A New Biostimulant Derived from Soybean by-products Enhances Plant Tolerance to Abiotic Stress Triggered by Ozone

Background: Tropospheric ozone is an air pollutant that causes negative effects on vegetation, leading to significant losses in crop productivity. It is generated by chemical reactions in the presence of sunlight between primary pollutants resulting from human activity, such as nitrogen oxides and v...

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Autores: Orts, A., Navarro Torre, Salvadora, Macías Benitez, S., Orts Gómez, José María, Naranjo Fernández, Emilia, Castaño Navarro, Angélica, Parrado Rubio, Juan
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/165053
Acceso en línea:https://hdl.handle.net/11441/165053
https://doi.org/10.1186/s12870-024-05290-3
Access Level:acceso abierto
Palabra clave:Biostimulant
Capsicum
Enzymatic extract
Okara
Ozone
ROS
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spelling A New Biostimulant Derived from Soybean by-products Enhances Plant Tolerance to Abiotic Stress Triggered by OzoneOrts, A.Navarro Torre, SalvadoraMacías Benitez, S.Orts Gómez, José MaríaNaranjo Fernández, EmiliaCastaño Navarro, AngélicaParrado Rubio, JuanBiostimulantCapsicumEnzymatic extractOkaraOzoneROSBackground: Tropospheric ozone is an air pollutant that causes negative effects on vegetation, leading to significant losses in crop productivity. It is generated by chemical reactions in the presence of sunlight between primary pollutants resulting from human activity, such as nitrogen oxides and volatile organic compounds. Due to the constantly increasing emission of ozone precursors, together with the influence of a warming climate on ozone levels, crop losses may be aggravated in the future. Therefore, the search for solutions to mitigate these losses becomes a priority. Ozone-induced abiotic stress is mainly due to reactive oxygen species generated by the spontaneous decomposition of ozone once it reaches the apoplast. In this regard, compounds with antioxidant activity offer a viable option to alleviate ozone-induced damage. Using enzymatic technology, we have developed a process that enables the production of an extract with biostimulant properties from okara, an industrial soybean byproduct. The biostimulant, named as OEE (Okara Enzymatic Extract), is water-soluble and is enriched in bioactive compounds present in okara, such as isoflavones. Additionally, it contains a significant fraction of protein hydrolysates contributing to its functional effect. Given its antioxidant capacity, we aimed to investigate whether OEE could alleviate ozone-induced damage in plants. For that, pepper plants (Capsicum annuum) exposed to ozone were treated with a foliar application of OEE. Results: OEE mitigated ozone-induced damage, as evidenced by the net photosynthetic rate, electron transport rate, effective quantum yield of PSII, and delayed fluorescence. This protection was confirmed by the level of expression of genes associated with photosystem II. The beneficial effect was primarily due to its antioxidant activity, as evidenced by the lipid peroxidation rate measured through malondialdehyde content. Additionally, OEE triggered a mild oxidative response, indicated by increased activities of antioxidant enzymes in leaves (catalase, superoxide dismutase, and guaiacol peroxidase) and the oxidative stress index, providing further protection against ozone-induced stress. Conclusions: The present results support that OEE protects plants from ozone exposure. Taking into consideration that the promotion of plant resistance against abiotic damage is an important goal of biostimulants, we assume that its use as a new biostimulant could be considered.Ministerio de Ciencia e Innovación PID2021‑124964OB‑C21, TED2021‑129822B‑I00Springer NatureBioquímica y Biología MolecularMicrobiología y ParasitologíaMinisterio de Ciencia e Innovación (MICIN). España2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/165053https://doi.org/10.1186/s12870-024-05290-3reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésBMC Plant Biology, 24 (1), 580.PID2021‑124964OB‑C21TED2021‑129822B‑I00https://doi.org/10.1186/s12870-024-05290-3info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1650532026-06-17T12:51:07Z
dc.title.none.fl_str_mv A New Biostimulant Derived from Soybean by-products Enhances Plant Tolerance to Abiotic Stress Triggered by Ozone
title A New Biostimulant Derived from Soybean by-products Enhances Plant Tolerance to Abiotic Stress Triggered by Ozone
spellingShingle A New Biostimulant Derived from Soybean by-products Enhances Plant Tolerance to Abiotic Stress Triggered by Ozone
Orts, A.
Biostimulant
Capsicum
Enzymatic extract
Okara
Ozone
ROS
title_short A New Biostimulant Derived from Soybean by-products Enhances Plant Tolerance to Abiotic Stress Triggered by Ozone
title_full A New Biostimulant Derived from Soybean by-products Enhances Plant Tolerance to Abiotic Stress Triggered by Ozone
title_fullStr A New Biostimulant Derived from Soybean by-products Enhances Plant Tolerance to Abiotic Stress Triggered by Ozone
title_full_unstemmed A New Biostimulant Derived from Soybean by-products Enhances Plant Tolerance to Abiotic Stress Triggered by Ozone
title_sort A New Biostimulant Derived from Soybean by-products Enhances Plant Tolerance to Abiotic Stress Triggered by Ozone
dc.creator.none.fl_str_mv Orts, A.
Navarro Torre, Salvadora
Macías Benitez, S.
Orts Gómez, José María
Naranjo Fernández, Emilia
Castaño Navarro, Angélica
Parrado Rubio, Juan
author Orts, A.
author_facet Orts, A.
Navarro Torre, Salvadora
Macías Benitez, S.
Orts Gómez, José María
Naranjo Fernández, Emilia
Castaño Navarro, Angélica
Parrado Rubio, Juan
author_role author
author2 Navarro Torre, Salvadora
Macías Benitez, S.
Orts Gómez, José María
Naranjo Fernández, Emilia
Castaño Navarro, Angélica
Parrado Rubio, Juan
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Bioquímica y Biología Molecular
Microbiología y Parasitología
Ministerio de Ciencia e Innovación (MICIN). España
dc.subject.none.fl_str_mv Biostimulant
Capsicum
Enzymatic extract
Okara
Ozone
ROS
topic Biostimulant
Capsicum
Enzymatic extract
Okara
Ozone
ROS
description Background: Tropospheric ozone is an air pollutant that causes negative effects on vegetation, leading to significant losses in crop productivity. It is generated by chemical reactions in the presence of sunlight between primary pollutants resulting from human activity, such as nitrogen oxides and volatile organic compounds. Due to the constantly increasing emission of ozone precursors, together with the influence of a warming climate on ozone levels, crop losses may be aggravated in the future. Therefore, the search for solutions to mitigate these losses becomes a priority. Ozone-induced abiotic stress is mainly due to reactive oxygen species generated by the spontaneous decomposition of ozone once it reaches the apoplast. In this regard, compounds with antioxidant activity offer a viable option to alleviate ozone-induced damage. Using enzymatic technology, we have developed a process that enables the production of an extract with biostimulant properties from okara, an industrial soybean byproduct. The biostimulant, named as OEE (Okara Enzymatic Extract), is water-soluble and is enriched in bioactive compounds present in okara, such as isoflavones. Additionally, it contains a significant fraction of protein hydrolysates contributing to its functional effect. Given its antioxidant capacity, we aimed to investigate whether OEE could alleviate ozone-induced damage in plants. For that, pepper plants (Capsicum annuum) exposed to ozone were treated with a foliar application of OEE. Results: OEE mitigated ozone-induced damage, as evidenced by the net photosynthetic rate, electron transport rate, effective quantum yield of PSII, and delayed fluorescence. This protection was confirmed by the level of expression of genes associated with photosystem II. The beneficial effect was primarily due to its antioxidant activity, as evidenced by the lipid peroxidation rate measured through malondialdehyde content. Additionally, OEE triggered a mild oxidative response, indicated by increased activities of antioxidant enzymes in leaves (catalase, superoxide dismutase, and guaiacol peroxidase) and the oxidative stress index, providing further protection against ozone-induced stress. Conclusions: The present results support that OEE protects plants from ozone exposure. Taking into consideration that the promotion of plant resistance against abiotic damage is an important goal of biostimulants, we assume that its use as a new biostimulant could be considered.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/165053
https://doi.org/10.1186/s12870-024-05290-3
url https://hdl.handle.net/11441/165053
https://doi.org/10.1186/s12870-024-05290-3
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv BMC Plant Biology, 24 (1), 580.
PID2021‑124964OB‑C21
TED2021‑129822B‑I00
https://doi.org/10.1186/s12870-024-05290-3
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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