Chitosan-Mediated Expression of <em>Caenorhabditis elegans fat-1 </em>and <em>fat-2 </em>in <em>Sparus aurata</em>: Short-Term Effects on the Hepatic Fatty Acid Profile, Intermediary Metabolism, and Proinflammatory Factors

A single dose of chitosan-tripolyphosphate (TPP) nanoparticles carrying expression plasmids for fish codon-optimized <em>Caenorhabditis elegans fat-1</em> and <em>fat-2</em> was intraperitoneally administered to gilthead seabream (<em>Sparus aurata</em>) to stimul...

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Autores: Wu, Yuanbing, Rashidpour, Ania, Duan, Wenwen, Fábregas, A. (Anna), Almajano Pablos, Ma. Pilar (María Pilar), Metón Teijeiro, Isidoro
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/224579
Acceso en línea:https://hdl.handle.net/2445/224579
Access Level:acceso abierto
Palabra clave:Inflamació
Àcids grassos omega-3
Quitosan
Àcids grassos
Inflammation
Omega-3 fatty acids
Chitosan
Fatty acids
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spelling Chitosan-Mediated Expression of <em>Caenorhabditis elegans fat-1 </em>and <em>fat-2 </em>in <em>Sparus aurata</em>: Short-Term Effects on the Hepatic Fatty Acid Profile, Intermediary Metabolism, and Proinflammatory FactorsWu, YuanbingRashidpour, AniaDuan, WenwenFábregas, A. (Anna)Almajano Pablos, Ma. Pilar (María Pilar)Metón Teijeiro, IsidoroInflamacióÀcids grassos omega-3QuitosanÀcids grassosInflammationOmega-3 fatty acidsChitosanFatty acidsA single dose of chitosan-tripolyphosphate (TPP) nanoparticles carrying expression plasmids for fish codon-optimized <em>Caenorhabditis elegans fat-1</em> and <em>fat-2</em> was intraperitoneally administered to gilthead seabream (<em>Sparus aurata</em>) to stimulate the biosynthesis of omega-3 long-chain polyunsaturated fatty acids (<em>n</em>-3 LC-PUFA) and evaluate subsequent short-term effects on liver intermediary metabolism and immunity. Seventy-two hours post-injection, the upregulation of <em>fat-1</em> elevated eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and total <em>n</em>-3 fatty acids in the liver, while <em>fat-2</em> enhanced DHA and <em>n</em>-3 fatty acids. Co-expression of <em>fat-1</em> and <em>fat-2</em> increased EPA, DHA, PUFA, and the total <em>n</em>-6 and <em>n</em>-3 LC-PUFA, while reducing plasma triglycerides. The expression of <em>fat-1</em> and <em>fat-2</em> suppressed hepatic lipogenesis by downregulating <em>srebf1 </em>and <em>pparg</em>, and consequently key genes in fatty acid synthesis (<em>acaca</em>, <em>acacb</em>, <em>fasn</em>, <em>scd1</em>, and <em>fads2</em>). In contrast, the co-expression of <em>fat-1</em> and <em>fat-2</em> upregulated <em>hnf4a</em>, <em>chrebp</em>, and <em>pfkl</em>, a rate-limiting enzyme in glycolysis. Furthermore, <em>fat-1</em> and <em>fat-2</em> reduced hepatic proinflammatory markers such as <em>tnfa</em> and <em>nfkb1</em>. In addition to enhancing EPA and DHA biosynthesis, promoting glycolysis, and suppressing lipogenesis, our findings suggest that the short-term expression of <em>C. elegans fat-1</em> and <em>fat-2</em> in the liver may also reduce inflammation and, therefore, could impact the health and growth performance of cultured fish.MDPI2025202520252025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion20 p.application/pdfhttps://hdl.handle.net/2445/224579Articles publicats en revistes (Bioquímica i Fisiologia)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3390/md23110434Marine Drugs, 2025, vol. 23https://doi.org/10.3390/md23110434cc-by (c) Wu, Y. et al., 2025http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2245792026-05-29T05:05:01Z
dc.title.none.fl_str_mv Chitosan-Mediated Expression of <em>Caenorhabditis elegans fat-1 </em>and <em>fat-2 </em>in <em>Sparus aurata</em>: Short-Term Effects on the Hepatic Fatty Acid Profile, Intermediary Metabolism, and Proinflammatory Factors
title Chitosan-Mediated Expression of <em>Caenorhabditis elegans fat-1 </em>and <em>fat-2 </em>in <em>Sparus aurata</em>: Short-Term Effects on the Hepatic Fatty Acid Profile, Intermediary Metabolism, and Proinflammatory Factors
spellingShingle Chitosan-Mediated Expression of <em>Caenorhabditis elegans fat-1 </em>and <em>fat-2 </em>in <em>Sparus aurata</em>: Short-Term Effects on the Hepatic Fatty Acid Profile, Intermediary Metabolism, and Proinflammatory Factors
Wu, Yuanbing
Inflamació
Àcids grassos omega-3
Quitosan
Àcids grassos
Inflammation
Omega-3 fatty acids
Chitosan
Fatty acids
title_short Chitosan-Mediated Expression of <em>Caenorhabditis elegans fat-1 </em>and <em>fat-2 </em>in <em>Sparus aurata</em>: Short-Term Effects on the Hepatic Fatty Acid Profile, Intermediary Metabolism, and Proinflammatory Factors
title_full Chitosan-Mediated Expression of <em>Caenorhabditis elegans fat-1 </em>and <em>fat-2 </em>in <em>Sparus aurata</em>: Short-Term Effects on the Hepatic Fatty Acid Profile, Intermediary Metabolism, and Proinflammatory Factors
title_fullStr Chitosan-Mediated Expression of <em>Caenorhabditis elegans fat-1 </em>and <em>fat-2 </em>in <em>Sparus aurata</em>: Short-Term Effects on the Hepatic Fatty Acid Profile, Intermediary Metabolism, and Proinflammatory Factors
title_full_unstemmed Chitosan-Mediated Expression of <em>Caenorhabditis elegans fat-1 </em>and <em>fat-2 </em>in <em>Sparus aurata</em>: Short-Term Effects on the Hepatic Fatty Acid Profile, Intermediary Metabolism, and Proinflammatory Factors
title_sort Chitosan-Mediated Expression of <em>Caenorhabditis elegans fat-1 </em>and <em>fat-2 </em>in <em>Sparus aurata</em>: Short-Term Effects on the Hepatic Fatty Acid Profile, Intermediary Metabolism, and Proinflammatory Factors
dc.creator.none.fl_str_mv Wu, Yuanbing
Rashidpour, Ania
Duan, Wenwen
Fábregas, A. (Anna)
Almajano Pablos, Ma. Pilar (María Pilar)
Metón Teijeiro, Isidoro
author Wu, Yuanbing
author_facet Wu, Yuanbing
Rashidpour, Ania
Duan, Wenwen
Fábregas, A. (Anna)
Almajano Pablos, Ma. Pilar (María Pilar)
Metón Teijeiro, Isidoro
author_role author
author2 Rashidpour, Ania
Duan, Wenwen
Fábregas, A. (Anna)
Almajano Pablos, Ma. Pilar (María Pilar)
Metón Teijeiro, Isidoro
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Inflamació
Àcids grassos omega-3
Quitosan
Àcids grassos
Inflammation
Omega-3 fatty acids
Chitosan
Fatty acids
topic Inflamació
Àcids grassos omega-3
Quitosan
Àcids grassos
Inflammation
Omega-3 fatty acids
Chitosan
Fatty acids
description A single dose of chitosan-tripolyphosphate (TPP) nanoparticles carrying expression plasmids for fish codon-optimized <em>Caenorhabditis elegans fat-1</em> and <em>fat-2</em> was intraperitoneally administered to gilthead seabream (<em>Sparus aurata</em>) to stimulate the biosynthesis of omega-3 long-chain polyunsaturated fatty acids (<em>n</em>-3 LC-PUFA) and evaluate subsequent short-term effects on liver intermediary metabolism and immunity. Seventy-two hours post-injection, the upregulation of <em>fat-1</em> elevated eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and total <em>n</em>-3 fatty acids in the liver, while <em>fat-2</em> enhanced DHA and <em>n</em>-3 fatty acids. Co-expression of <em>fat-1</em> and <em>fat-2</em> increased EPA, DHA, PUFA, and the total <em>n</em>-6 and <em>n</em>-3 LC-PUFA, while reducing plasma triglycerides. The expression of <em>fat-1</em> and <em>fat-2</em> suppressed hepatic lipogenesis by downregulating <em>srebf1 </em>and <em>pparg</em>, and consequently key genes in fatty acid synthesis (<em>acaca</em>, <em>acacb</em>, <em>fasn</em>, <em>scd1</em>, and <em>fads2</em>). In contrast, the co-expression of <em>fat-1</em> and <em>fat-2</em> upregulated <em>hnf4a</em>, <em>chrebp</em>, and <em>pfkl</em>, a rate-limiting enzyme in glycolysis. Furthermore, <em>fat-1</em> and <em>fat-2</em> reduced hepatic proinflammatory markers such as <em>tnfa</em> and <em>nfkb1</em>. In addition to enhancing EPA and DHA biosynthesis, promoting glycolysis, and suppressing lipogenesis, our findings suggest that the short-term expression of <em>C. elegans fat-1</em> and <em>fat-2</em> in the liver may also reduce inflammation and, therefore, could impact the health and growth performance of cultured fish.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/224579
url https://hdl.handle.net/2445/224579
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/md23110434
Marine Drugs, 2025, vol. 23
https://doi.org/10.3390/md23110434
dc.rights.none.fl_str_mv cc-by (c) Wu, Y. et al., 2025
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Wu, Y. et al., 2025
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 20 p.
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Fisiologia)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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