Immunoreagents and competitive assays to fludioxonil

Fludioxonil is a new-generation fungicide widely used for postharvest fruit protection. The aim of this study was to produce hitherto unreported immunoreagents for Fludioxonil analysis by immunoassay. Derivatives of this agrochemical were synthesized with different linker tethering sites. Those func...

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Autores: Abad Fuentes, Antonio, Agulló, Consuelo, Esteve Turrillas, Francesc A., Abad Somovilla, Antonio, Mercader Badia, Josep Vicent
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2014
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/102975
Acceso en línea:http://hdl.handle.net/10261/102975
Access Level:acceso abierto
Palabra clave:Antibodies
Rapid method
Hapten
Active ester
Postharvest fungicide
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spelling Immunoreagents and competitive assays to fludioxonilAbad Fuentes, AntonioAgulló, ConsueloEsteve Turrillas, Francesc A.Abad Somovilla, AntonioMercader Badia, Josep VicentAntibodiesRapid methodHaptenActive esterPostharvest fungicideFludioxonil is a new-generation fungicide widely used for postharvest fruit protection. The aim of this study was to produce hitherto unreported immunoreagents for Fludioxonil analysis by immunoassay. Derivatives of this agrochemical were synthesized with different linker tethering sites. Those functionalized haptens were activated, and the purified active esters were efficiently conjugated to different carrier proteins for immunogen and assay antigen preparation. Antibodies to Fludioxonil were raised in rabbits, and their selectivity and affinity were characterized, revealing the significance of the linker. Those antibodies were evaluated using homologous and heterologous conjugates by direct and indirect competitive ELISA formats. Finally, a pair of immunoreagents was identified showing an IC50 value for Fludioxonil of 5.7 μg/L. © 2014 .This work was supported by the Spanish Ministerio de Ciencia e Innovación (MICINN) (AGL2009‐12940‐C02‐01/02/ALI) and cofinanced by FEDER funds. F.A.E.‐T. and J.V.M. were hired by CSIC with postdoctoral contracts, the former under the JAE‐doc program and the latter under the Ramón y Cajal program, both cofinanced by MICINN and by the European Social Fund (ESF).Peer ReviewedAmerican Chemical Society2014201420142014info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/102975reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglésinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1029752026-05-22T06:33:51Z
dc.title.none.fl_str_mv Immunoreagents and competitive assays to fludioxonil
title Immunoreagents and competitive assays to fludioxonil
spellingShingle Immunoreagents and competitive assays to fludioxonil
Abad Fuentes, Antonio
Antibodies
Rapid method
Hapten
Active ester
Postharvest fungicide
title_short Immunoreagents and competitive assays to fludioxonil
title_full Immunoreagents and competitive assays to fludioxonil
title_fullStr Immunoreagents and competitive assays to fludioxonil
title_full_unstemmed Immunoreagents and competitive assays to fludioxonil
title_sort Immunoreagents and competitive assays to fludioxonil
dc.creator.none.fl_str_mv Abad Fuentes, Antonio
Agulló, Consuelo
Esteve Turrillas, Francesc A.
Abad Somovilla, Antonio
Mercader Badia, Josep Vicent
author Abad Fuentes, Antonio
author_facet Abad Fuentes, Antonio
Agulló, Consuelo
Esteve Turrillas, Francesc A.
Abad Somovilla, Antonio
Mercader Badia, Josep Vicent
author_role author
author2 Agulló, Consuelo
Esteve Turrillas, Francesc A.
Abad Somovilla, Antonio
Mercader Badia, Josep Vicent
author2_role author
author
author
author
dc.subject.none.fl_str_mv Antibodies
Rapid method
Hapten
Active ester
Postharvest fungicide
topic Antibodies
Rapid method
Hapten
Active ester
Postharvest fungicide
description Fludioxonil is a new-generation fungicide widely used for postharvest fruit protection. The aim of this study was to produce hitherto unreported immunoreagents for Fludioxonil analysis by immunoassay. Derivatives of this agrochemical were synthesized with different linker tethering sites. Those functionalized haptens were activated, and the purified active esters were efficiently conjugated to different carrier proteins for immunogen and assay antigen preparation. Antibodies to Fludioxonil were raised in rabbits, and their selectivity and affinity were characterized, revealing the significance of the linker. Those antibodies were evaluated using homologous and heterologous conjugates by direct and indirect competitive ELISA formats. Finally, a pair of immunoreagents was identified showing an IC50 value for Fludioxonil of 5.7 μg/L. © 2014 .
publishDate 2014
dc.date.none.fl_str_mv 2014
2014
2014
2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/102975
url http://hdl.handle.net/10261/102975
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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