Quantitative Mid-Infrared Plasmonic Biosensing on Scalable Graphene Nanostructures

Graphene nanostructures, exhibiting tunable and nanoscale-confined mid-infrared (mid-IR) plasmons, prevail as a powerful spectroscopic platform for novel surface-enhanced molecular identification. Particularly, graphene shows exciting opportunities for biosensing applications due to its versatile fu...

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Autores: Bareza, Nestor Jr., Wajs, Ewelina, Paulillo, Bruno, Tullila, Antti, Jaatinen, Hannakaisa, Milani, Roberto, Dore, Camilla, Mihi, Agustín, Nevanen, Tarja K., Pruneri, Valerio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/341289
Acceso en línea:http://hdl.handle.net/10261/341289
https://api.elsevier.com/content/abstract/scopus_id/85142776536
Access Level:acceso abierto
Palabra clave:Antibody
Graphene plasmonics
Mid-infrared biosensor
SEIRA
Vitamin B 12
id ES_44e2ee7d21a095ebdbb4d8a273b39bab
oai_identifier_str oai:digital.csic.es:10261/341289
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network_name_str España
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dc.title.none.fl_str_mv Quantitative Mid-Infrared Plasmonic Biosensing on Scalable Graphene Nanostructures
title Quantitative Mid-Infrared Plasmonic Biosensing on Scalable Graphene Nanostructures
spellingShingle Quantitative Mid-Infrared Plasmonic Biosensing on Scalable Graphene Nanostructures
Bareza, Nestor Jr.
Antibody
Graphene plasmonics
Mid-infrared biosensor
SEIRA
Vitamin B 12
title_short Quantitative Mid-Infrared Plasmonic Biosensing on Scalable Graphene Nanostructures
title_full Quantitative Mid-Infrared Plasmonic Biosensing on Scalable Graphene Nanostructures
title_fullStr Quantitative Mid-Infrared Plasmonic Biosensing on Scalable Graphene Nanostructures
title_full_unstemmed Quantitative Mid-Infrared Plasmonic Biosensing on Scalable Graphene Nanostructures
title_sort Quantitative Mid-Infrared Plasmonic Biosensing on Scalable Graphene Nanostructures
dc.creator.none.fl_str_mv Bareza, Nestor Jr.
Wajs, Ewelina
Paulillo, Bruno
Tullila, Antti
Jaatinen, Hannakaisa
Milani, Roberto
Dore, Camilla
Mihi, Agustín
Nevanen, Tarja K.
Pruneri, Valerio
author Bareza, Nestor Jr.
author_facet Bareza, Nestor Jr.
Wajs, Ewelina
Paulillo, Bruno
Tullila, Antti
Jaatinen, Hannakaisa
Milani, Roberto
Dore, Camilla
Mihi, Agustín
Nevanen, Tarja K.
Pruneri, Valerio
author_role author
author2 Wajs, Ewelina
Paulillo, Bruno
Tullila, Antti
Jaatinen, Hannakaisa
Milani, Roberto
Dore, Camilla
Mihi, Agustín
Nevanen, Tarja K.
Pruneri, Valerio
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Fundación Privada Mir-Puig
Generalitat de Catalunya
Fundació Privada Cellex
VTT Technical Research Centre of Finland
Turku Bio Valley
Bareza, Nestor Jr. [0000-0002-0562-650X]
Wajs, Ewelina [0000-0002-6007-9692]
Paulillo, Bruno [0000-0002-6675-0141]
Milani, Roberto [0000-0002-7997-7847]
Dore, Camilla [0000-0002-4183-9609]
Mihi, Agustín [0000-0003-3821-7881]
Nevanen, Tarja K.[0000-0001-8964-1012]
Pruneri, Valerio [0000-0002-6425-9332]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Antibody
Graphene plasmonics
Mid-infrared biosensor
SEIRA
Vitamin B 12
topic Antibody
Graphene plasmonics
Mid-infrared biosensor
SEIRA
Vitamin B 12
description Graphene nanostructures, exhibiting tunable and nanoscale-confined mid-infrared (mid-IR) plasmons, prevail as a powerful spectroscopic platform for novel surface-enhanced molecular identification. Particularly, graphene shows exciting opportunities for biosensing applications due to its versatile functionalization methods with different biomolecular building blocks (e.g., enzymes, proteins, and DNA). Here, a quantitative bioassay based on the mid-IR localized surface plasmon resonance (LSPR) modulation in functionalized graphene nanostructures is demonstrated. Specifically, vitamin B12 (vB12) using the specific recognition elements on modified graphene nanoribbons (i.e., pyrene linkers via π − π stacking + anti-vB12 antibody fragments via amide bond) is detected. Different concentrations of vB12 spotted on an arrayed panel of a single chip are quantified by the graphene LSPR shifts, where a limit of detection (LOD) of 53.5 ng mL−1 is obtained. The upscaling potential of the bioassay using large area nanostructured graphene films produced by nanoimprinting 2D hole arrays is illustrated. The integration of quantitative bioassay with scalable graphene nanostructures shows promising routes of graphene-based mid-IR platforms toward prospective industrial applications.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/341289
https://api.elsevier.com/content/abstract/scopus_id/85142776536
url http://hdl.handle.net/10261/341289
https://api.elsevier.com/content/abstract/scopus_id/85142776536
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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info:eu-repo/grantAgreement/EC/H2020/881603
info:eu-repo/grantAgreement/EC/H2020/754510
info:eu-repo/grantAgreement/EC/H2020/665884
info:eu-repo/grantAgreement/AEI/Plan Estatal de investigación Científica y Técnica y de Innovación 2017-2020/CEX2019-000917-S
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Advanced Materials Interfaces
http://doi.org/10.1002/admi.202201699
Sí
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eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley-VCH
publisher.none.fl_str_mv Wiley-VCH
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
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spelling Quantitative Mid-Infrared Plasmonic Biosensing on Scalable Graphene NanostructuresBareza, Nestor Jr.Wajs, EwelinaPaulillo, BrunoTullila, AnttiJaatinen, HannakaisaMilani, RobertoDore, CamillaMihi, AgustínNevanen, Tarja K.Pruneri, ValerioAntibodyGraphene plasmonicsMid-infrared biosensorSEIRAVitamin B 12Graphene nanostructures, exhibiting tunable and nanoscale-confined mid-infrared (mid-IR) plasmons, prevail as a powerful spectroscopic platform for novel surface-enhanced molecular identification. Particularly, graphene shows exciting opportunities for biosensing applications due to its versatile functionalization methods with different biomolecular building blocks (e.g., enzymes, proteins, and DNA). Here, a quantitative bioassay based on the mid-IR localized surface plasmon resonance (LSPR) modulation in functionalized graphene nanostructures is demonstrated. Specifically, vitamin B12 (vB12) using the specific recognition elements on modified graphene nanoribbons (i.e., pyrene linkers via π − π stacking + anti-vB12 antibody fragments via amide bond) is detected. Different concentrations of vB12 spotted on an arrayed panel of a single chip are quantified by the graphene LSPR shifts, where a limit of detection (LOD) of 53.5 ng mL−1 is obtained. The upscaling potential of the bioassay using large area nanostructured graphene films produced by nanoimprinting 2D hole arrays is illustrated. The integration of quantitative bioassay with scalable graphene nanostructures shows promising routes of graphene-based mid-IR platforms toward prospective industrial applications.N.B. and E.W. contributed equally to this work. Tuula Kuurila and Salla Pentikäinen from VTT were thanked for technical assistance in antibody production and purification. Harri Siitari was thanked for support as a project manager during the anti-vB12 antibody development. The authors thank Daniel Martinez for help with AFM measurements. The research leading to these results has received funding from the H2020 Programme under Grant Agreement No. 881603 (Graphene Flagship). This project has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie Grant Agreement No. 754510. This project has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie Grant Agreement No. 665884. The authors acknowledge financial support from the Spanish Ministry of Economy and Competitiveness through the ‘Severo Ochoa’ Programme for Centres of Excellence in R&D (CEX2019-000910-S and CEX2019-000917-S) and project TUNA-SURF (PID2019-106892RB-I00) and PID2019-106860GB-I00 (HIGHN), Fundació Mir-Puig, and from Generalitat de Catalunya through the CERCA program, from AGAUR 2017 SGR 1634 and the Beatriu de Pinos-3 Postdoctoral Programme (BP3) under grant agreement ID 801370. This work was partially funded by CEX2019-000910-S [MCIN/ AEI/10.13039/501100011033], Fundació Cellex, Fundació Mir-Puig, and Generalitat de Catalunya through CERCA. Antibody discovery was funded by VTT Technical Research Centre of Finland and Development6 -project funded by Turku Science Park Oy, City of Turku, Turku Bio Valley Ltd.With funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000917-S).Peer reviewedWiley-VCHEuropean CommissionMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Fundación Privada Mir-PuigGeneralitat de CatalunyaFundació Privada CellexVTT Technical Research Centre of FinlandTurku Bio ValleyBareza, Nestor Jr. [0000-0002-0562-650X]Wajs, Ewelina [0000-0002-6007-9692]Paulillo, Bruno [0000-0002-6675-0141]Milani, Roberto [0000-0002-7997-7847]Dore, Camilla [0000-0002-4183-9609]Mihi, Agustín [0000-0003-3821-7881]Nevanen, Tarja K.[0000-0001-8964-1012]Pruneri, Valerio [0000-0002-6425-9332]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/341289https://api.elsevier.com/content/abstract/scopus_id/85142776536reponame:DIGITAL.CSIC. 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