A diet enriched with cocoa prevents IgE synthesis in a rat allergy model

Previous studies in young rats reported the impact of cocoa intake on healthy immune status and allow suggesting it may have a role in the prevention of some immune-mediated diseases. The aim of this study was to ascertain the effect of a cocoa diet in a model of allergy in young rats. Three-week-ol...

Descripción completa

Detalles Bibliográficos
Autores: Abril Gil, Maria del Mar, Massot Cladera, Malen, Pérez-Cano, Francisco J., Castellote i Bargalló, M. Cristina, Franch i Masferrer, Àngels, Castell, Margarida
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2012
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/33013
Acceso en línea:https://hdl.handle.net/2445/33013
Access Level:acceso abierto
Palabra clave:Cacau
Flavonoides
Al·lèrgia
Rates (Animals de laboratori)
Resposta immunitària
Immunoglobulina E
Cocoa
Flavonoids
Allergy
Rats as laboratory animals
Immune response
Immunoglobulin E
id ES_43e7a0ceced53dfdd28c2efeac39d269
oai_identifier_str oai:diposit.ub.edu:2445/33013
network_acronym_str ES
network_name_str España
repository_id_str
spelling A diet enriched with cocoa prevents IgE synthesis in a rat allergy modelAbril Gil, Maria del MarMassot Cladera, MalenPérez-Cano, Francisco J.Castellote i Bargalló, M. CristinaFranch i Masferrer, ÀngelsCastell, MargaridaCacauFlavonoidesAl·lèrgiaRates (Animals de laboratori)Resposta immunitàriaImmunoglobulina ECocoaFlavonoidsAllergyRats as laboratory animalsImmune responseImmunoglobulin EPrevious studies in young rats reported the impact of cocoa intake on healthy immune status and allow suggesting it may have a role in the prevention of some immune-mediated diseases. The aim of this study was to ascertain the effect of a cocoa diet in a model of allergy in young rats. Three-week-old Brown Norway rats were immunized by i.p. injection of ovalbumin (OVA) with alum as adjuvant and Bordetella pertussis toxin. During the next 4 weeks rats received either a cocoa diet (containing 0.2% polyphenols, w/w) or a standard diet. Animals fed a standard diet showed high concentrations of anti-OVA IgG1, IgG2a, IgG2b and high anti-OVA IgE titres, which is the antibody involved in allergic response. In contrast, animals fed a cocoa diet showed significantly lower concentrations of anti-OVA IgG1 and IgG2a antibodies. Interestingly, the cocoa diet prevented anti-OVA IgE synthesis and decreased total serum IgE concentration. Analysis of cytokine production in lymph node cells at the end of the study revealed that, in this compartment, the cocoa diet decreased the tumor necrosis factor (TNF) - alpha and the interleukin (IL) -10 secretion but not IL-4 production. In conclusion, a cocoa-enriched diet in young rats produces an immunomodulatory effect that prevents anti-allergen IgE synthesis, suggesting a potential role for cocoa flavonoids in the prevention or treatment of allergic diseases.Elsevier B.V.2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/33013Articles publicats en revistes (Bioquímica i Fisiologia)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: http://dx.doi.org/10.1016/j.phrs.2012.02.001Pharmacological Research, 2012, vol. 65, num. 6, p. 603-608http://dx.doi.org/10.1016/j.phrs.2012.02.001(c) Elsevier B.V., 2012info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/330132026-05-27T06:46:51Z
dc.title.none.fl_str_mv A diet enriched with cocoa prevents IgE synthesis in a rat allergy model
title A diet enriched with cocoa prevents IgE synthesis in a rat allergy model
spellingShingle A diet enriched with cocoa prevents IgE synthesis in a rat allergy model
Abril Gil, Maria del Mar
Cacau
Flavonoides
Al·lèrgia
Rates (Animals de laboratori)
Resposta immunitària
Immunoglobulina E
Cocoa
Flavonoids
Allergy
Rats as laboratory animals
Immune response
Immunoglobulin E
title_short A diet enriched with cocoa prevents IgE synthesis in a rat allergy model
title_full A diet enriched with cocoa prevents IgE synthesis in a rat allergy model
title_fullStr A diet enriched with cocoa prevents IgE synthesis in a rat allergy model
title_full_unstemmed A diet enriched with cocoa prevents IgE synthesis in a rat allergy model
title_sort A diet enriched with cocoa prevents IgE synthesis in a rat allergy model
dc.creator.none.fl_str_mv Abril Gil, Maria del Mar
Massot Cladera, Malen
Pérez-Cano, Francisco J.
Castellote i Bargalló, M. Cristina
Franch i Masferrer, Àngels
Castell, Margarida
author Abril Gil, Maria del Mar
author_facet Abril Gil, Maria del Mar
Massot Cladera, Malen
Pérez-Cano, Francisco J.
Castellote i Bargalló, M. Cristina
Franch i Masferrer, Àngels
Castell, Margarida
author_role author
author2 Massot Cladera, Malen
Pérez-Cano, Francisco J.
Castellote i Bargalló, M. Cristina
Franch i Masferrer, Àngels
Castell, Margarida
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Cacau
Flavonoides
Al·lèrgia
Rates (Animals de laboratori)
Resposta immunitària
Immunoglobulina E
Cocoa
Flavonoids
Allergy
Rats as laboratory animals
Immune response
Immunoglobulin E
topic Cacau
Flavonoides
Al·lèrgia
Rates (Animals de laboratori)
Resposta immunitària
Immunoglobulina E
Cocoa
Flavonoids
Allergy
Rats as laboratory animals
Immune response
Immunoglobulin E
description Previous studies in young rats reported the impact of cocoa intake on healthy immune status and allow suggesting it may have a role in the prevention of some immune-mediated diseases. The aim of this study was to ascertain the effect of a cocoa diet in a model of allergy in young rats. Three-week-old Brown Norway rats were immunized by i.p. injection of ovalbumin (OVA) with alum as adjuvant and Bordetella pertussis toxin. During the next 4 weeks rats received either a cocoa diet (containing 0.2% polyphenols, w/w) or a standard diet. Animals fed a standard diet showed high concentrations of anti-OVA IgG1, IgG2a, IgG2b and high anti-OVA IgE titres, which is the antibody involved in allergic response. In contrast, animals fed a cocoa diet showed significantly lower concentrations of anti-OVA IgG1 and IgG2a antibodies. Interestingly, the cocoa diet prevented anti-OVA IgE synthesis and decreased total serum IgE concentration. Analysis of cytokine production in lymph node cells at the end of the study revealed that, in this compartment, the cocoa diet decreased the tumor necrosis factor (TNF) - alpha and the interleukin (IL) -10 secretion but not IL-4 production. In conclusion, a cocoa-enriched diet in young rats produces an immunomodulatory effect that prevents anti-allergen IgE synthesis, suggesting a potential role for cocoa flavonoids in the prevention or treatment of allergic diseases.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/33013
url https://hdl.handle.net/2445/33013
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: http://dx.doi.org/10.1016/j.phrs.2012.02.001
Pharmacological Research, 2012, vol. 65, num. 6, p. 603-608
http://dx.doi.org/10.1016/j.phrs.2012.02.001
dc.rights.none.fl_str_mv (c) Elsevier B.V., 2012
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Elsevier B.V., 2012
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Fisiologia)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869407056327344128
score 15,301603

Similares en LA Referencia