Insights into the structure and nanomechanics of a quatsome membrane by force spectroscopy measurements and molecular simulation

Quatsomes (QS) are unilamellar nanovesicles constituted by quaternary ammonium surfactants and sterols in defined molar ratios. Unlike conventional liposomes, QS are stable upon long storage such as for several years, they show outstanding vesicle-to-vesicle homogeneity regarding size and lamellarit...

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Autores: Gumí Audenis, Berta, Illa Tuset, Silvia, Grimaldi, Natascia, Pasquina Lemonche, Laia, Ferrer Tasies, Lídia, Sanz Carrasco, Fausto, Veciana, Jaume, Ratera, Imma, Faraudo, Jordi, Ventosa, Nora, Giannotti, Marina Inés
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/147277
Acceso en línea:https://hdl.handle.net/2445/147277
Access Level:acceso abierto
Palabra clave:Dinàmica molecular
Nanotecnologia
Membranes (Biologia)
Microscòpia de força atòmica
Molecular dynamics
Nanotechnology
Membranes (Biology)
Atomic force microscopy
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spelling Insights into the structure and nanomechanics of a quatsome membrane by force spectroscopy measurements and molecular simulationGumí Audenis, BertaIlla Tuset, SilviaGrimaldi, NatasciaPasquina Lemonche, LaiaFerrer Tasies, LídiaSanz Carrasco, FaustoVeciana, JaumeRatera, ImmaFaraudo, JordiVentosa, NoraGiannotti, Marina InésDinàmica molecularNanotecnologiaMembranes (Biologia)Microscòpia de força atòmicaMolecular dynamicsNanotechnologyMembranes (Biology)Atomic force microscopyQuatsomes (QS) are unilamellar nanovesicles constituted by quaternary ammonium surfactants and sterols in defined molar ratios. Unlike conventional liposomes, QS are stable upon long storage such as for several years, they show outstanding vesicle-to-vesicle homogeneity regarding size and lamellarity, and they have the structural and physicochemical requirements to be a potential platform for site-specific delivery of hydrophilic and lipophilic molecules. Knowing in detail the structure and mechanical properties of the QS membrane is of great importance for the design of deformable and flexible nanovesicle alternatives, highly pursued in nanomedicine applications such as the transdermal administration route. In this work, we report the first study on the detailed structure of the cholesterol : CTAB QS membrane at the nanoscale, using atomic force microscopy (AFM) and spectroscopy (AFM-FS) in a controlled liquid environment (ionic medium and temperature) to assess the topography of supported QS membranes (SQMs) and to evaluate the local membrane mechanics. We further perform molecular dynamics (MD) simulations to provide an atomistic interpretation of the obtained results. Our results are direct evidence of the bilayer nature of the QS membrane, with characteristics of a fluid-like membrane, compact and homogeneous in composition, and with structural and mechanical properties that depend on the surrounding environment. We show how ions alter the lateral packing, modifying the membrane mechanics. We observe that according to the ionic environment and temperature, different domains may coexist in the QS membranes, ascribed to variations in molecular tilt angles. Our results indicate that QS membrane properties may be easily tuned by altering the lateral interactions with either different environmental ions or counterions.Royal Society of Chemistry2020202020182020info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion11 p.application/pdfapplication/pdfhttps://hdl.handle.net/2445/147277Articles publicats en revistes (Ciència dels Materials i Química Física)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1039/C8NR07110ANanoscale, 2018, vol. 10, num. 48, p. 23001-23011https://doi.org/10.1039/C8NR07110A(c) Gumí Audenis, Berta et al., 2018info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1472772026-05-29T05:05:01Z
dc.title.none.fl_str_mv Insights into the structure and nanomechanics of a quatsome membrane by force spectroscopy measurements and molecular simulation
title Insights into the structure and nanomechanics of a quatsome membrane by force spectroscopy measurements and molecular simulation
spellingShingle Insights into the structure and nanomechanics of a quatsome membrane by force spectroscopy measurements and molecular simulation
Gumí Audenis, Berta
Dinàmica molecular
Nanotecnologia
Membranes (Biologia)
Microscòpia de força atòmica
Molecular dynamics
Nanotechnology
Membranes (Biology)
Atomic force microscopy
title_short Insights into the structure and nanomechanics of a quatsome membrane by force spectroscopy measurements and molecular simulation
title_full Insights into the structure and nanomechanics of a quatsome membrane by force spectroscopy measurements and molecular simulation
title_fullStr Insights into the structure and nanomechanics of a quatsome membrane by force spectroscopy measurements and molecular simulation
title_full_unstemmed Insights into the structure and nanomechanics of a quatsome membrane by force spectroscopy measurements and molecular simulation
title_sort Insights into the structure and nanomechanics of a quatsome membrane by force spectroscopy measurements and molecular simulation
dc.creator.none.fl_str_mv Gumí Audenis, Berta
Illa Tuset, Silvia
Grimaldi, Natascia
Pasquina Lemonche, Laia
Ferrer Tasies, Lídia
Sanz Carrasco, Fausto
Veciana, Jaume
Ratera, Imma
Faraudo, Jordi
Ventosa, Nora
Giannotti, Marina Inés
author Gumí Audenis, Berta
author_facet Gumí Audenis, Berta
Illa Tuset, Silvia
Grimaldi, Natascia
Pasquina Lemonche, Laia
Ferrer Tasies, Lídia
Sanz Carrasco, Fausto
Veciana, Jaume
Ratera, Imma
Faraudo, Jordi
Ventosa, Nora
Giannotti, Marina Inés
author_role author
author2 Illa Tuset, Silvia
Grimaldi, Natascia
Pasquina Lemonche, Laia
Ferrer Tasies, Lídia
Sanz Carrasco, Fausto
Veciana, Jaume
Ratera, Imma
Faraudo, Jordi
Ventosa, Nora
Giannotti, Marina Inés
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Dinàmica molecular
Nanotecnologia
Membranes (Biologia)
Microscòpia de força atòmica
Molecular dynamics
Nanotechnology
Membranes (Biology)
Atomic force microscopy
topic Dinàmica molecular
Nanotecnologia
Membranes (Biologia)
Microscòpia de força atòmica
Molecular dynamics
Nanotechnology
Membranes (Biology)
Atomic force microscopy
description Quatsomes (QS) are unilamellar nanovesicles constituted by quaternary ammonium surfactants and sterols in defined molar ratios. Unlike conventional liposomes, QS are stable upon long storage such as for several years, they show outstanding vesicle-to-vesicle homogeneity regarding size and lamellarity, and they have the structural and physicochemical requirements to be a potential platform for site-specific delivery of hydrophilic and lipophilic molecules. Knowing in detail the structure and mechanical properties of the QS membrane is of great importance for the design of deformable and flexible nanovesicle alternatives, highly pursued in nanomedicine applications such as the transdermal administration route. In this work, we report the first study on the detailed structure of the cholesterol : CTAB QS membrane at the nanoscale, using atomic force microscopy (AFM) and spectroscopy (AFM-FS) in a controlled liquid environment (ionic medium and temperature) to assess the topography of supported QS membranes (SQMs) and to evaluate the local membrane mechanics. We further perform molecular dynamics (MD) simulations to provide an atomistic interpretation of the obtained results. Our results are direct evidence of the bilayer nature of the QS membrane, with characteristics of a fluid-like membrane, compact and homogeneous in composition, and with structural and mechanical properties that depend on the surrounding environment. We show how ions alter the lateral packing, modifying the membrane mechanics. We observe that according to the ionic environment and temperature, different domains may coexist in the QS membranes, ascribed to variations in molecular tilt angles. Our results indicate that QS membrane properties may be easily tuned by altering the lateral interactions with either different environmental ions or counterions.
publishDate 2018
dc.date.none.fl_str_mv 2018
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/147277
url https://hdl.handle.net/2445/147277
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1039/C8NR07110A
Nanoscale, 2018, vol. 10, num. 48, p. 23001-23011
https://doi.org/10.1039/C8NR07110A
dc.rights.none.fl_str_mv (c) Gumí Audenis, Berta et al., 2018
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Gumí Audenis, Berta et al., 2018
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11 p.
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv Articles publicats en revistes (Ciència dels Materials i Química Física)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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