Regulation of alternative splicing by the p38 SAPK in response to stress

Cells have the ability to respond and adapt to environmental changes through activation of stress-activated protein kinases (SAPKs). Activation of the p38 SAPK is critical to elicit the early gene expression program required for cell adaptation to stress. Alternative splicing (AS) is a crucial mecha...

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Detalles Bibliográficos
Autor: Carbonell Núñez, Caterina
Tipo de recurso: tesis doctoral
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:CBUC, CESCA
Repositorio:TDR. Tesis Doctorales en Red
OAI Identifier:oai:www.tdx.cat:10803/664502
Acceso en línea:http://hdl.handle.net/10803/664502
Access Level:acceso abierto
Palabra clave:P38 SAPK
Alternative pre-mRNA splicing
Cell stress
Spliceosome
Processament alternatiu del pre-ARNm
Estrés cel.lular
Espliceosoma
576
Descripción
Sumario:Cells have the ability to respond and adapt to environmental changes through activation of stress-activated protein kinases (SAPKs). Activation of the p38 SAPK is critical to elicit the early gene expression program required for cell adaptation to stress. Alternative splicing (AS) is a crucial mechanism for gene regulation that has been shown to be modulated in response to a wide range of extracellular stimuli. However, the mechanisms behind AS regulation and the functional consequences of differential isoform expression in stress conditions remain largely unknown. In this study, we identified novel associations between p38 and the splicing machinery, providing mechanisms by which p38 regulates AS. Furthermore, characterization of protein isoforms produced by p38-dependent AS give an insight into the biological relevance of this regulation in adaptation to environmental stresses. Altogether, the results of this thesis highlight a role for SAPK signaling pathways in adaptation to environmental changes through AS modulation.