Live Attenuated African Swine Fever Viruses as Ideal Tools to Dissect the Mechanisms Involved in Cross-Protection

African swine fever (ASF) has become the major threat for the global swine industry. Furthermore, the epidemiological situation of African swine fever virus (ASFV) in some endemic regions of Sub-Saharan Africa is worse than ever, with multiple virus strains and genotypes currently circulating in a g...

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Autores: López Fernandez, Elisabet|||0000-0003-4253-330X, van Heerden, Juanita|||0000-0002-3048-315X, Bosch Camós, Laia|||0000-0001-6245-4284, Accensi Alemany, Francesc|||0000-0001-9068-0377, Navas, María Jesús, López-Monteagudo, Paula, Argilaguet, Jordi|||0000-0001-5924-2774, Gallardo, Carmina|||0000-0003-3293-306X, Pina-Pedrero, Sonia|||0000-0001-5475-4770, Salas, Maria Luisa, Salt, Jeremy|||0000-0003-0404-6535, Rodriguez, Fernando|||0000-0001-7361-8408
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:253226
Acceso en línea:https://ddd.uab.cat/record/253226
https://dx.doi.org/urn:doi:10.3390/v12121474
Access Level:acceso abierto
Palabra clave:African swine fever virus
Live attenuate vaccine
Cross-protection
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spelling Live Attenuated African Swine Fever Viruses as Ideal Tools to Dissect the Mechanisms Involved in Cross-ProtectionLópez Fernandez, Elisabet|||0000-0003-4253-330Xvan Heerden, Juanita|||0000-0002-3048-315XBosch Camós, Laia|||0000-0001-6245-4284Accensi Alemany, Francesc|||0000-0001-9068-0377Navas, María JesúsLópez-Monteagudo, PaulaArgilaguet, Jordi|||0000-0001-5924-2774Gallardo, Carmina|||0000-0003-3293-306XPina-Pedrero, Sonia|||0000-0001-5475-4770Salas, Maria LuisaSalt, Jeremy|||0000-0003-0404-6535Rodriguez, Fernando|||0000-0001-7361-8408African swine fever virusLive attenuate vaccineCross-protectionAfrican swine fever (ASF) has become the major threat for the global swine industry. Furthermore, the epidemiological situation of African swine fever virus (ASFV) in some endemic regions of Sub-Saharan Africa is worse than ever, with multiple virus strains and genotypes currently circulating in a given area. Despite the recent advances on ASF vaccine development, there are no commercial vaccines yet, and most of the promising vaccine prototypes available today have been specifically designed to fight the genotype II strains currently circulating in Europe, Asia, and Oceania. Previous results from our laboratory have demonstrated the ability of BA71∆CD2, a recombinant LAV lacking CD2v, to confer protection against homologous (BA71) and heterologous genotype I (E75) and genotype II (Georgia2007/01) ASFV strains, both belonging to same clade (clade C). Here, we extend these results using BA71∆CD2 as a tool trying to understand ASFV cross-protection, using phylogenetically distant ASFV strains. We first observed that five out of six (83.3%) of the pigs immunized once with 10 6 PFU of BA71∆CD2 survived the tick-bite challenge using Ornithodoros sp. soft ticks naturally infected with RSA/11/2017 strain (genotype XIX, clade D). Second, only two out of six (33.3%) survived the challenge with Ken06.Bus (genotype IX, clade A), which is phylogenetically more distant to BA71∆CD2 than the RSA/11/2017 strain. On the other hand, homologous prime-boosting with BA71∆CD2 only improved the survival rate to 50% after Ken06.Bus challenge, all suffering mild ASF-compatible clinical signs, while 100% of the pigs immunized with BA71∆CD2 and boosted with the parental BA71 virulent strain survived the lethal challenge with Ken06.Bus, without almost no clinical signs of the disease. Our results confirm that cross-protection is a multifactorial phenomenon that not only depends on sequence similarity. We believe that understanding this complex phenomenon will be useful for designing future vaccines for ASF-endemic areas. 22020-01-0120202020-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/253226https://dx.doi.org/urn:doi:10.3390/v12121474reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 AGL2016-78160-C2-1-RAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2019-107616RB-I00open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2532262026-06-06T12:50:31Z
dc.title.none.fl_str_mv Live Attenuated African Swine Fever Viruses as Ideal Tools to Dissect the Mechanisms Involved in Cross-Protection
title Live Attenuated African Swine Fever Viruses as Ideal Tools to Dissect the Mechanisms Involved in Cross-Protection
spellingShingle Live Attenuated African Swine Fever Viruses as Ideal Tools to Dissect the Mechanisms Involved in Cross-Protection
López Fernandez, Elisabet|||0000-0003-4253-330X
African swine fever virus
Live attenuate vaccine
Cross-protection
title_short Live Attenuated African Swine Fever Viruses as Ideal Tools to Dissect the Mechanisms Involved in Cross-Protection
title_full Live Attenuated African Swine Fever Viruses as Ideal Tools to Dissect the Mechanisms Involved in Cross-Protection
title_fullStr Live Attenuated African Swine Fever Viruses as Ideal Tools to Dissect the Mechanisms Involved in Cross-Protection
title_full_unstemmed Live Attenuated African Swine Fever Viruses as Ideal Tools to Dissect the Mechanisms Involved in Cross-Protection
title_sort Live Attenuated African Swine Fever Viruses as Ideal Tools to Dissect the Mechanisms Involved in Cross-Protection
dc.creator.none.fl_str_mv López Fernandez, Elisabet|||0000-0003-4253-330X
van Heerden, Juanita|||0000-0002-3048-315X
Bosch Camós, Laia|||0000-0001-6245-4284
Accensi Alemany, Francesc|||0000-0001-9068-0377
Navas, María Jesús
López-Monteagudo, Paula
Argilaguet, Jordi|||0000-0001-5924-2774
Gallardo, Carmina|||0000-0003-3293-306X
Pina-Pedrero, Sonia|||0000-0001-5475-4770
Salas, Maria Luisa
Salt, Jeremy|||0000-0003-0404-6535
Rodriguez, Fernando|||0000-0001-7361-8408
author López Fernandez, Elisabet|||0000-0003-4253-330X
author_facet López Fernandez, Elisabet|||0000-0003-4253-330X
van Heerden, Juanita|||0000-0002-3048-315X
Bosch Camós, Laia|||0000-0001-6245-4284
Accensi Alemany, Francesc|||0000-0001-9068-0377
Navas, María Jesús
López-Monteagudo, Paula
Argilaguet, Jordi|||0000-0001-5924-2774
Gallardo, Carmina|||0000-0003-3293-306X
Pina-Pedrero, Sonia|||0000-0001-5475-4770
Salas, Maria Luisa
Salt, Jeremy|||0000-0003-0404-6535
Rodriguez, Fernando|||0000-0001-7361-8408
author_role author
author2 van Heerden, Juanita|||0000-0002-3048-315X
Bosch Camós, Laia|||0000-0001-6245-4284
Accensi Alemany, Francesc|||0000-0001-9068-0377
Navas, María Jesús
López-Monteagudo, Paula
Argilaguet, Jordi|||0000-0001-5924-2774
Gallardo, Carmina|||0000-0003-3293-306X
Pina-Pedrero, Sonia|||0000-0001-5475-4770
Salas, Maria Luisa
Salt, Jeremy|||0000-0003-0404-6535
Rodriguez, Fernando|||0000-0001-7361-8408
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv African swine fever virus
Live attenuate vaccine
Cross-protection
topic African swine fever virus
Live attenuate vaccine
Cross-protection
description African swine fever (ASF) has become the major threat for the global swine industry. Furthermore, the epidemiological situation of African swine fever virus (ASFV) in some endemic regions of Sub-Saharan Africa is worse than ever, with multiple virus strains and genotypes currently circulating in a given area. Despite the recent advances on ASF vaccine development, there are no commercial vaccines yet, and most of the promising vaccine prototypes available today have been specifically designed to fight the genotype II strains currently circulating in Europe, Asia, and Oceania. Previous results from our laboratory have demonstrated the ability of BA71∆CD2, a recombinant LAV lacking CD2v, to confer protection against homologous (BA71) and heterologous genotype I (E75) and genotype II (Georgia2007/01) ASFV strains, both belonging to same clade (clade C). Here, we extend these results using BA71∆CD2 as a tool trying to understand ASFV cross-protection, using phylogenetically distant ASFV strains. We first observed that five out of six (83.3%) of the pigs immunized once with 10 6 PFU of BA71∆CD2 survived the tick-bite challenge using Ornithodoros sp. soft ticks naturally infected with RSA/11/2017 strain (genotype XIX, clade D). Second, only two out of six (33.3%) survived the challenge with Ken06.Bus (genotype IX, clade A), which is phylogenetically more distant to BA71∆CD2 than the RSA/11/2017 strain. On the other hand, homologous prime-boosting with BA71∆CD2 only improved the survival rate to 50% after Ken06.Bus challenge, all suffering mild ASF-compatible clinical signs, while 100% of the pigs immunized with BA71∆CD2 and boosted with the parental BA71 virulent strain survived the lethal challenge with Ken06.Bus, without almost no clinical signs of the disease. Our results confirm that cross-protection is a multifactorial phenomenon that not only depends on sequence similarity. We believe that understanding this complex phenomenon will be useful for designing future vaccines for ASF-endemic areas.
publishDate 2020
dc.date.none.fl_str_mv 2
2020-01-01
2020
2020-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/253226
https://dx.doi.org/urn:doi:10.3390/v12121474
url https://ddd.uab.cat/record/253226
https://dx.doi.org/urn:doi:10.3390/v12121474
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 AGL2016-78160-C2-1-R
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2019-107616RB-I00
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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