Recovery and determination of cholesterol-lowering compounds from Olea europaea seeds employing pressurized liquid extraction and gas chromatography-mass spectrometry
Our previous work demonstrated the presence of compounds with hypocholesterolemic capacity in olive seeds. These compounds were extracted using CO2-expanded ethyl acetate and identified as tyrosol, hydroxytyrosol, and beta-sitosterol using GC&#-MS. This work describes the extraction of these com...
| Authors: | , , , |
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| Format: | article |
| Publication Date: | 2020 |
| Country: | España |
| Institution: | Universidad de Alcalá (UAH) |
| Repository: | e_Buah Biblioteca Digital Universidad de Alcalá |
| Language: | English |
| OAI Identifier: | oai:ebuah.uah.es:10017/44501 |
| Online Access: | http://hdl.handle.net/10017/44501 https://dx.doi.org/10.1016/j.microc.2020.104812 |
| Access Level: | Open access |
| Keyword: | Cholesterol-lowering capacity Hansen solubility parameters Olive seed Phenolic compounds Phytosterols Pressurized liquid extraction Química Chemistry |
| Summary: | Our previous work demonstrated the presence of compounds with hypocholesterolemic capacity in olive seeds. These compounds were extracted using CO2-expanded ethyl acetate and identified as tyrosol, hydroxytyrosol, and beta-sitosterol using GC&#-MS. This work describes the extraction of these compounds from different olive seeds using pressurized ethyl acetate. Their solubility in ethyl acetate at temperatures ranging from 40&-200 °C was theoretically predicted by Hansen solubility parameters. The content of these compounds was estimated by GC&#-MS, as well as, the reduction of the micellar cholesterol solubility (RMCS) capacity of extracts enabling to establish the optimum extraction temperature at 100 °C. A GC&-MS method was developed and validated in terms of its analytical characteristics for a sensitive determination and quantification of tyrosol, hydroxytyrosol, and &;946#sitosterol in different olive seeds. Within varieties, Manzanilla seeds presented the highest concentration of tyrosol, hydroxytyrosol, and the lowest concentration of beta-sitosterol, as well as the highest RMCS capacity |
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