Development of a novel near-infrared fluorescent probe based on nile blue for the detection of leucine aminopeptidase as a cancer biomarker
We report herein the design and synthesis of a near-infrared fluorescent probe (NB-SO3-Leu) derived from the Nile Blue (NB) fluorophore scaffold, modified with sulfonic groups to improve solubility and biocompatibility. The mechanism of action of the NB-SO3-Leu probe was successfully validated in th...
| Authors: | , , , , , , , , |
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| Format: | article |
| Status: | Published version |
| Publication Date: | 2025 |
| Country: | España |
| Institution: | Centro de Investigación Principe Felipe (CIPF) |
| Repository: | r-CIPF. Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF) |
| OAI Identifier: | oai:cipf.fundanetsuite.com:p4526 |
| Online Access: | https://cipf.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=4526 |
| Access Level: | Open access |
| Keyword: | Near-infrared fluorescent probe Cancer biomarker Leucine aminopeptidase Zwitterionic compounds Biocompatibility |
| Summary: | We report herein the design and synthesis of a near-infrared fluorescent probe (NB-SO3-Leu) derived from the Nile Blue (NB) fluorophore scaffold, modified with sulfonic groups to improve solubility and biocompatibility. The mechanism of action of the NB-SO3-Leu probe was successfully validated in the presence of leucine aminopeptidase (LAP), a widely known cancer biomarker. The fluorescence signal of NB-SO3-Leu, which was initially low, increased 4-fold after incubation with the LAP enzyme after 15 min. In addition, the NB-SO3-Leu probe was validated in vitro in 4T1 (mouse mammary carcinoma cells), HeLa (human cervical cancer cells), and SK-Mel-103 (human melanoma cells) with high endogenous LAP levels. This work aims to advance in the development of a new generation of reliable and sensitive cancer detection methods. |
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