Insulin-induced recruitment of glucose transporter 4 (GLUT4) and GLUT1 in isolated rat cardiac myocytes. Evidence of the existence of different intracellular GLUT4 vesicle populations

Using isolated rat cardiomyocytes we have examined: 1) the effect of insulin on the cellular distribution of glucose transporter 4 (GLUT4) and GLUT1, 2) the total amount of these transporters, and 3) the co-localization of GLUT4, GLUT1, and secretory carrier membrane proteins (SCAMPs) in intracellul...

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Autores: Fischer, Yvan, Thomas, Julia, Sevilla, Lidia, Muñoz Moruno, Purificación, Becker, Christoph, Holman, Geoffrey, Kozka, Izabela J., Palacín Prieto, Manuel, Testar, Xavier, Kammermeier, Helmut, Zorzano Olarte, Antonio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:1997
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/177124
Acceso en línea:https://hdl.handle.net/2445/177124
Access Level:acceso abierto
Palabra clave:Hipoglucèmia
Insulina
Farmacologia
Monosacàrids
Proteïnes portadores
Miocardi
Hypoglycemia
Insulin
Pharmacology
Monosaccharides
Carrier proteins
Myocardium
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oai_identifier_str oai:recercat.cat:2445/177124
network_acronym_str ES
network_name_str España
repository_id_str
spelling Insulin-induced recruitment of glucose transporter 4 (GLUT4) and GLUT1 in isolated rat cardiac myocytes. Evidence of the existence of different intracellular GLUT4 vesicle populationsFischer, YvanThomas, JuliaSevilla, LidiaMuñoz Moruno, PurificaciónBecker, ChristophHolman, GeoffreyKozka, Izabela J.Palacín Prieto, ManuelTestar, XavierKammermeier, HelmutZorzano Olarte, AntonioHipoglucèmiaInsulinaFarmacologiaMonosacàridsProteïnes portadoresMiocardiHypoglycemiaInsulinPharmacologyMonosaccharidesCarrier proteinsMyocardiumUsing isolated rat cardiomyocytes we have examined: 1) the effect of insulin on the cellular distribution of glucose transporter 4 (GLUT4) and GLUT1, 2) the total amount of these transporters, and 3) the co-localization of GLUT4, GLUT1, and secretory carrier membrane proteins (SCAMPs) in intracellular membranes. Insulin induced 5.7- and 2.7-fold increases in GLUT4 and GLUT1 at the cell surface, respectively, as determined by the nonpermeant photoaffinity label [3H]2-N-[4(1-azi-2,2,2-trifluoroethyl)benzoyl]-1, 3-bis-(D-mannos-4-yloxy)propyl-2-amine. The total amount of GLUT1, as determined by quantitative Western blot analysis of cell homogenates, was found to represent a substantial fraction ( approximately 30%) of the total glucose transporter content. Intracellular GLUT4-containing vesicles were immunoisolated from low density microsomes by using monoclonal anti-GLUT4 (1F8) or anti-SCAMP antibodies (3F8) coupled to either agarose or acrylamide. With these different immunoisolation conditions two GLUT4 membrane pools were found in nonstimulated cells: one pool with a high proportion of GLUT4 and a low content in GLUT1 and SCAMP 39 (pool 1) and a second GLUT4 pool with a high content of GLUT1 and SCAMP 39 (pool 2). The existence of pool 1 was confirmed by immunotitration of intracellular GLUT4 membranes with 1F8-acrylamide. Acute insulin treatment caused the depletion of GLUT4 in both pools and of GLUT1 and SCAMP 39 in pool 2. In conclusion: 1) GLUT4 is the major glucose transporter to be recruited to the surface of cardiomyocytes in response to insulin; 2) these cells express a high level of GLUT1; and 3) intracellular GLUT4-containing vesicles consist of at least two populations, which is compatible with recently proposed models of GLUT4 trafficking in adipocytes.American Society for Biochemistry and Molecular Biology2021202119972021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion8 p.application/pdfhttps://hdl.handle.net/2445/177124Articles publicats en revistes (Ciències Fisiològiques)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1074/jbc.272.11.7085Journal of Biological Chemistry, 1997, vol. 272, num. 11, p. 7085-7092https://doi.org/10.1074/jbc.272.11.7085(c) American Society for Biochemistry and Molecular Biology, 1997info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1771242026-05-29T05:05:01Z
dc.title.none.fl_str_mv Insulin-induced recruitment of glucose transporter 4 (GLUT4) and GLUT1 in isolated rat cardiac myocytes. Evidence of the existence of different intracellular GLUT4 vesicle populations
title Insulin-induced recruitment of glucose transporter 4 (GLUT4) and GLUT1 in isolated rat cardiac myocytes. Evidence of the existence of different intracellular GLUT4 vesicle populations
spellingShingle Insulin-induced recruitment of glucose transporter 4 (GLUT4) and GLUT1 in isolated rat cardiac myocytes. Evidence of the existence of different intracellular GLUT4 vesicle populations
Fischer, Yvan
Hipoglucèmia
Insulina
Farmacologia
Monosacàrids
Proteïnes portadores
Miocardi
Hypoglycemia
Insulin
Pharmacology
Monosaccharides
Carrier proteins
Myocardium
title_short Insulin-induced recruitment of glucose transporter 4 (GLUT4) and GLUT1 in isolated rat cardiac myocytes. Evidence of the existence of different intracellular GLUT4 vesicle populations
title_full Insulin-induced recruitment of glucose transporter 4 (GLUT4) and GLUT1 in isolated rat cardiac myocytes. Evidence of the existence of different intracellular GLUT4 vesicle populations
title_fullStr Insulin-induced recruitment of glucose transporter 4 (GLUT4) and GLUT1 in isolated rat cardiac myocytes. Evidence of the existence of different intracellular GLUT4 vesicle populations
title_full_unstemmed Insulin-induced recruitment of glucose transporter 4 (GLUT4) and GLUT1 in isolated rat cardiac myocytes. Evidence of the existence of different intracellular GLUT4 vesicle populations
title_sort Insulin-induced recruitment of glucose transporter 4 (GLUT4) and GLUT1 in isolated rat cardiac myocytes. Evidence of the existence of different intracellular GLUT4 vesicle populations
dc.creator.none.fl_str_mv Fischer, Yvan
Thomas, Julia
Sevilla, Lidia
Muñoz Moruno, Purificación
Becker, Christoph
Holman, Geoffrey
Kozka, Izabela J.
Palacín Prieto, Manuel
Testar, Xavier
Kammermeier, Helmut
Zorzano Olarte, Antonio
author Fischer, Yvan
author_facet Fischer, Yvan
Thomas, Julia
Sevilla, Lidia
Muñoz Moruno, Purificación
Becker, Christoph
Holman, Geoffrey
Kozka, Izabela J.
Palacín Prieto, Manuel
Testar, Xavier
Kammermeier, Helmut
Zorzano Olarte, Antonio
author_role author
author2 Thomas, Julia
Sevilla, Lidia
Muñoz Moruno, Purificación
Becker, Christoph
Holman, Geoffrey
Kozka, Izabela J.
Palacín Prieto, Manuel
Testar, Xavier
Kammermeier, Helmut
Zorzano Olarte, Antonio
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Hipoglucèmia
Insulina
Farmacologia
Monosacàrids
Proteïnes portadores
Miocardi
Hypoglycemia
Insulin
Pharmacology
Monosaccharides
Carrier proteins
Myocardium
topic Hipoglucèmia
Insulina
Farmacologia
Monosacàrids
Proteïnes portadores
Miocardi
Hypoglycemia
Insulin
Pharmacology
Monosaccharides
Carrier proteins
Myocardium
description Using isolated rat cardiomyocytes we have examined: 1) the effect of insulin on the cellular distribution of glucose transporter 4 (GLUT4) and GLUT1, 2) the total amount of these transporters, and 3) the co-localization of GLUT4, GLUT1, and secretory carrier membrane proteins (SCAMPs) in intracellular membranes. Insulin induced 5.7- and 2.7-fold increases in GLUT4 and GLUT1 at the cell surface, respectively, as determined by the nonpermeant photoaffinity label [3H]2-N-[4(1-azi-2,2,2-trifluoroethyl)benzoyl]-1, 3-bis-(D-mannos-4-yloxy)propyl-2-amine. The total amount of GLUT1, as determined by quantitative Western blot analysis of cell homogenates, was found to represent a substantial fraction ( approximately 30%) of the total glucose transporter content. Intracellular GLUT4-containing vesicles were immunoisolated from low density microsomes by using monoclonal anti-GLUT4 (1F8) or anti-SCAMP antibodies (3F8) coupled to either agarose or acrylamide. With these different immunoisolation conditions two GLUT4 membrane pools were found in nonstimulated cells: one pool with a high proportion of GLUT4 and a low content in GLUT1 and SCAMP 39 (pool 1) and a second GLUT4 pool with a high content of GLUT1 and SCAMP 39 (pool 2). The existence of pool 1 was confirmed by immunotitration of intracellular GLUT4 membranes with 1F8-acrylamide. Acute insulin treatment caused the depletion of GLUT4 in both pools and of GLUT1 and SCAMP 39 in pool 2. In conclusion: 1) GLUT4 is the major glucose transporter to be recruited to the surface of cardiomyocytes in response to insulin; 2) these cells express a high level of GLUT1; and 3) intracellular GLUT4-containing vesicles consist of at least two populations, which is compatible with recently proposed models of GLUT4 trafficking in adipocytes.
publishDate 1997
dc.date.none.fl_str_mv 1997
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/177124
url https://hdl.handle.net/2445/177124
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1074/jbc.272.11.7085
Journal of Biological Chemistry, 1997, vol. 272, num. 11, p. 7085-7092
https://doi.org/10.1074/jbc.272.11.7085
dc.rights.none.fl_str_mv (c) American Society for Biochemistry and Molecular Biology, 1997
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) American Society for Biochemistry and Molecular Biology, 1997
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 8 p.
application/pdf
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv Articles publicats en revistes (Ciències Fisiològiques)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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