Investigating intracellular persistence of Staphylococcus aureus within a murine alveolar macrophage cell line

Objective: Staphylococcus aureus is a particularly difficult pathogen to eradicate from the respiratory tract. Previous studies have highlighted the intracellular capacity of S. aureus in several phagocytic and non-phagocytic cells. The aim of this study was to define S. aureus interaction within a...

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Autores: Lacoma, A, Cano, V, Moranta, D, Regueiro, V, Dominguez-Villanueva, D, Laabei, M, González-Nicolau, María Del Mar, Ausina, V, Prat, C, Bengoechea, Jose Antonio
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/20483
Acceso en línea:http://hdl.handle.net/20.500.12105/20483
Access Level:acceso abierto
Palabra clave:Invasion
Macrophage
Persistence
Phagocytosis
Staphylococcus aureus
Viabilidad Microbiana
Animales
Macrófagos Alveolares
Infecciones Estafilocócicas
Fagocitosis
Ratones
Línea Celular
Animals
Macrophages, Alveolar
Microbial Viability
Cell Line
Staphylococcal Infections
Mice
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spelling Investigating intracellular persistence of Staphylococcus aureus within a murine alveolar macrophage cell lineLacoma, ACano, VMoranta, DRegueiro, VDominguez-Villanueva, DLaabei, MGonzález-Nicolau, María Del MarAusina, VPrat, CBengoechea, Jose AntonioInvasionMacrophagePersistencePhagocytosisStaphylococcus aureusViabilidad MicrobianaAnimalesMacrófagos AlveolaresStaphylococcus aureusInfecciones EstafilocócicasFagocitosisRatonesLínea CelularPhagocytosisAnimalsMacrophages, AlveolarMicrobial ViabilityCell LineStaphylococcal InfectionsStaphylococcus aureusMiceObjective: Staphylococcus aureus is a particularly difficult pathogen to eradicate from the respiratory tract. Previous studies have highlighted the intracellular capacity of S. aureus in several phagocytic and non-phagocytic cells. The aim of this study was to define S. aureus interaction within a murine alveolar macrophage cell line. Methods: Cell line MH-S was infected with Newman strain. Molecular mechanisms involved in phagocytosis were explored. To assess whether S. aureus survives intracellularly quantitative (gentamicin protection assays and bacterial plating) and qualitative analysis (immunofluorescence microscopy) were performed. Bacterial colocalization with different markers of the endocytic pathway was examined to characterize its intracellular trafficking. Results: We found that S. aureus uptake requires host actin polymerization, microtubule assembly and activation of phosphatidylinositol 3-kinase signaling. Time course experiments showed that Newman strain was able to persist within macrophages at least until 28.5 h post infection. We observed that intracellular bacteria are located inside an acidic subcellular compartment, which co-localizes with the late endosome/lysosome markers Lamp-1, Rab7 and RILP. Colocalization counts with TMR-dextran might reflect a balance between bacterial killing and intracellular survival. Conclusions: This study indicates that S. aureus persists and replicates inside murine alveolar macrophages, representing a privileged niche that can potentially offer protection from antimicrobial activity and immunological host defense mechanisms.Taylor & Francis20242024-07-1120172017-01-0120172017-01-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articlehttp://hdl.handle.net/20.500.12105/20483reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/204832026-06-12T12:43:37Z
dc.title.none.fl_str_mv Investigating intracellular persistence of Staphylococcus aureus within a murine alveolar macrophage cell line
title Investigating intracellular persistence of Staphylococcus aureus within a murine alveolar macrophage cell line
spellingShingle Investigating intracellular persistence of Staphylococcus aureus within a murine alveolar macrophage cell line
Lacoma, A
Invasion
Macrophage
Persistence
Phagocytosis
Staphylococcus aureus
Viabilidad Microbiana
Animales
Macrófagos Alveolares
Staphylococcus aureus
Infecciones Estafilocócicas
Fagocitosis
Ratones
Línea Celular
Phagocytosis
Animals
Macrophages, Alveolar
Microbial Viability
Cell Line
Staphylococcal Infections
Staphylococcus aureus
Mice
title_short Investigating intracellular persistence of Staphylococcus aureus within a murine alveolar macrophage cell line
title_full Investigating intracellular persistence of Staphylococcus aureus within a murine alveolar macrophage cell line
title_fullStr Investigating intracellular persistence of Staphylococcus aureus within a murine alveolar macrophage cell line
title_full_unstemmed Investigating intracellular persistence of Staphylococcus aureus within a murine alveolar macrophage cell line
title_sort Investigating intracellular persistence of Staphylococcus aureus within a murine alveolar macrophage cell line
dc.creator.none.fl_str_mv Lacoma, A
Cano, V
Moranta, D
Regueiro, V
Dominguez-Villanueva, D
Laabei, M
González-Nicolau, María Del Mar
Ausina, V
Prat, C
Bengoechea, Jose Antonio
author Lacoma, A
author_facet Lacoma, A
Cano, V
Moranta, D
Regueiro, V
Dominguez-Villanueva, D
Laabei, M
González-Nicolau, María Del Mar
Ausina, V
Prat, C
Bengoechea, Jose Antonio
author_role author
author2 Cano, V
Moranta, D
Regueiro, V
Dominguez-Villanueva, D
Laabei, M
González-Nicolau, María Del Mar
Ausina, V
Prat, C
Bengoechea, Jose Antonio
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Invasion
Macrophage
Persistence
Phagocytosis
Staphylococcus aureus
Viabilidad Microbiana
Animales
Macrófagos Alveolares
Staphylococcus aureus
Infecciones Estafilocócicas
Fagocitosis
Ratones
Línea Celular
Phagocytosis
Animals
Macrophages, Alveolar
Microbial Viability
Cell Line
Staphylococcal Infections
Staphylococcus aureus
Mice
topic Invasion
Macrophage
Persistence
Phagocytosis
Staphylococcus aureus
Viabilidad Microbiana
Animales
Macrófagos Alveolares
Staphylococcus aureus
Infecciones Estafilocócicas
Fagocitosis
Ratones
Línea Celular
Phagocytosis
Animals
Macrophages, Alveolar
Microbial Viability
Cell Line
Staphylococcal Infections
Staphylococcus aureus
Mice
description Objective: Staphylococcus aureus is a particularly difficult pathogen to eradicate from the respiratory tract. Previous studies have highlighted the intracellular capacity of S. aureus in several phagocytic and non-phagocytic cells. The aim of this study was to define S. aureus interaction within a murine alveolar macrophage cell line. Methods: Cell line MH-S was infected with Newman strain. Molecular mechanisms involved in phagocytosis were explored. To assess whether S. aureus survives intracellularly quantitative (gentamicin protection assays and bacterial plating) and qualitative analysis (immunofluorescence microscopy) were performed. Bacterial colocalization with different markers of the endocytic pathway was examined to characterize its intracellular trafficking. Results: We found that S. aureus uptake requires host actin polymerization, microtubule assembly and activation of phosphatidylinositol 3-kinase signaling. Time course experiments showed that Newman strain was able to persist within macrophages at least until 28.5 h post infection. We observed that intracellular bacteria are located inside an acidic subcellular compartment, which co-localizes with the late endosome/lysosome markers Lamp-1, Rab7 and RILP. Colocalization counts with TMR-dextran might reflect a balance between bacterial killing and intracellular survival. Conclusions: This study indicates that S. aureus persists and replicates inside murine alveolar macrophages, representing a privileged niche that can potentially offer protection from antimicrobial activity and immunological host defense mechanisms.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-01-01
2017
2017-01-01
2024
2024-07-11
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/20483
url http://hdl.handle.net/20.500.12105/20483
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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