Soft topographical patterns trigger a stiffness-dependent cellular response to contact guidance

Topographical patterns are a powerful tool to study directional migration. Grooved substrates have been extensively used as in vitro models of aligned extracellular matrix fibers because they induce cell elongation, alignment, and migration through a phenomenon known as contact guidance. This proces...

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Detalhes bibliográficos
Autores: Comelles Pujadas, Jordi, Fernández Majada, Vanesa, Acevedo, Verónica, Rebollo Calderón, Beatriz, Martínez Fraiz, Elena
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2022
País:España
Recursos:Universidad de Barcelona
Repositório:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/198767
Acesso em linha:https://hdl.handle.net/2445/198767
Access Level:Acceso aberto
Palavra-chave:Migració cel·lular
Topografia
Citosquelet
Fibroblasts
Cell migration
Topography
Cytoskeleton
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spelling Soft topographical patterns trigger a stiffness-dependent cellular response to contact guidanceComelles Pujadas, JordiFernández Majada, VanesaAcevedo, VerónicaRebollo Calderón, BeatrizMartínez Fraiz, ElenaMigració cel·lularTopografiaCitosqueletFibroblastsCell migrationTopographyCytoskeletonFibroblastsTopographical patterns are a powerful tool to study directional migration. Grooved substrates have been extensively used as in vitro models of aligned extracellular matrix fibers because they induce cell elongation, alignment, and migration through a phenomenon known as contact guidance. This process, which involves the orientation of focal adhesions, F-actin, and microtubule cytoskeleton along the direction of the grooves, has been primarily studied on hard materials of non-physiological stiffness. But how it unfolds when the stiffness of the grooves varies within the physiological range is less known. Here we show that substrate stiffness modulates the cellular response to topographical contact guidance. We find that for fibroblasts, while focal adhesions and actin respond to topography independently of the stiffness, microtubules show a stiffness-dependent response that regulates contact guidance. On the other hand, both clusters and single breast carcinoma epithelial cells display stiffnessdependent contact guidance, leading to more directional and efficient migration when increasing substrate stiffness. These results suggest that both matrix stiffening and alignment of extracellular matrix fibers cooperate during directional cell migration, and that the outcome differs between cell types depending on how they organize their cytoskeletons.Elsevier2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/198767Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1016/j.mtbio.2023.100593Materials Today Bio, 2022, vol. 19, num. 100593https://doi.org/10.1016/j.mtbio.2023.100593cc-by (c) Comelles, Jordi et al., 2022https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1987672026-05-27T06:46:51Z
dc.title.none.fl_str_mv Soft topographical patterns trigger a stiffness-dependent cellular response to contact guidance
title Soft topographical patterns trigger a stiffness-dependent cellular response to contact guidance
spellingShingle Soft topographical patterns trigger a stiffness-dependent cellular response to contact guidance
Comelles Pujadas, Jordi
Migració cel·lular
Topografia
Citosquelet
Fibroblasts
Cell migration
Topography
Cytoskeleton
Fibroblasts
title_short Soft topographical patterns trigger a stiffness-dependent cellular response to contact guidance
title_full Soft topographical patterns trigger a stiffness-dependent cellular response to contact guidance
title_fullStr Soft topographical patterns trigger a stiffness-dependent cellular response to contact guidance
title_full_unstemmed Soft topographical patterns trigger a stiffness-dependent cellular response to contact guidance
title_sort Soft topographical patterns trigger a stiffness-dependent cellular response to contact guidance
dc.creator.none.fl_str_mv Comelles Pujadas, Jordi
Fernández Majada, Vanesa
Acevedo, Verónica
Rebollo Calderón, Beatriz
Martínez Fraiz, Elena
author Comelles Pujadas, Jordi
author_facet Comelles Pujadas, Jordi
Fernández Majada, Vanesa
Acevedo, Verónica
Rebollo Calderón, Beatriz
Martínez Fraiz, Elena
author_role author
author2 Fernández Majada, Vanesa
Acevedo, Verónica
Rebollo Calderón, Beatriz
Martínez Fraiz, Elena
author2_role author
author
author
author
dc.subject.none.fl_str_mv Migració cel·lular
Topografia
Citosquelet
Fibroblasts
Cell migration
Topography
Cytoskeleton
Fibroblasts
topic Migració cel·lular
Topografia
Citosquelet
Fibroblasts
Cell migration
Topography
Cytoskeleton
Fibroblasts
description Topographical patterns are a powerful tool to study directional migration. Grooved substrates have been extensively used as in vitro models of aligned extracellular matrix fibers because they induce cell elongation, alignment, and migration through a phenomenon known as contact guidance. This process, which involves the orientation of focal adhesions, F-actin, and microtubule cytoskeleton along the direction of the grooves, has been primarily studied on hard materials of non-physiological stiffness. But how it unfolds when the stiffness of the grooves varies within the physiological range is less known. Here we show that substrate stiffness modulates the cellular response to topographical contact guidance. We find that for fibroblasts, while focal adhesions and actin respond to topography independently of the stiffness, microtubules show a stiffness-dependent response that regulates contact guidance. On the other hand, both clusters and single breast carcinoma epithelial cells display stiffnessdependent contact guidance, leading to more directional and efficient migration when increasing substrate stiffness. These results suggest that both matrix stiffening and alignment of extracellular matrix fibers cooperate during directional cell migration, and that the outcome differs between cell types depending on how they organize their cytoskeletons.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/198767
url https://hdl.handle.net/2445/198767
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1016/j.mtbio.2023.100593
Materials Today Bio, 2022, vol. 19, num. 100593
https://doi.org/10.1016/j.mtbio.2023.100593
dc.rights.none.fl_str_mv cc-by (c) Comelles, Jordi et al., 2022
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Comelles, Jordi et al., 2022
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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