Bacterial division ring stabilizing ZapA versus destabilizing SlmA modulate FtsZ switching between biomolecular condensates and polymers

16 p.-7 fig.

Detalhes bibliográficos
Autores: Monterroso, Begoña, Robles-Ramos, Miguel A., Sobrinos-Sanguino, Marta, Luque-Ortega, Juan Román, Alfonso, Carlos, Margolin, William, Rivas, Germán, Zorrilla, Silvia
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/296728
Acesso em linha:http://hdl.handle.net/10261/296728
Access Level:acceso abierto
Palavra-chave:Biomolecular condensates
Bacterial division
Crowding-driven phase separation
Membraneless compartments
Subcellular organization
Biochemical reconstitution in cytomimetic media
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spelling Bacterial division ring stabilizing ZapA versus destabilizing SlmA modulate FtsZ switching between biomolecular condensates and polymersMonterroso, BegoñaRobles-Ramos, Miguel A.Sobrinos-Sanguino, MartaLuque-Ortega, Juan RománAlfonso, CarlosMargolin, WilliamRivas, GermánZorrilla, SilviaBiomolecular condensatesBacterial divisionCrowding-driven phase separationMembraneless compartmentsSubcellular organizationBiochemical reconstitution in cytomimetic media16 p.-7 fig.Cytokinesis is a fundamental process for bacterial survival and proliferation, involving the formation of a ring by filaments of the GTPase FtsZ, spatio-temporally regulated through the coordinated action of several factors. The mechanisms of this regulation remain largely unsolved, but the inhibition of FtsZ polymerization by the nucleoid occlusion factor SlmA and filament stabilization by the widely conserved cross-linking protein ZapA are known to play key roles. It was recently described that FtsZ, SlmA and its target DNA sequences (SlmA-binding sequence (SBS)) form phase-separated biomolecular condensates, a type of structure associated with cellular compartmentalization and resistance to stress. Using biochemical reconstitution and orthogonal biophysical approaches, we show that FtsZ-SlmA-SBS condensates captured ZapA in crowding conditions and when encapsulated inside cell-like microfluidics microdroplets. We found that, through non-competitive binding, the nucleotide-dependent FtsZ condensate/polymer interconversion was regulated by the ZapA/SlmA ratio. This suggests a highly concentration-responsive tuning of the interconversion that favours FtsZ polymer stabilization by ZapA under conditions mimicking intracellular crowding. These results highlight the importance of biomolecular condensates as concentration hubs for bacterial division factors, which can provide clues to their role in cell function and bacterial survival of stress conditions, such as those generated by antibiotic treatment.This work was supported by the Spanish Ministerio de Ciencia e Innovación (grant nos. PID2019-104544GB-I00/AEI/10.13039/501100011033, to G.R. and S.Z.) and by the National Institutes of Health (grant no. GM131705, to W.M.). M.Á.R.R. and M.S.S. were supported by the Agencia Estatal de Investigación and the European Social Fund through grants BES-2017-082003 and PTA2020-018219-I/AEI/10.13039/501100011033), respectively. The Systems Biochemistry of Bacterial Division group (CIB Margarita Salas) participates in the CSIC Conexiones LifeHUB (grant no. PIE-202120E047). The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.Peer reviewedRoyal Society (Great Britain)Ministerio de Ciencia e Innovación (España)National Institutes of Health (US)Agencia Estatal de Investigación (España)Consejo Superior de Investigaciones Científicas (España)Monterroso, Begoña [0000-0003-2538-084X]Robles-Ramos, Miguel A. [0000-0002-8522-1202]Sobrinos-Sanguino, Marta [0000-0002-3479-9100]Luque-Ortega, Juan Román [0000-0003-3206-7480]Alfonso, Carlos [0000-0001-7165-4800]Margolin, William [0000-0001-6557-7706]Rivas, Germán [0000-0003-3450-7478]Zorrilla, Silvia [0000-0002-6309-9058]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/296728reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104544GB-I00https://doi.org/10.1098/rsob.220324Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2967282026-05-22T06:33:51Z
dc.title.none.fl_str_mv Bacterial division ring stabilizing ZapA versus destabilizing SlmA modulate FtsZ switching between biomolecular condensates and polymers
title Bacterial division ring stabilizing ZapA versus destabilizing SlmA modulate FtsZ switching between biomolecular condensates and polymers
spellingShingle Bacterial division ring stabilizing ZapA versus destabilizing SlmA modulate FtsZ switching between biomolecular condensates and polymers
Monterroso, Begoña
Biomolecular condensates
Bacterial division
Crowding-driven phase separation
Membraneless compartments
Subcellular organization
Biochemical reconstitution in cytomimetic media
title_short Bacterial division ring stabilizing ZapA versus destabilizing SlmA modulate FtsZ switching between biomolecular condensates and polymers
title_full Bacterial division ring stabilizing ZapA versus destabilizing SlmA modulate FtsZ switching between biomolecular condensates and polymers
title_fullStr Bacterial division ring stabilizing ZapA versus destabilizing SlmA modulate FtsZ switching between biomolecular condensates and polymers
title_full_unstemmed Bacterial division ring stabilizing ZapA versus destabilizing SlmA modulate FtsZ switching between biomolecular condensates and polymers
title_sort Bacterial division ring stabilizing ZapA versus destabilizing SlmA modulate FtsZ switching between biomolecular condensates and polymers
dc.creator.none.fl_str_mv Monterroso, Begoña
Robles-Ramos, Miguel A.
Sobrinos-Sanguino, Marta
Luque-Ortega, Juan Román
Alfonso, Carlos
Margolin, William
Rivas, Germán
Zorrilla, Silvia
author Monterroso, Begoña
author_facet Monterroso, Begoña
Robles-Ramos, Miguel A.
Sobrinos-Sanguino, Marta
Luque-Ortega, Juan Román
Alfonso, Carlos
Margolin, William
Rivas, Germán
Zorrilla, Silvia
author_role author
author2 Robles-Ramos, Miguel A.
Sobrinos-Sanguino, Marta
Luque-Ortega, Juan Román
Alfonso, Carlos
Margolin, William
Rivas, Germán
Zorrilla, Silvia
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
National Institutes of Health (US)
Agencia Estatal de Investigación (España)
Consejo Superior de Investigaciones Científicas (España)
Monterroso, Begoña [0000-0003-2538-084X]
Robles-Ramos, Miguel A. [0000-0002-8522-1202]
Sobrinos-Sanguino, Marta [0000-0002-3479-9100]
Luque-Ortega, Juan Román [0000-0003-3206-7480]
Alfonso, Carlos [0000-0001-7165-4800]
Margolin, William [0000-0001-6557-7706]
Rivas, Germán [0000-0003-3450-7478]
Zorrilla, Silvia [0000-0002-6309-9058]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Biomolecular condensates
Bacterial division
Crowding-driven phase separation
Membraneless compartments
Subcellular organization
Biochemical reconstitution in cytomimetic media
topic Biomolecular condensates
Bacterial division
Crowding-driven phase separation
Membraneless compartments
Subcellular organization
Biochemical reconstitution in cytomimetic media
description 16 p.-7 fig.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/296728
url http://hdl.handle.net/10261/296728
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104544GB-I00
https://doi.org/10.1098/rsob.220324
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Royal Society (Great Britain)
publisher.none.fl_str_mv Royal Society (Great Britain)
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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