measure renal function in swine by the plasma clearance of iohexol
spellingShingle A simple method to measure renal function in swine by the plasma clearance of iohexol
Luis-Lima, Sergio
Renal function
Iohexol plasma clearance
Swine model
title_short A simple method to measure renal function in swine by the plasma clearance of iohexol
title_full A simple method to measure renal function in swine by the plasma clearance of iohexol
title_fullStr A simple method to measure renal function in swine by the plasma clearance of iohexol
title_full_unstemmed A simple method to measure renal function in swine by the plasma clearance of iohexol
title_sort A simple method to measure renal function in swine by the plasma clearance of iohexol
dc.creator.none.fl_str_mv Luis-Lima, Sergio
Garcia-Contreras, Consolacion
Vázquez-Gómez, M.
Astiz, Susana
Carrara, F.
Gaspari, Flavio
Negrín-Mena, N.
Jiménez-Sosa, Alejandro
Jiménez-Hernández, Hugo
González De Bulnes, Antonio
Porrini, Esteban
author Luis-Lima, Sergio
author_facet Luis-Lima, Sergio
Garcia-Contreras, Consolacion
Vázquez-Gómez, M.
Astiz, Susana
Carrara, F.
Gaspari, Flavio
Negrín-Mena, N.
Jiménez-Sosa, Alejandro
Jiménez-Hernández, Hugo
González De Bulnes, Antonio
Porrini, Esteban
author_role author
author2 Garcia-Contreras, Consolacion
Vázquez-Gómez, M.
Astiz, Susana
Carrara, F.
Gaspari, Flavio
Negrín-Mena, N.
Jiménez-Sosa, Alejandro
Jiménez-Hernández, Hugo
González De Bulnes, Antonio
Porrini, Esteban
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Luis-Lima, Sergio [0000-0003-3540-8252]
Gaspari, Flavio [0000-0001-5941-3481]
Porrini, Esteban [0000-0002-3056-4662]
García-Contreras, Consolación [0000-0003-4624-2585]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Renal function
Iohexol plasma clearance
Swine model
topic Renal function
Iohexol plasma clearance
Swine model
description There is no simple method to measure glomerular filtration rate (GFR) in swine, an established model for studying renal disease. We developed a protocol to measure GFR in conscious swine by using the plasma clearance of iohexol. We used two groups, test and validation, with eight animals each. Ten milliliters of iohexol (6.47 g) was injected into the marginal auricular vein and blood samples (3 mL) were collected from the orbital sinus at different points after injection. GFR was determined using two models: two-compartment (CL2: all samples) and one-compartment (CL1: the last six samples). In the test group, CL1 overestimated CL2 by ~30%: CL2 = 245 ± 93 and CL1 = 308 ± 123 mL/min. This error was corrected by a first-order polynomial quadratic equation to CL1, which was considered the simplified method: SM = −47.909 + (1.176xCL1) − (0.00063968xCL12). The SM showed narrow limits of agreement with CL2, a concordance correlation of 0.97, and a total deviation index of 14.73%. Similar results were obtained for the validation group. This protocol is reliable, reproducible, can be performed in conscious animals, uses a single dose of the marker, and requires a reduced number of samples, and avoids urine collection. Finally, it presents a significant improvement in animal welfare conditions and handling necessities in experimental trials.
publishDate 2018
dc.date.none.fl_str_mv 2018
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/290876
url http://hdl.handle.net/10261/290876
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869407128123342848
score 15.811543

Similares en LA Referencia