The relevance of the UPS in fatty liver graft preservation: A new approach for IGL-1 and HTK solutions

The 26S proteasome is the central proteolytic machinery of the ubiquitin proteasome system (UPS), which is involved in the degradation of ubiquitinated protein substrates. Recently, UPS inhibition has been shown to be a key factor in fatty liver graft preservation during organ cold storage using Uni...

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Autores: Panisello-Roselló, Arnau, Verde, Eva, Zaouali, Mohamed A., Flores, Marta, Alva, Norma, López, Alexandre, Folch-Puy, Emma, Carbonell, Teresa, Hotter, Georgina, Adam, René, Roselló-Catafau, Joan
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/176579
Acesso em linha:http://hdl.handle.net/10261/176579
Access Level:acceso abierto
Palavra-chave:Ubiquitin proteasome system
Cold ischemic injury
Fatty liver preservation
IGL-1
HTK
ATP
AMPK and nitric oxide
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spelling The relevance of the UPS in fatty liver graft preservation: A new approach for IGL-1 and HTK solutionsPanisello-Roselló, ArnauVerde, EvaZaouali, Mohamed A.Flores, MartaAlva, NormaLópez, AlexandreFolch-Puy, EmmaCarbonell, TeresaHotter, GeorginaAdam, RenéRoselló-Catafau, JoanUbiquitin proteasome systemCold ischemic injuryFatty liver preservationIGL-1HTKATPAMPK and nitric oxideThe 26S proteasome is the central proteolytic machinery of the ubiquitin proteasome system (UPS), which is involved in the degradation of ubiquitinated protein substrates. Recently, UPS inhibition has been shown to be a key factor in fatty liver graft preservation during organ cold storage using University of Wisconsin solution (UW) and Institute Georges Lopez (IGL-1) solutions. However, the merits of IGL-1 and histidine-tryptophan-ketoglutarate (HTK) solutions for fatty liver preservation have not been compared. Fatty liver grafts from obese Zücker rats were preserved for 24 h at 4 ◦C. Aspartate aminotransferase and alanine aminotransferase (AST/ALT), glutamate dehydrogenase (GLDH), ATP, adenosine monophosphate protein kinase (AMPK), e-NOS, proteasome activity and liver polyubiquitinated proteins were determined. IGL-1 solution prevented ATP breakdown during cold-storage preservation of steatotic livers to a greater extent than HTK solution. There were concomitant increases in AMPK activation, e-NOS (endothelial NOS (NO synthase)) expression and UPS inhibition. UPS activity is closely related to the composition of the solution used to preserve the organ. IGL-1 solution provided significantly better protection against ischemia-reperfusion for cold-stored fatty liver grafts than HTK solution. The effect is exerted through the activation of the protective AMPK signaling pathway, an increase in e-NOS expression and a dysregulation of the UPS.This work was supported by Instituto de Salud Carlos III (ISCIII) through the FIS project PI12/0056 co-funded by FEDER from Regional Development European Funds (European Union).Peer reviewedMultidisciplinary Digital Publishing InstituteInstituto de Salud Carlos IIIEuropean CommissionCarbonell, Teresa [0000-0002-7131-3667]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/176579reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.3390/ijms18112287Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1765792026-05-22T06:33:51Z
dc.title.none.fl_str_mv The relevance of the UPS in fatty liver graft preservation: A new approach for IGL-1 and HTK solutions
title The relevance of the UPS in fatty liver graft preservation: A new approach for IGL-1 and HTK solutions
spellingShingle The relevance of the UPS in fatty liver graft preservation: A new approach for IGL-1 and HTK solutions
Panisello-Roselló, Arnau
Ubiquitin proteasome system
Cold ischemic injury
Fatty liver preservation
IGL-1
HTK
ATP
AMPK and nitric oxide
title_short The relevance of the UPS in fatty liver graft preservation: A new approach for IGL-1 and HTK solutions
title_full The relevance of the UPS in fatty liver graft preservation: A new approach for IGL-1 and HTK solutions
title_fullStr The relevance of the UPS in fatty liver graft preservation: A new approach for IGL-1 and HTK solutions
title_full_unstemmed The relevance of the UPS in fatty liver graft preservation: A new approach for IGL-1 and HTK solutions
title_sort The relevance of the UPS in fatty liver graft preservation: A new approach for IGL-1 and HTK solutions
dc.creator.none.fl_str_mv Panisello-Roselló, Arnau
Verde, Eva
Zaouali, Mohamed A.
Flores, Marta
Alva, Norma
López, Alexandre
Folch-Puy, Emma
Carbonell, Teresa
Hotter, Georgina
Adam, René
Roselló-Catafau, Joan
author Panisello-Roselló, Arnau
author_facet Panisello-Roselló, Arnau
Verde, Eva
Zaouali, Mohamed A.
Flores, Marta
Alva, Norma
López, Alexandre
Folch-Puy, Emma
Carbonell, Teresa
Hotter, Georgina
Adam, René
Roselló-Catafau, Joan
author_role author
author2 Verde, Eva
Zaouali, Mohamed A.
Flores, Marta
Alva, Norma
López, Alexandre
Folch-Puy, Emma
Carbonell, Teresa
Hotter, Georgina
Adam, René
Roselló-Catafau, Joan
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
European Commission
Carbonell, Teresa [0000-0002-7131-3667]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Ubiquitin proteasome system
Cold ischemic injury
Fatty liver preservation
IGL-1
HTK
ATP
AMPK and nitric oxide
topic Ubiquitin proteasome system
Cold ischemic injury
Fatty liver preservation
IGL-1
HTK
ATP
AMPK and nitric oxide
description The 26S proteasome is the central proteolytic machinery of the ubiquitin proteasome system (UPS), which is involved in the degradation of ubiquitinated protein substrates. Recently, UPS inhibition has been shown to be a key factor in fatty liver graft preservation during organ cold storage using University of Wisconsin solution (UW) and Institute Georges Lopez (IGL-1) solutions. However, the merits of IGL-1 and histidine-tryptophan-ketoglutarate (HTK) solutions for fatty liver preservation have not been compared. Fatty liver grafts from obese Zücker rats were preserved for 24 h at 4 ◦C. Aspartate aminotransferase and alanine aminotransferase (AST/ALT), glutamate dehydrogenase (GLDH), ATP, adenosine monophosphate protein kinase (AMPK), e-NOS, proteasome activity and liver polyubiquitinated proteins were determined. IGL-1 solution prevented ATP breakdown during cold-storage preservation of steatotic livers to a greater extent than HTK solution. There were concomitant increases in AMPK activation, e-NOS (endothelial NOS (NO synthase)) expression and UPS inhibition. UPS activity is closely related to the composition of the solution used to preserve the organ. IGL-1 solution provided significantly better protection against ischemia-reperfusion for cold-stored fatty liver grafts than HTK solution. The effect is exerted through the activation of the protective AMPK signaling pathway, an increase in e-NOS expression and a dysregulation of the UPS.
publishDate 2017
dc.date.none.fl_str_mv 2017
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/176579
url http://hdl.handle.net/10261/176579
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3390/ijms18112287
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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