Identification of secreted Candida proteins using mass spectrometry
Analysis of fungal secretomes using mass spectrometry is a useful technique in cell biology. Knowledge of the secretome of a human fungal pathogen may yield important information of hostpathogen interactions and way be useful for identifying vaccines candidates or diagnostic markers for antifungal s...
| Autores: | , , , |
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| Tipo de recurso: | capítulo de libro |
| Fecha de publicación: | 2016 |
| País: | España |
| Institución: | Universidad de Castilla-La Mancha |
| Repositorio: | RUIdeRA. Repositorio Institucional de la UCLM |
| OAI Identifier: | oai:ruidera.uclm.es:10578/43657 |
| Acceso en línea: | https://pubmed.ncbi.nlm.nih.gov/26519067/ https://hdl.handle.net/10578/43657 |
| Access Level: | acceso abierto |
| Palabra clave: | Candida LC/MS/MS Mass spectrometry Secretome Signal peptide Trypsin |
| Sumario: | Analysis of fungal secretomes using mass spectrometry is a useful technique in cell biology. Knowledge of the secretome of a human fungal pathogen may yield important information of hostpathogen interactions and way be useful for identifying vaccines candidates or diagnostic markers for antifungal strategies. In this chapter, with a main focus on sample preparation aspects, we describe the methodology that we apply for gel-independent batch identification and quantification of proteins that are secreted during growth in liquid cultures. |
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