Foodborne Nosocomial Outbreak of SHV1 and CTX-M-15–producing Klebsiella pneumoniae: Epidemiology and Control

Background. We describe a foodborne nosocomial outbreak due to extended-spectrum b-lactamase (ESBL)–producing Klebsiella pneumoniae. Methods. An outbreak of ESBL K. pneumoniae was detected in March 2008. Initial control measures included contact isolation and a protocol for routine detection and rei...

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Detalhes bibliográficos
Autores: Calbo Sebastian, Esther, Freixas, Núria, Xercavins, Mariona, Riera, Montserrat, Nicolás, Carme, Monistrol Ruano, Olga, Solé, Maria Del Mar, Sala i Farré, M Rosa, Vila, Jordi, Garau, Javier
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2011
País:España
Recursos:Universitat de Lleida (UdL)
Repositorio:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/467244
Acesso em linha:https://doi.org/10.1093/cid/ciq238
https://hdl.handle.net/10459.1/467244
Access Level:acceso abierto
Palavra-chave:Epidemiology
Disease outbreaks
Feces
Food
Genes
Inpatients
Klebsiella pneumoniae
Infections
Extended-spectrum beta lactamases
Microbial colonization
Descrição
Resumo:Background. We describe a foodborne nosocomial outbreak due to extended-spectrum b-lactamase (ESBL)–producing Klebsiella pneumoniae. Methods. An outbreak of ESBL K. pneumoniae was detected in March 2008. Initial control measures included contact isolation and a protocol for routine detection and reinforcement in hand hygiene practices. ESBL producers were screened for the blaTEM, blaSHV, and blaCTX-M genes. Pulsed-field gel electrophoresis analysis was performed using XbaI as a restriction endonuclease. Results. One hundred fifty-six colonized and/or infected patients were identified, 35 (22.4%) of whom had infection. The outbreak affected all hospital wards. Fecal carriage was up to 38% of patients in some wards. Of note, investigation revealed a very short delay between admission and colonization. None of the health care workers or environmental surfaces in the wards was found to be colonized. This prompted an epidemiological investigation of a possible foodborne transmission. We found that up to 35% of the hospital kitchen–screened surfaces or foodstuff were colonized and that 6 (14%) of 44 food handlers were found to be fecal carriers. Phenotypic and genotypic analysis of all clinical, environmental, and fecal carrier isolates showed the dissemination of a single strain of SHV-1 and CTX-M-15–producing K. pneumoniae. At that time, structural and functional reforms in the kitchen were performed. These were followed by a progressive reduction in colonization and infection rates among inpatients until complete control was obtained in December 2008. No restrictions in the use of antibiotics were needed. Conclusions. To our knowledge, this is the first reported hospital outbreak that provides evidence that food can be a transmission vector for ESBL K. pneumoniae.